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ORIGINAL ARTICLE
Year : 2016  |  Volume : 13  |  Issue : 5  |  Page : 405-412

Effect of Cyclosporin A and Angiotensin II on cytosolic calcium levels in primary human gingival fibroblasts


1 Department of Periodontology and Implantology, Centre for Regenerative Medicine and Stem Cell Research, Sri Ramachandra University, Chennai, Tamil Nadu, India
2 Centres for Indian Systems of Medicine Quality Assurance and Standardization, Sri Ramachandra University, Chennai, Tamil Nadu, India
3 Centre for Regenerative Medicine and Stem Cell Research, Central Research Facility, Sri Ramachandra University, Chennai, Tamil Nadu, India

Correspondence Address:
Suresh Ranga Rao
Department of Periodontology and Implantology, Faculty of Dental Sciences, Centre for Regenerative Medicine and Stem Cells Research, Sri Ramachandra University, Porur, Chennai - 600 116, Tamil Nadu
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1735-3327.192276

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Background: To evaluate the effect of Cyclosporin A (CsA) and angiotensin II (Ang II) on cytosolic calcium levels in cultured human gingival fibroblasts (HGFs). Materials and Methods: Healthy gingival samples from six volunteers were obtained, and primary HGFs were cultured. Cell viability and proliferation assay were performed to identify the ideal concentrations of CsA and Ang II. Cytosolic calcium levels in cultured gingival fibroblasts treated with CsA and Ang II were studied using colorimetric assay, confocal and fluorescence imaging. Statistical analyses were done using SPSS software and GraphPad Prism. Results: Higher levels of cytosolic levels were evident in cells treated with CsA and Ang II when compared to control group and was statistically significant (P < 0.05) in both colorimetric assay and confocal imaging. Fluorescent images of the cultured HGFs revealed the same. Conclusion: Thus calcium being a key player in major cellular functions, plays a major role in the pathogenesis of drug-induced gingival overgrowth.


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