Dental Research Journal

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 16  |  Issue : 1  |  Page : 24--28

Assessment of antimicrobial activity of different concentrations of Tinospora cordifolia against Streptococcus mutans: An in vitro study


Sankalp Agarwal1, Priyadarshini H Ramamurthy2, Bennete Fernandes2, Avita Rath2, Preena Sidhu2 
1 Public Health Dentist, Madhur Smile Dental Clinic, Bareily, Uttar Pradesh, India
2 Faculty of Dentistry, SEGi University, Selangor 47810, Malaysia

Correspondence Address:
Dr. Priyadarshini H Ramamurthy
Faculty of Dentistry, SEGi University, No. 9, Jalan Teknologi, PJU5, Kota Damansara, Petaling Jaya, Selangor 47810
Malaysia

Background: The antimicrobial property of Tinospora cordifolia has been tested against a variety of microorganisms in the literature. The present study aimed to assess the antimicrobial activity of different concentrations of commercially available T. cordifolia powder against Streptococcus mutans. Materials and Methods: An in vitro study was undertaken in which extract of T. cordifolia was obtained using 100% ethanol by maceration. Seven different concentrations were prepared and tested against S. mutans in brain–heart infusion agar medium. Plates were incubated aerobically at 37°C for 48 h, and zone of inhibition was measured using Vernier caliper. 0.2% chlorhexidine and dimethylformamide were used as positive and negative controls respectively. The data were analysed by descriptive analytic tests. Results: The maximum antibacterial activity of T. cordifolia was observed with a volume of 40 μl at 2% concentration with a zone of inhibition of 19 mm. A 30 μl volume of 0.2% chlorhexidine showed a zone of inhibition of 28 mm, and no zone of inhibition was observed with dimethylformamide. Conclusion: Tinospora exhibited antimicrobial activity against S. mutans. However, it needs to be confirmed further with in vivo studies.


How to cite this article:
Agarwal S, Ramamurthy PH, Fernandes B, Rath A, Sidhu P. Assessment of antimicrobial activity of different concentrations of Tinospora cordifolia against Streptococcus mutans: An in vitro study.Dent Res J 2019;16:24-28


How to cite this URL:
Agarwal S, Ramamurthy PH, Fernandes B, Rath A, Sidhu P. Assessment of antimicrobial activity of different concentrations of Tinospora cordifolia against Streptococcus mutans: An in vitro study. Dent Res J [serial online] 2019 [cited 2019 Mar 19 ];16:24-28
Available from: http://www.drjjournal.net/article.asp?issn=1735-3327;year=2019;volume=16;issue=1;spage=24;epage=28;aulast=Agarwal;type=0