Dental Research Journal

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 16  |  Issue : 2  |  Page : 81--86

Correlation between human neutrophil peptide 1–3 secretion and azurophilic granule (CD63) expression in early childhood caries


Muhammad Luthfi1, Darmawan Setijanto2, Markus Budi Rahardjo1, Retno Indrawati1, Priyawan Rachmadi3, Mieke Sylvia Margaretha Amiatun Ruth4, Yoes Prijatna Dachlan5 
1 Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
2 Department of Public Health, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
3 Department of Dental Material, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
4 Department of Forensic Odontology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
5 Department of Parasitology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Correspondence Address:
Dr. Muhammad Luthfi
Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Mayjen Prof. Dr. Moestopo 47, Surabaya 60132
Indonesia

Background: In saliva, neutrophil constitutes the most prominent first-line defense of immune cells against pathogenic microbes. The importance of neutrophils to the host immune systems of neutropenic or patients disabled with regard to their neutrophil function results in a tendency toward serious infections, such as early childhood caries (ECC). The cytoplasmic granules present in neutrophils play a major role in neutrophil-mediated inflammation. Azurophilic granules contain antimicrobial proteins, such as defensin, a human antimicrobial peptide (HNP 1–3). The aim of this study is to analyze the correlation of HNP 1–3 secretion with CD63 expression on the surface of salivary neutrophils. Materials and Methods: This study constituted a cross-sectional, analytical observational study. Saliva taken from preschoolchildren between the ages of 4–6 years who had been divided into two groups, i.e., early childhood caries group with decayed, extracted, filled teeth (def-t) index >6 and caries free with def-t = 0, was subjected to a HNP 1–3 secretion test using ELISA assay and an expression test for CD63 by means of a flow cytometry test. The results obtained were analyzed using independent t-test and Pearson correlation (P < 0.05). Results: The secretion of HNP 1–3 in the saliva of ECC was higher (172.6 ± 41.64) compared to that of caries-free cases (140.39 ± 31.91), whereas the level of CD63 salivary expression in ECC was lower (2.32 ± 0.57) than in the presence of caries (2.67 ± 0.46). Conclusion: In ECC cases, saliva increases HNP 1–3 secretion but decreases CD63 expression on the surface of salivary neutrophils.


How to cite this article:
Luthfi M, Setijanto D, Rahardjo MB, Indrawati R, Rachmadi P, Ruth MS, Dachlan YP. Correlation between human neutrophil peptide 1–3 secretion and azurophilic granule (CD63) expression in early childhood caries.Dent Res J 2019;16:81-86


How to cite this URL:
Luthfi M, Setijanto D, Rahardjo MB, Indrawati R, Rachmadi P, Ruth MS, Dachlan YP. Correlation between human neutrophil peptide 1–3 secretion and azurophilic granule (CD63) expression in early childhood caries. Dent Res J [serial online] 2019 [cited 2019 Jul 21 ];16:81-86
Available from: http://www.drjjournal.net/article.asp?issn=1735-3327;year=2019;volume=16;issue=2;spage=81;epage=86;aulast=Luthfi;type=0