|Year : 2016 | Volume
| Issue : 1 | Page : 24-29
The effect of low-level helium-neon laser on oral wound healing
Farimah Sardari1, Farzaneh Ahrari2
1 Department of Oral Medicine, School of Dentistry, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
2 Dental Research Center, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran
|Date of Web Publication||22-Jan-2016|
Department of Oral Medicine, School of Dentistry, Rafsanjan University of Medical Sciences, Rafsanjan
Source of Support: None, Conflict of Interest: None
Background: The effectiveness of low power lasers on incisional wound healing, because of conflicting results of previous studies, is uncertain. Therefore, the aim of this study was to evaluate the effects of low-level helium-neon (He-Ne) laser irradiation on wound healing in rat's oral mucosa.
Materials and Methods: Sixty-four standardized incisions were carried out on the buccal mucosa of 32 male Wistar divided into four groups of eight animals each. Each rat received two incisions on the opposite sides of the buccal mucosa by a steel scalpel. On the right side (test side), a He-Ne laser (632 nm) was employed on the incision for 40 s. Laser radiation was used just in 1 st day, 1 st and 2 nd day, 1 st and 3 rd day, and continuous 3 days in groups of A, B, C, and D of rats, respectively. The left side (control side) did not receive any laser. Histological processing and hematoxylin and eosin staining were done on tissue samples after 5 days. Wilcoxon and Kruskal-Wallis tests were used for statistical analysis.
Results: Histological analysis showed that the tissue healing after continuous 3 days on the laser irradiated side was better than the control side, but there was no difference between the two sides in each groups (P > 0.05).
Conclusion: This study showed that He-Ne laser had no beneficial effects on incisional oral wound healing particularly in 5 days after laser therapy. Future research in the field of laser effects on oral wound healing in human is recommended.
Keywords: Lasers, Helium, Histological Technique, Low-level laser therapies, oral, wound healings
|How to cite this article:|
Sardari F, Ahrari F. The effect of low-level helium-neon laser on oral wound healing. Dent Res J 2016;13:24-9
| Introduction|| |
The process of tissue healing is very complex and involves vascular and cellular changes, epithelial proliferation, fibroblast proliferation, synthesis and deposition of collagen, production of elastin and proteoglycans, revascularization, and wound contraction. ,, The incorporation of laser as a therapeutic tool in the biomedical field has been investigated since 1960 but, in spite of the numerous studies on the effects of laser therapy, it is difficult to justify physical variables such as application technique, dosages, depth, modes, and duration of exposure.  It has been observed that photostimulation influences the macrophage production of growth factors, which increases cell proliferation. , In 1976, Mester et al.  reported that low-level helium-neon (He-Ne) laser could aid the healing of mechanical injuries. Since then, it was shown that this laser has several effects on living tissue, effects known as laser biostimulation.
Laser photobiomodulation has been increasingly used with the purpose of improving the quality of wound healing.  The therapeutic effects of laser on the different biological types are in a broad range and include trophic-regenerative, anti-inflammatory, and analgesic effects. , It has also been demonstrated that tissue regeneration becomes more effective when treated with low-level laser. ,,, There are reports that laser irradiation stimulates the release of fibroblast growth factor and the replication of these cells. , Irradiation with He-Ne laser would accelerate the healing process, with a better weave of collagen fibers ,,, and greater collagen deposition,  combined with faster re-epithelialization and neovascularization. ,
In situations of deficient healing, such as ischemia, diabetes, and pressure ulcers, irradiation with a low-level laser could be an alternative for the recovery. , The objective of the present study was to evaluate the effects of low-level He-Ne laser on the healing process of oral mucosal wounds in rats.
| Materials and methods|| |
The animal experimental program was performed under a licensing agreement with the permission of the animal use committee at the School of Dentistry, Rafsnjan University of Medical Sciences, Rafsanjan, Iran.
Thirty-two 1-2-year-old Wistar male white rats were kept in metal cages at room temperature with 12 h of light per day and 37% relative humidity. They received a standard laboratory diet and water ad libitum. Before the experimental procedures, the rats were randomly divided into four groups of eight rats each. After weighing, each animal received an anesthetic injection of 10% ketamine (80 mg/kg) and 1% acepromazine (2.5 mg/kg). After anesthesia, the intraoral surgical field together with the hand piece and fiber of the laser device were sterilized with Betadine solution (Behsa, Arak, Iran). Two parallel incisions by steel scalpel (Bard-Parker number 15) and approximately 10 mm in length were performed in the buccal mucosa of each rat. The buccal mucosa was selected for the oral wounds because of its accessibility. The incisions were not sutured. When we were used laser radiation for each incision, we were covered other incision on the buccal cavity by the steel spatula. The device laser He-Ne Plasmax IV, LHN 9709 (KLD Biossistemas® ) was used. The wounds were treated with He-Ne laser at energy density of 1 J/cm 2 , the maximal continuous energy level of 5 mW, with the wavelength of 632.8 nm and laser beam area of 0.2 cm 2 that was employed for 40 s. The laser tip beam was kept perpendicular to the irradiated tissue surface. Laser radiation was used just in a 1 st day, 1 st and 2 nd day, 1 st and 3 rd day, and continuous 3 days in groups of A, B, C, and D of rats, respectively. All of the surgical procedures were performed by the same operator under aseptic conditions. The rats were then returned to their cages, without limitations of activity. To prevent postsurgical infections, antibiotics (penicillin 0.5 mL intramuscular) were administered on the day of the surgical procedure.
The rats were killed by euthanasia (high concentration of CO 2 in air) at intervals of 5 days after the surgical procedure for four groups. Specimens measuring approximately 5 mm × 10 mm were then removed from the control (left) and test (right) sides of each animal. The histological sections were stained with hematoxylin-eosin (H and E) by standard procedures. Briefly, a sample was excised from the mucosal bed of each mouse and was fixed in buffered formalin at 10% for 24 h and later submitted to the routine histological procedure. Through the H and E staining, the general morphological evaluation of the wound was obtained, and the inflammatory pattern was recognized. Ten fields were viewed at ×400 magnification, according to the guidelines described by Vizzotto.  For the cell count, the following scale was adopted: No cell = 1; up to 50 cells = 2; 50-100 cells = 3 and more than 100 cells = 4, positive for monomorphonuclear cells and negative for polymorphonuclear cells After the attribution of the indices, their total was calculated so that each group of animals had a final score for classification into three phases of the inflammatory process. 
Histological results were displayed as healing grade and processed for Wilcoxon analysis. Kruskal-Wallis tests were used to determine the effect of the groups and the evaluation days on the study variables. A probability of a null hypothesis of <5% (P < 0.05) was regarded as statistically significant.
| Results|| |
Healing grades in laser-irradiated site in four groups of rats were as follow: A=2, B=2, C=3 and D=4 while these grades in no laser-irradiated site were A=1, B=2, C=3 and D=3. Histological analysis showed that the tissue healing after continuous 3 days on the laser irradiated side was better than the control side, but there was no difference between the two sides in each group. The difference between all groups was not statistically significant (P > 0.05).
While the number of inflammatory cells in laser radiation group was lower than the control [Figure 1] and [Figure 2] and plumped fibroblast [Figure 3] were very evident in the laser radiated specimens (P < 0.05), but there was no significant differences between laser radiated and control group in each divided rats (P > 0.05).
|Figure 1: Laser therapy after 5 days in the nonradiated group showing smaller fibroblast and more inflammatory cells.|
Click here to view
|Figure 2: Laser therapy after 5 days in radiated group showing a decreasing number of inflammatory cells and enlarged fibroblasts.|
Click here to view
| Discussion|| |
Open wounds have lost the barrier that protects tissues from bacterial invasion and allow for the escape of vital fluids. Wound healing and tissue repair are complex processes that involve a dynamic series of events including clotting, inflammation, granulation tissue formation, epithelization, collagen synthesis, and tissue remodeling. 
In recent years, low-intensity laser therapy has gained considerable recognition and importance among treatment modalities for various medical problems including wound repair processes, musculo-skeletal complications, and pain control. ,,, Clinical studies have shown low energy lasers to be effective as analgesics and to accelerate the healing of injured tissue. 
Several studies have been done on low-level laser therapy (LLLT); however, conflicting results and little research on the mouth was an incentive for us to do this research. A Ne-He laser was used in this study, and the results showed those 5 days after the therapy, healing was better on the side receiving laser radiation. This result was similar to the results of some previous studies, ,,, although the results were in contrast to many other studies. , In the previous studies, researchers believed that the differences between fluency energy level in tissues, , frequency of radiation,  systemic effect,  and the type of ulcer  would influence the results of low level laser exposures and generate conflicting results.
Ghalayani et al.  assessment the effect of diode laser therapy on incisional wound healing and expression of iNOS and eNOS on rat oral tissue. Results showed that the tissue healing after 7 days on the laser-irradiated side was better than the control side, but there was no significant difference between the two sides on the days 2, 14, and 21 after surgery. The difference in the amounts of iNOS between the groups was significant; it was more in the laser-irradiated side than the control side. 
Vinck et al.  carried out a cell culture study to observe the influence of diode light emission and LLLT in the process of wound healing, in which cultures of fibroblasts obtained from 8-day-old chicken embryos were treated for 3 consecutive days. Infrared laser gallium-aluminum arsenate was applied with three wavelengths emitted separately. All three applications covered an area of 18 cm 2 and used a frequency of 0-1, 500 Hz, at an application distance of 0.6 cm. LLLT was used with the following parameters: One 5 s emission with a peak potency of 40 mW resulting in an exposure of 1 J/cm 2 . Infrared, the light spectrum with red light, has a radiation exposure of 0.53 J/cm 2 . Green light emits 0.1 Jcm 2 , corresponding to an exposure time of 1, 2, and 3 min, respectively, with peak potencies equivalent to 160, 80, and 10 mW, respectively. Statistical analysis showed a high proliferation of fibroblasts in vitro. Therefore, this study presupposed the possibility of stimulatory effects on in vivo wound healing when treated with LLLT. Demir et al.  investigated the effects of electrical stimulation (ES) and LLLT in wound healing in an experimental study using Swiss-albino female rats which were divided into four groups of 30 animals each. A 6 cm linear incision was made in the dorsal region of each rat. Results showed ES and laser treatments have positive effects on inflammation, proliferation, and maturation of wounds and can be successfully used for decubitus ulcers and chronic wounds.
An experimental study conducted by Khadra  observed the effects of gallium-aluminum arsenate lasers, a diode laser, on bone healing process in 20 rats with 2.7 mm diameter bone defects in the parietal region treated for 4 weeks. On the 28 th day, the animals were euthanized for histological assessment of bone defects. There was a significant increase in calcium, phosphorus, and protein. Similarly, histological analyses showed a marked growth of angiogenesis and connective tissue. They concluded, therefore, that LLLT can favor bone formation in rats with bone defects.
In the present study, the test of choice for assessing the healing grade was histological observation. The healing was compared in different times after the laser therapy. In Group D (3 continuous days of laser therapy), there was histologically better healing on the irradiated side compared to the control side. Improvement in the healing in Group D compared to the other groups on the laser irradiated side could be due to the fact that in the other groups, the rats did not receive enough laser radiation and according to the high rate of repair in rats, there was complete healing in Groups C and D, and there was no difference between the groups. This may explain that use of laser in contentious days was noticed and increase the proliferative rate of fibroblast. However, other studies should be done in human samples that the amount of radiation, radiation time, area to be determined. This study was consistent with the researches of Parirokh, et al.,  and Rezende, et al. 
Results showed that fibroblast proliferation was significantly more evident in the laser radiated than the control group in 5 days interval which was in agreement with previous studies in which low power laser beneficial effects were demonstrated. , To ensure the effects of low-level laser therapy on the healing process, there is still a need for consensus on the standards for the physical variables: Application times and techniques, energy densities, output powers, and wavelengths. The comparison of the results of several authors has been hindered by their use of different methodologies. The use of He-Ne laser (632.8 nm), applied with different densities can lead to different cellular responses, and this may preclude comparisons. Hawkins and Abrahamse  applied doses of 0.5, 2.5, 5, 10, and 16 J/cm 2 to human skin fibroblasts on 2 consecutive days and found that 5 J/cm 2 stimulated mitochondrial activity, cell proliferation, and fibroblast migration. However, higher doses decreased cell viability and proliferation and damaged the cell membrane and DNA. 
| Conclusion|| |
This study showed that He-Ne laser has had beneficial effects on incisional wound healing. However this result is not significant statistically. More research with the high sample on acute and chronic ulcers is recommended.
The presented study was conducted in accordance with Helsinki Declaration (1974) and recommendation of the college of Veterinary, Kerman University, Kerman, Iran, an entity as associated with the International Council for Laboratory Animal Sciences. This project was approved by the Animal Research Ethics Committee of Rafsanjan University of Medical Sciences under the protocol number 418.
Financial support and sponsorship
Conflicts of interest
The authors of this manuscript declare that they have no conflicts of interest, real or perceived, financial or non-financial in this article.
| References|| |
Liao X, Xie GH, Liu HW, Cheng B, Li SH, Xie S, et al.
Helium-neon laser irradiation promotes the proliferation and migration of human epidermal stem cells in vitro
: Proposed mechanism for enhanced wound re-epithelialization. Photomed Laser Surg 2014;32:219-25.
Hewitson TD. Renal tubulointerstitial fibrosis: Common but never simple. Am J Physiol Renal Physiol 2009;296:F1239-44.
Parirokh M, Dabiri S, Bahrampour A, Homayon Zadeh M, Eghbal MJ. Effect of low power laser on incisional wound healing. Iran Endod J 2006;1:45-8.
Busnardo VL, Biondo-Simões ML. Effects of low-level helium-neon laser on induced wound healing in rats. Rev Bras Fisioter 2010;14:45-51.
Carvalho Pde T, Silva IS, Reis FA, Perreira DM, Aydos RD. Influence of ingaalp laser (660nm) on the healing of skin wounds in diabetic rats. Acta Cir Bras 2010;25:71-9.
Gao X, Xing D. Molecular mechanisms of cell proliferation induced by low power laser irradiation. J Biomed Sci 2009;16:4.
Mester E, Nagylucskay S, Döklen A, Tisza S. Laser stimulation of wound healing. Acta Chir Acad Sci Hung 1976;17:49-55.
Adamskaya N, Dungel P, Mittermayr R, Hartinger J, Feichtinger G, Wassermann K, et al.
Light therapy by blue LED improves wound healing in an excision model in rats. Injury 2011;42:917-21.
Freitas CP, Melo C, Alexandrino AM, Noites A. Efficacy of low-level laser therapy on scar tissue. J Cosmet Laser Ther 2013; 15:171-6.
Hashmi JT, Huang YY, Osmani BZ, Sharma SK, Naeser MA, Hamblin MR. Role of low-level laser therapy in neurorehabilitation. PM R 2010;2 12 Suppl 2:S292-305.
Hashmi JT, Huang YY, Sharma SK, Kurup DB, De Taboada L, Carroll JD, et al.
Effect of pulsing in low-level light therapy. Lasers Surg Med 2010;42:450-66.
Schmidt CE, Leach JB. Neural tissue engineering: Strategies for repair and regeneration. Annu Rev Biomed Eng 2003;5:293-347.
AboElsaad NS, Soory M, Gadalla LM, Ragab LI, Dunne S, Zalata KR, et al.
Effect of soft laser and bioactive glass on bone regeneration in the treatment of infra-bony defects (a clinical study). Lasers Med Sci 2009;24:387-95.
Bao P, Kodra A, Tomic-Canic M, Golinko MS, Ehrlich HP, Brem H. The role of vascular endothelial growth factor in wound healing. J Surg Res 2009;153:347-58.
Khoo NK, Shokrgozar MA, Kashani IR, Amanzadeh A, Mostafavi E, Sanati H, et al. In vitro
Therapeutic effects of low level laser at mRNA level on the release of skin growth factors from fibroblasts in diabetic mice. Avicenna J Med Biotechnol 2014;6:113-8.
Carvalho PT, Mazzer N, dos Reis FA, Belchior AC, Silva IS. Analysis of the influence of low-power HeNe laser on the healing of skin wounds in diabetic and non-diabetic rats. Acta Cir Bras 2006;21:177-83.
de Lima FJ, Barbosa FT, de Sousa-Rodrigues CF. Use alone or in combination of red and infrared laser in skin wounds. J Lasers Med Sci 2014;5:51-7.
Reis SR, Medrado AP, Marchionni AM, Figueira C, Fracassi LD, Knop LA. Effect of 670-nm laser therapy and dexamethasone on tissue repair: A histological and ultrastructural study. Photomed Laser Surg 2008;26:307-13.
Corazza AV, Jorge J, Kurachi C, Bagnato VS. Photobiomodulation on the angiogenesis of skin wounds in rats using different light sources. Photomed Laser Surg 2007;25:102-6.
de Araújo CE, Ribeiro MS, Favaro R, Zezell DM, Zorn TM. Ultrastructural and autoradiographical analysis show a faster skin repair in He-Ne laser-treated wounds. J Photochem Photobiol B 2007;86:87-96.
Marques CR, Martin AA, Lima CJ, Conrado LA, Silveira FL, Carvalho MV. The use of hyperbaric oxygen therapy and LED therapy in diabetic foot. Biomedical Optics 2004 2004; 5312:47-53.
Dabiri G, Heiner D, Falanga V. The emerging use of bone marrow-derived mesenchymal stem cells in the treatment of human chronic wounds. Expert Opin Emerg Drugs 2013; 18:405-19.
Vizzotto O. Effect of the cisplatin applied before and after the surgical procedure on the healing of colonic anastomoses in rats. J Bras Patol Med Lab 2003;39:143-9.
Reddy GK, Stehno-Bittel L, Enwemeka CS. Laser photostimulation accelerates wound healing in diabetic rats. Wound Repair Regen 2001;9:248-55.
Maiya GA, Kumar P, Rao L. Effect of low-intensity helium-neon (He-Ne) laser irradiation on diabetic wound healing dynamics. Photomed Laser Surg 2005;23:187-90.
Abtahi SM, Mousavi SA, Shafaee H, Tanbakuchi B. Effect of low-level laser therapy on dental pain induced by separator force in orthodontic treatment. Dent Res J (Isfahan) 2013;10:647-51.
Seifi M, Atri F, Yazdani MM. Effects of low-level laser therapy on orthodontic tooth movement and root resorption after artificial socket preservation. Dent Res J (Isfahan) 2014;11:61-6.
Khozeimeh F, Moghareabed A, Allameh M, Baradaran S. Comparative evaluation of low-level laser and systemic steroid therapy in adjuvant-enhanced arthritis of rat temporomandibular joint: A histological study. Dent Res J (Isfahan) 2015;12:215-23.
Hawkins D, Houreld N, Abrahamse H. Low-level laser therapy (LLLT) as an effective therapeutic modality for delayed wound healing. Ann N Y Acad Sci 2005;1056:486-93.
Saperia D, Glassberg E, Lyons RF, Abergel RP, Baneux P, Castel JC, et al.
Demonstration of elevated type I and type III procollagen mRNA levels in cutaneous wounds treated with helium-neon laser. Proposed mechanism for enhanced wound healing. Biochem Biophys Res Commun 1986;138:1123-8.
Hussein AJ, Alfars AA, Falih MA, Hassan AN. Effects of a low level laser on the acceleration of wound healing in rabbits. N Am J Med Sci 2011;3:193-7.
Allendorf JD, Bessler M, Huang J, Kayton ML, Laird D, Nowygrod R, et al.
Helium-neon laser irradiation at fluences of 1, 2, and 4 J/cm2 failed to accelerate wound healing as assessed by both wound contracture rate and tensile strength. Lasers Surg Med 1997;20:340-5.
Neiburger EJ. Accelerated healing of gingival incisions by the helium-neon diode laser: A preliminary study. Gen Dent 1997;45:166-70.
In de Braekt MM, van Alphen FA, Kuijpers-Jagtman AM, Maltha JC. Effect of low level laser therapy on wound healing after palatal surgery in beagle dogs. Lasers Surg Med 1991;11:462-70.
Ghalayani P, Jahanshahi G, Birang R, Bazazzadeh M. Assessment of the effect of diode laser therapy on incisional wound healing and expression of iNOS and eNOS on rat oral tissue. Dent Res J (Isfahan) 2013;10:348-52.
Isman E, Aras MH, Cengiz B, Bayraktar R, Yolcu U, Topcuoglu T, et al.
Effects of laser irradiation at different wavelengths (660, 810, 980, and 1064 nm) on transient receptor potential melastatin channels in an animal model of wound healing. Lasers Med Sci 2015;30:1489-95.
Vinck EM, Cagnie BJ, Cornelissen MJ, Declercq HA, Cambier DC. Increased fibroblast proliferation induced by light emitting diode and low power laser irradiation. Lasers Med Sci 2003;18:95-9.
Demir H, Balay H, Kirnap M. A comparative study of the effects of electrical stimulation and laser treatment on experimental wound healing in rats. J Rehabil Res Dev 2004;41:147-54.
Khadra M, Kasem N, Haanaes HR, Ellingsen JE, Lyngstadaas SP. Enhancement of bone formation in rat calvarial bone defects using low-level laser therapy. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2004;97:693-700.
Rezende SB, Ribeiro MS, Núñez SC, Garcia VG, Maldonado EP. Effects of a single near-infrared laser treatment on cutaneous wound healing: Biometrical and histological study in rats. J Photochem Photobiol B 2007;87:145-53.
Karu T. Photobiology of low-power laser effects. Health Phys 1989;56:691-704.
Hawkins DH, Abrahamse H. The role of laser fluence in cell viability, proliferation, and membrane integrity of wounded human skin fibroblasts following helium-neon laser irradiation. Lasers Surg Med 2006;38:74-83.
[Figure 1], [Figure 2], [Figure 3]
|This article has been cited by|
||Effectiveness of an 810-nm Diode Laser in Addition to Non-surgical Periodontal Therapy in Patients With Chronic Periodontitis: A Randomized Single-Blind Clinical Trial
| ||Majid Reza Mokhtari,Farzaneh Ahrari,Shokoufeh Dokouhaki,Amir Fallahrastegar,Alireza Ghasemzadeh |
| ||Journal of Lasers in Medical Sciences. 2021; 12(1): e37 |
|[Pubmed] | [DOI]|
||Effectiveness of Low-Level Laser Irradiation in Reducing Pain and Accelerating Socket Healing After Undisturbed Tooth Extraction
| ||Farzaneh Ahrari,Majid Eshghpour,Reza Zare,Samaneh Ebrahimi,Amir Fallahrastegar,Hossein Khaki |
| ||Journal of Lasers in Medical Sciences. 2020; 11(3): 274 |
|[Pubmed] | [DOI]|
||Effectiveness of low-level laser therapy on recovery from neurosensory disturbance after sagittal split ramus osteotomy: a systematic review and meta-analysis
| ||Parsa Firoozi,Seied Omid Keyhan,Seong-Gon Kim,Hamid Reza Fallahi |
| ||Maxillofacial Plastic and Reconstructive Surgery. 2020; 42(1) |
|[Pubmed] | [DOI]|
||Wound Photobiomodulation Treatment Outcomes in Animal Models
| ||Alondra Lopez,Cord Brundage |
| ||Journal of Veterinary Medicine. 2019; 2019: 1 |
|[Pubmed] | [DOI]|
||A randomized clinical trial comparing the efficacy of low-level laser therapy (LLLT) and laser acupuncture therapy (LAT) in patients with temporomandibular disorders
| ||Azamsadat Madani,Farzaneh Ahrari,Amir Fallahrastegar,Naeemeh Daghestani |
| ||Lasers in Medical Science. 2019; |
|[Pubmed] | [DOI]|
||Acute compartment syndrome of the leg due to infection following an insect bite
| ||Jianzhang Wang,Qiang Duan,Xiaolong Sun,Xiang Mou,Baoqiang Song,Hua Yuan |
| ||Medicine. 2018; 97(30): e11613 |
|[Pubmed] | [DOI]|
||Effect of low-level laser on diabetic oral wound healing
| ||Mohamed M. Osman,Khaled Hassan |
| ||Egyptian Journal of Oral & Maxillofacial Surgery. 2017; 8(1): 8 |
|[Pubmed] | [DOI]|
||Is low level laser therapy effective for treatment of neurosensory deficits arising from sagittal split ramus osteotomy?
| ||Majid Eshghpour,Baratollah Shaban,Farzaneh Ahrari,Mahdi Erfanian,Elaheh Shadkam |
| ||Journal of Oral and Maxillofacial Surgery. 2017; |
|[Pubmed] | [DOI]|
||Role of laser irradiation in direct pulp capping procedures: a systematic review and meta-analysis
| ||Fawad Javed,Sergio Varela Kellesarian,Tariq Abduljabbar,Elham Gholamiazizi,Changyong Feng,Khaled Aldosary,Fahim Vohra,Georgios E. Romanos |
| ||Lasers in Medical Science. 2016; |
|[Pubmed] | [DOI]|