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Review Article:
Evaluation of the effect of antimicrobial nanoparticles on bond strength of orthodontic adhesives: A review article
Amirhossein Mirhashemi, Leila Jazi
Dent Res J
2021, 18:110 (10 December 2021)
DOI
:10.4103/1735-3327.332104
Background:
Antimicrobial nanoparticles (NPs) have various applications in different fields of dentistry. The purpose of incorporating NPs into orthodontic adhesives is to inhibit the cariogenic bacteria and reduce decalcifications around bonded orthodontic brackets. However, they may affect the physical and mechanical properties of adhesive such as shear bond strength (SBS). This review was done to answer the question whether the incorporation of antimicrobial NPs into orthodontic adhesives changes the SBS.
Materials and Methods:
An electronic search was performed with keywords such as adhesives AND nanoparticles AND orthodontics AND shear strength. After screening and applying eligibility criteria, 18 relevant studies were included.
Results:
The pooled data suggest that except for 10 wt% of various NPs incorporation, there is no significant difference in SBS between control conventional adhesives and experimental modified ones with tested concentrations.
Conclusion:
The SBS of orthodontic adhesives containing up to 5% NPs is in clinical acceptable range. However, generalizing the results to
in vivo
situation may be problematic and further studies are required.
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Original Article:
Comparison of dental treatments performed under general anesthesia for healthy and disabled children
Nasser Kaviani, Maryam Ghafournia, Salim Mirzaali, Shirin Marzoughi, Reza Salari-Moghaddam
Dent Res J
2021, 18:109 (10 December 2021)
DOI
:10.4103/1735-3327.332103
Background:
This study aimed to assess and compare the type of dental procedures performed under general anesthesia for healthy and disabled children.
Materials
and Methods:
This descriptive, cross-sectional study evaluated 361 dental records of children who received dental treatments under general anesthesia in the operating room of Torabinejad Research Center during 2011–2013. Patients with mental or physical disability were categorized as disabled. The age and gender of patients, number of treated teeth, duration of general anesthesia, type of tooth, and type of dental treatment such as extraction, pulp therapy, placement of stainless steel crowns, composite restoration, preventive resin restoration (PRR), fissure sealant treatment, and fluoride therapy were separately recorded for the healthy group and patients with disability. Data were analyzed using one-way ANOVA, and independent sample
t
-test at
P
< 0.05 level of significance.
Results:
Of 361 patients, 263 patients were healthy and 102 patients had disability. Of all disabled children, 48% had physical and 52% had mental disability. Among patients with physical disability, allergy (40%), followed by cardiovascular diseases (26%) were the most common. Mental retardation (54%) followed by cerebral palsy (10%) were the most common mental disabilities. Number of extracted teeth was significantly higher in disabled children (
P
= 0.006). Furthermore, disabled children received significantly lower PRR (
P
= 0.015), fissure sealant treatment (
P
= 0.003), fluoride therapy (
P
= 0.002), and pulp therapy (
P
< 0.001) compared with healthy children.
Conclusion:
Tooth extraction has a higher frequency in disabled children; while, attempts are made to preserve the teeth as much as possible in healthy children.
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Original Article:
Evaluation of stress distribution of porous tantalum and solid titanium implant-assisted overdenture in the mandible: A finite element study
Ayshin Akbarzadeh, Yasser Hemmati, Fariba Saleh-Saber
Dent Res J
2021, 18:108 (10 December 2021)
DOI
:10.4103/1735-3327.332102
Aims:
The present study aimed to evaluate the stress distribution of porous tantalum implant and titanium solid implant assisted overdenture (IAO) in mandibular bone by utilizing three-dimensional (3D) finite element (FE) analysis.
Materials and Methods:
In this FE study, an existing cone-beam volumetric tomography scan of a patient without any maxillofacial anomaly with an available acceptable IAO for mandible was used to attain the compartments of a completely edentulous mandible. Zimmer trabecular implants and locator attachment systems were selected as the case group (Model B), and Zimmer Screw-Vent implants and locator attachment system were chosen for the control (Model A), as overdenture attachments in the present study. The mandibular overdenture was scanned and digitized as a FE model. Two 3D FE models were designed as edentulous lower jaws, each with four implants in the anterior section of the mandible. Three forms of loads were directed to the IAO in each model: Vertical loads on the left first molar vertical molar (VM). Vertical loads on the lower incisors (VI). Inclined force buccolingually applied at left first molar (IM).
Results:
Under all loading conditions, the maximum strain values in peri-implant bone in Model A were less than Model B. Under VI, the greatest stress value around abutments in both models was about 2–3 times higher than the other loads. Under VM and IM loads, no significant difference was observed between models.
Conclusion:
Using trabecular metal implants instead of solid implants reduces strain values around both cortical and trabecular bone.
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Original Article:
Comparison of the cleaning efficacy of XP-endo shaper and Mtwo rotary files in oval-shaped canals
Narges Simdar, Nazanin Bashardoust, Majid Jahangir
Dent Res J
2021, 18:107 (10 December 2021)
DOI
:10.4103/1735-3327.332101
Background:
Cleaning and shaping of root canals are essential steps for the success of endodontic therapy. This study compared two types of rotary files in oval-shaped root canals: XP-endo shaper (FKG, La Chaux-de- Fonds, Switzerland) and Mtwo (VDW, Germany, Munich) with regard to cleaning ability and canal preparation. Mtwo is a system of nickel–titanium files with S-shaped cross-sectional design and XP-endo shaper can change its shape according to the temperature.
Materials and Methods:
This
in vitro
study was performed on 16 pairs of freshly extracted contralateral mandibular premolars with a single oval-shaped canal that were selected and divided into two groups according to the root canal instrumentation technique: XP-endo shaper and Mtwo. Then, each root cut into three coronal, middle and apical sections and processed for histologic evaluation of canal wall planning and the presence of debris. Sections were evaluated by using AutoCAD 2017 software. Statistical analysis was used to compare between both the groups using repeated measures multivariate analysis of variance with Bonferroni correction for
post hoc
comparison and independent sample
t
-tests. The level of statistical significance was set at
P
< 0.05.
Results:
With a statistically significant difference in the middle third, untouched area and area with debris in XP-endo shaper group were smaller (respectively
P
= 0.013 and
P
= 0.011). Despite the percentage difference between groups, there was not a statistically significant difference in other sections.
Conclusion:
Statistically in the middle section of the oval-shaped canals, the XP-endo shaper performs better than the Mtwo rotary files.
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Original Article:
Effect of whitening toothpastes containing activated charcoal, abrasive particles, or hydrogen peroxide on the color of aged microhybrid composite
Pouya Rostamzadeh, Ladan Ranjbar Omrani, Mahdi Abbasi, Mir Saeed Yekaninejad, Elham Ahmadi
Dent Res J
2021, 18:106 (10 December 2021)
DOI
:10.4103/1735-3327.332100
Background:
This study aimed to assess the effect of different whitening toothpastes containing activated charcoal, abrasive particles or hydrogen peroxide on the color of aged microhybrid composite.
Materials
and Methods:
In this
in vitro
, experimental study, 45 composite discs (2 mm × 7 mm) were fabricated of a microhybrid composite. They underwent accelerated artificial aging for 300 h, corresponding to 1 year of clinical service. The composites were then randomly divided into five groups (
n
= 9). One group served as the control and underwent tooth brushing with distilled water. The remaining four groups underwent tooth brushing with Colgate Total whitening (Gt), Colgate Optic White (Go), Perfect White Black (Gp) and Bencer (Gb) toothpastes in a brushing machine The International Commission on Illumination values (L
m
, a
m
, b
m
) were determined using a spectrophotometer. Color change (ΔE) calculated based on this formula: ΔE
m
= ([ΔL
m
]
2
+ [Δa
m
]
2
+ [Δb
m
]
2
)
½
. The differences were defined by ΔE
1
(after aging-baseline),ΔE
2
(after brushing-after aging) and ΔE
3
(after brushing-base line). ΔE
1
were evaluated to ensure that color mismatch had occurred (△E1 > 5.5). Difference in (L, a, b) parameters after aging and after tooth brushing in each group, color parameter changes (ΔL
2,
Δa
2
, Δb
2,
ΔL
3,
Δa
3
, Δb
3
) and ΔE
2
and ΔE
3
were analyzed and compared using Wilcoxon test and independent sample median test at
P
= 0.05 level of significance.
Results:
The color parameter changes, ΔE
3
and △ E
2
were not significantly different among the five groups (
P
> 0.05). In Gp and Gb charcoal a*, b*, and L* after tooth brushing (
P
< 0.05). In Colgate Optic group, the a* parameter significantly decreased while the L* parameter significantly increased (
P
< 0.05).
Conclusion:
The results showed that there is no significant difference in the color change of Spectrum composite following tooth brushing with different whitening toothpastes for two weeks. It should be noted that △ E
3
reached to <3.3 only in charcoal whitening toothpastes.
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Original Article:
Autistic children's parents and hospital dentistry
Dana Tahririan, Saharosadat Shariati, Firoozeh Nilchian
Dent Res J
2021, 18:105 (10 December 2021)
DOI
:10.4103/1735-3327.332099
Background:
It is difficult to perform dental procedures in autistic children, and parental involvement is necessary for successful hospital dental services. Therefore, in order to promote oral health in autistic children, this study was aimed to explore the knowledge, attitude, and performance of autistic children's parents with respect to hospital dentistry.
Materials
and Methods:
This cross-sectional study was conducted with the parents of 100 autistic children aged 2–6 years selected from among the children of Isfahan autism treatment centers. A self-administered questionnaire, including parental demographic information and 22 items on the assessment of knowledge, attitude, and performance of autistic children's parents regarding hospital dental procedures under general anesthesia, was completed by 100 parents.
P
<0.05 was considered statistically significant. Data were analyzed by SPSS software using Chi-square test.
Results:
A total of 100 parents of autistic children, with an average age of 37.4 ± 6.1 years, were recruited in this study. The results showed that 56%, 50%, and 3% of parents had poor knowledge about dental hospital services, dental complications, and hospital dentistry rules, respectively. Further, 51% of parents believed that general anesthesia was dangerous to their children. In addition, 69% of children had little or no cooperation with the dentist. There was also a significant relationship between the knowledge, attitude, and performance of autistic children's parents regarding hospital dentistry and the parents' age and sex.
Conclusion:
This study showed that autistic children's parents had poor knowledge, attitude, and performance with respect to hospital dentistry.
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Original Article:
Frequency of caries in triangular-shaped radiolucencies on periapical radiographs of maxillary deciduous second molars
Mitra Tabari, Mina Yazdizadeh, Farida Abesi, Soraya Khafri, Javad Vaziri Dozin
Dent Res J
2021, 18:104 (10 December 2021)
DOI
:10.4103/1735-3327.332098
Background:
Dentists might face various artifacts (such as triangular-shaped radiolucencies [TSRs]) during the assessment of radiographs and should be able to differentiate them from caries to avoid unnecessary treatments.
Materials
and Methods:
In this cross-sectional study, 109 maxillary second primary molars were evaluated in cooperative children aged 4–9 years, who had distal caries in their maxillary first primary molars. First, TSRs were recorded on periapical radiographs of each maxillary second primary molar's proximal surface. Then, after excavating distal caries in the adjacent teeth “D,” a pedodontist examined the mesial surfaces of teeth “E.” Chi-square test was used to compare the distribution of caries in different variables, and the kappa coefficient was applied to evaluate clinical and radiographic agreements. A
P
< 0.05 was considered statistically significant.
Results:
Forty-four cases were found to be carious both clinically and radiographically, and 54 cases were noncarious by both methods, while for 11 cases, the diagnosis was controversial. No statistically significant difference was found between radiographic and clinical caries detection methods in children whose periapical radiographs contained TSRs, and most of the subjects had similar diagnoses. Value of caries detection sensitivity, specificity, positive predictive value, and negative predictive value in TSRs was 88%, 92%, 90%, and 90%, respectively.
Conclusion:
Considering high radiographic sensitivity for caries detection in TSRs, clinicians should be more cautious about them being carious or not, and both radiographic and clinical examinations are necessary. Further, to avoid misinterpretation in radiographs, additional education is necessary for young dentists.
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Letter To Editor:
Publication during COVID-19, introducing useful resources for research
Shahroo Etemad-Moghadam, Mojgan Alaeddini
Dent Res J
2021, 18:103 (10 December 2021)
DOI
:10.4103/1735-3327.332097
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Case Report:
Juvenile trabecular ossifying fibroma of the maxilla: Case report of a diagnostic dilemma
Hassan Mir Mohammad Sadeghi, Zahra Sadat Torabi, Hamidreza Moslemi, Shervin Shafiei, Sanaz Gholami Toghchi, Fatemeh Mashhadiabbas
Dent Res J
2021, 18:102 (10 December 2021)
DOI
:10.4103/1735-3327.332096
Ossifying fibromas (OFs) are benign, well-demarcated lesions in the craniofacial region, particularly in the jaws, with clinical, radiographic, and histopathological similarities to other lesions, which make their diagnosis challenging. Herein, we report a case of a fibro-osseous lesion in the anterior maxilla of a 13-year-old boy, consisting of an intraosseous and an extra-osseous part, which created a diagnostic dilemma.
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Original Article:
How the initial retentive force of implant-supported overdentures can be affected with splinted and unsplinted attachments systems
Farahnaz Nejatidanesh, Omid Savabi, Ghazal Savabi, Mehdi Razavi
Dent Res J
2021, 18:101 (10 December 2021)
DOI
:10.4103/1735-3327.332095
Background:
The aim of this study was to evaluate the retention of implant-supported overdentures with different attachment systems.
Materials and Methods:
In this
in vitro
study edentulous model with 2 Straumann implant in symphyseal region was used to make an overdenture with different attachment systems. (Dolder bar with 1 and 3 metal clips, Hader bar with 1 and 3 plastic clips, ball on bar with 2 and 4 plastic caps, Locator, Rhein plastic caps and Eleptical matrix). Retention values were recorded by universal testing machine with a cross speed of 50.8 mm/min in vertical, posteroanterior, and lateral direction. Repeated measure ANOVA and Duncan tests were used for the data analysis (α =0.05).
Results:
There was a statistically significant difference between the retention values of studied attachments in different dislodgment directions (
P
< 0.05). The highest and lowest retention were recorded for 4 balls on bar (56.71 N) and Rhein pink caps (27.89 N) in the vertical direction. Three metal clips (61.43 N) and Rhein pink cap (24.77 had the highest and lowest retention force in the posteroanterior direction. In the lateral direction, 4 balls on bar (62.68 N) and 1 plastic clip (32.27 N) showed the highest and lowest retention, respectively.
Conclusion:
If the higher retention force has been considered for implant-supported overdenture attachment selection, the clinician can use splinted bar or ball on bar superstructure.
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Original Article:
The clinical and radiographic evaluation of Allium sativum oil (garlic oil) in comparison with mineral trioxide aggregate in primary molar pulpotomy
Taraneh Faghihi, Roza Haghgoo, Fateme Taghizade, Mehrshad Zareiyan, Majid Mehran, Ghassem Ansari
Dent Res J
2021, 18:100 (22 November 2021)
DOI
:10.4103/1735-3327.330881
Background:
Mineral trioxide aggregate (MTA) is known as a common biomaterial for pulpotomy of primary molars, with limitation including high cost, difficult handling, and long setting time. This study was conducted to compare the clinical and radiographic success rate of Allium sativum oil and MTA in pulpotomy of primary molar teeth.
Materials and Methods:
This randomized, controlled, clinical trial was performed on a group of children with at least two symptom-free primary molars requiring pulpotomy. A total of 90 teeth were randomly assigned into two groups: After pulp amputation and hemostasis, Group I received MTA as medication in the pulp chamber and Group II received Allium sativum oil over the pulp stumps. Pulp chamber was then filled with reinforced ZOE paste (zinc oxide eugenol) and teeth were restored using stainless steel crowns. The follow-up scheme of 6, 12, and 18 months was set, and teeth evaluation was conducted by a calibrated pediatric dentist based on the modified criteria proposed by Zurn and Seale. The results were analyzed using the generalized estimating equation analysis with the
P
= 0.05 as the level of significance.
Results:
The clinical success rate was 100% in both groups after all follow-ups. The radiographic success rate was, however, 91.1% after 6.75. 6% after 12 and 18 months in Group II while it was 95.6% after 6, 91.1% after 12 and 18 months in Group I in those follow-up points. The difference between the two groups was not statistically significant.
Conclusion:
According to the results, Allium sativum oil has a high clinical and radiographic success rate comparable to those of MTA.
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Original Article:
Effect of aging on color stability of amine-free resin cement through the ceramic laminate veneer
Keyvan Saati, Sara Valizadeh, Saeed Nemati Anaraki, Niloufar Moosavi
Dent Res J
2021, 18:99 (22 November 2021)
DOI
:10.4103/1735-3327.330880
Background:
This study aimed to assess the color change of two amine-free dual-cure resin cements following aging in comparison with a light-cure resin cement.
Materials and Methods:
This
in vitro
, experimental study evaluated 6 groups (
n
= 10), including three groups of cements bonded to feldspathic porcelain and three groups of resin cements alone. Panavia V5 (Kuraray) and G-CEM LinkForce (GC) dual-cure resin cements and Choice 2 (Bisco) light-cure resin cements were bonded to porcelain according to the manufacturers' instructions. The color parameters of the groups were measured before and after 5000 thermal cycles by spectrophotometry. Data were analyzed using two-way ANOVA and Tukey's
post hoc
test (
α
=0.05).
Results:
The color change (ΔE) of the cement groups alone was significantly greater than that of porcelain-bonded cements. The ΔE of G-CEM LinkForce cement group was significantly higher than that of Panavia V5 group (
P
= 0.020), and the ΔE of the latter group was significantly higher than that of Choice 2 cement group (
P
= 0.021).
Conclusion:
Considering the color change of cements evaluated in this study, Choice 2 and Panavia V5 cements bonded to porcelain showed acceptable color change, which indicates that they are suitable for cementation of esthetic dental restorations. Ceramic laminates have become an ideal esthetic treatment for anterior restorations. The resin cement discoloration can affect final appearance of these restorations. Besides that, exposed resin cement line can lead to the esthetic problem over time.
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Case Report:
Dental management of tricho-dento-osseous syndrome in adolescent patients: Literature review and case presentation
Mojtaba Fazel, Elham Afshari, Neda Jarrahi
Dent Res J
2021, 18:98 (22 November 2021)
DOI
:10.4103/1735-3327.330879
Tricho-dento-osseous syndrome (TDO) is a rare autosomal dominant disorder with complete penetrance. Common clinical features include abnormalities of hair, teeth, and skull. Dental management of TDO patients is quite challenging in terms of existing dental and skeletal problems. The current article presents a 12-year-old girl suffering TDO, followed by a review on the published literature pertaining to the dental management of TDO patients. Patient history included, rejected corneal transplantation, stone-forming kidneys, and several previous dental treatments. She was noted to have signs of mandibular prognatia, frontal bossing of the skull, mild bilateral tibial bowing, microstomia, and labial fissures. Dental findings included severe generalized enamel defects, discolored teeth, microdontia, anterior open-bite, posterior cross-bite, deep periodontal pockets, hyperplastic inflamed gingiva, taurodontism of permanent molars, dental periapical radiolucencies, and missing teeth. She was the only child of healthy, nonconsanguineous parents with no familial history of similar congenital syndrome or dental abnormalities. A treatment plan was established based on medical/dental history and findings, using a team-based approach. This article emphasizes the importance of a multidisciplinary approach for the dental management of patients suffering TDO.
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Randomized Clinical Trial:
The effect of ethanol wet bonding technique on postoperative hypersensitivity of Class II composite restorations: A randomized trial
Alireza Boruziniat, Sanaz Alizadeh, Mahshid Gifani, Zafer C Cehreli, Yeganeh Khazaei
Dent Res J
2021, 18:97 (22 November 2021)
DOI
:10.4103/1735-3327.330878
Background:
To evaluate the effect of Ethanol Wet Bonding Technique (EWBT) on postoperative hypersensitivity (POH) of composite restorations in premolar teeth.
Materials and Methods:
In this randomized trial, 24 patients with at least three proximal carious lesions with similar axial depth and position of gingival floor in their premolars were enrolled. Following cavity preparation, the teeth were randomly assigned to one of three groups: (1) Class II resin-based composite (RBC) restoration using an etch-and-rinse adhesive + wet bonding technique (Control); (2) RBC restoration using EWBT + hydrophobic adhesive; and (3) RBC restoration using EWBT + hydrophilic adhesive. Tooth hypersensitivity was evaluated before and 1, 3, 7, 14 and 30 days after treatment according to the Visual Analog Scale. Data were analyzed statistically with Kruskal–Wallis and Friedman tests (
P
= 0.05).
Results:
All teeth showed similar levels of hypersensitivity after treatment (both
P
> 0.05). Furthermore, there was no difference between POH levels of the test and control groups at any control period (
P
< 0.05). Friedman test indicated that the POH significantly reduced within time in all groups (
P
< 0.05).
Conclusion:
Application of ethanol-wet bonding technique, either with hydrophobic or hydrophilic adhesives did not affect the POH of Class II composite restorations in premolars.
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Original Article:
Effect of curing method and thermocycling on flexural strength and microhardness of a new composite resin with alkaline filler
Mahmoud Bahari, Mehdi Abed Kahnamoui, Mohammad Esmaeel Ebrahimi Chaharom, Soodabeh Kimyai, Zeinab Sattari
Dent Res J
2021, 18:96 (22 November 2021)
DOI
:10.4103/1735-3327.330877
Background:
Cention N has been introduced as an alternative material for amalgam. The purpose was to investigate the flexural strength and microhardness of this material in self-cure and dual-cure modes before and after thermocycling.
Materials and Methods:
In this experimental study, 40 samples of Cention N were prepared in order to determine and compare the microhardness. Half of the samples were set by self-cure method and the other half with dual-cure method. The Vickers microhardness test was performed once after 24 h and again after 10000 thermocyclings. Three-point flexural test was used to determine and compare the flexural strength of 52 rod-shaped samples. Half of the samples were set by self-cure method and the other half with dual-cure method. Among 26 samples in each group, 13 samples were randomly selected and three-point flexural test was performed after 24 h and for another 13 samples after 10,000 thermocyclings. Data were analyzed using two-way ANOVA and paired samples
t
-test (
P
< 0.05).
Results:
There was a statistically significant difference between the mean of microhardness values in two curing methods (
P
< 0.001) and in two storage conditions (
P
< 0.001). The mean of dual-cure microhardness (100.99 ± 7.22) was higher than that of self-cure (64.61 ± 12.51) and the mean value associated with pre-thermocycling (89.75 ± 15.84) was higher than that of the post-thermocycling (76.44 ± 23.56). There was no statistically significant difference between the mean flexural strength in the two curing methods (self-cure [72.85 ± 16.26], dual cure [79.87 ± 23.07]; [
P
> 0.05]). However, the mean flexural strength without thermocycling (85.98 ± 21.74) was higher than that of the thermocycled group (64.24 ± 6.40) (
P
< 0.001).
Conclusion:
The microhardness of Cention N in dual-cure mode was higher than that of self-cure mode, but the flexural strength of dual-cure was not significantly different from that of self-cure. Thermocycling had a significant effect on the microhardness and flexural strength.
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Original Article:
A comparative survival analysis of high viscosity glass ionomer restorations using conventional cavity preparation and atraumatic restorative treatment technique in primary molars: A randomized clinical trial
GY Yunus, Hunny Sharma, Afreen Begum H. Itagi, Himanshu Srivastava
Dent Res J
2021, 18:95 (22 November 2021)
DOI
:10.4103/1735-3327.330876
Background:
This randomized clinical trial (RCT) aimed to compare the 3-year survival rates of high viscosity glass ionomer restorations (HVGIC) using conventional cavity preparation and atraumatic restorative technique (ART) in primary molars.
Materials and Methods:
In this RCT, 139 schoolchildren aged 6–9 years with dentinal caries in primary molars were randomly allocated to groups, i.e. the ART group and the conventional group, utilizing a random number generator. Adequate allocation concealment was done. Intervention was delivered using standard procedure and GC Fuji IX ART HVGIC was used as restorations in both the groups. Analysis was carried in 92 participants, and survival rates in both the groups were compared at 12, 24, and 36-month intervals. IBM SPSS software was utilized to analyze the time taken for the procedure and the Kaplan–Meier estimate was used to assess the survival rates.
P
value of 0.05 was considered statistically significant.
Results:
The ART took longer to complete (16.48 ± 2.02 min) versus conventional rotary instrumentation (13.15 ± 1.32 min). The conventional method was slightly superior as compared to ART; excellent survival rates (i.e. >90%) were achieved in both groups at the end of 12-month follow-up with no significant differences at the end of 24 and 36 months as evident from Kaplan–Meier estimate (
P
= 0.255).
Conclusion:
Three-year follow-up showed that GIC restorations with ART and conventional method carried out using GC Fuji IX ART HVGIC were acceptably successful, substantiating the use of ART for the primary dentition in areas with high caries prevalence and limited access to dental care.
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Original Article:
Drug release kinetics and biological properties of a novel local drug carrier system
Farhad Shafiei, Mehrsima Ghavami-Lahiji, Tahereh Sadat Jafarzadeh Kashi, Farhood Najafi
Dent Res J
2021, 18:94 (22 November 2021)
DOI
:10.4103/1735-3327.330875
Background:
The purpose of this
in vitro
study was to investigate drug release kinetics and cytotoxicity of a novel drug delivery system for treatment of periodontitis.
Materials and Methods:
This
in vitro
study addresses the fabrication of a polycaprolactone/alginic acid-based polymeric film loaded with metronidazole, as a basic drug in the treatment of periodontal diseases. Films were prepared by solvent casting technique. Four formulations with different percentages of drug by weight (3%, 5%, 9%, and 13%) were prepared. Drug release kinetics were investigated using ultraviolet–visible spectroscopy during (one week). Data were analyzed using repeated measures ANOVA. Cytotoxicity of drug-loaded system extracts was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay using L929 cells after 24-h incubation. The results were evaluated according to ISO standard 10993-5 and assessed using ANOVA and Tukey's tests at a significance level of
P
< 0.05.
Results:
All polymeric films showed a burst drug release followed by a gradual release. Drug release data were fitted well with the first-order kinetic model in all drug-containing formulations indicating that drug release is a fraction of remaining drug in the matrix. Drug release is mainly driven by diffusion of medium into the composite matrix. 3%wt metronidazole-containing formulation exhibited the best MTT result.
Conclusion:
The findings of this study supported the synthesis of drug-loaded periodontal films with 3% metronidazole due to better biological properties along with the ability of acceptable drug release to eradicate anaerobic periodontal bacteria.
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Original Article:
A randomized control trial comparing the efficacy of 3Mixtatin and Modified 3Mix-MP paste using lesion sterilization and tissue repair technique to conventional root canal treatment in primary molars of children aged 4–8 years: An
in vivo
study
Seema Thakur, Aman Deep, Parul Singhal, Deepak Chauhan
Dent Res J
2021, 18:93 (22 November 2021)
DOI
:10.4103/1735-3327.330874
Background:
The aim of this study was to evaluate clinical and radiographic success rates of 3Mixtatin and Modified 3Mix-MP paste and compare it with conventional root canal treatment procedure in primary molars requiring pulpectomy.
Materials and Methods:
In this
in vivo
study, 66 primary molars in 52 children aged between 4 and 8 years with primary molars having chronic periapical abscess were treated randomly with 3Mixtatin, Modified 3Mix-MP paste, and Metapex. The subjects were reviewed at 6 and 12 months both clinically and radiographically after pulpal therapy to evaluate and compare the healing process. The data obtained were subjected to statistical analysis at a significance level of 0.05.
Results:
By the end of 12-month follow-up among the three groups, Group I seemed to be performing consistently better as compared to the other two groups when evaluated clinically and radiographically. However, Group III resulted in the greatest number of failures, with success rate being mere 42.9% at the end of follow-up period.
Conclusion:
Radiographic and clinical healing occurred in all the three groups; however, based on our results, Group I seemed to be performing consistently better among the three groups at 12-month follow-up. Hence, it can be inferred that 3Mixtatin used as a localized agent is effective and comparable to both Modified 3Mix-MP paste and conventional pulpectomy procedure involving calcium hydroxide and iodoform paste in primary teeth.
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Original Article:
Factors causing stress in postgraduate dental students during COVID-19 pandemic: A cross-sectional survey
Ruchi Juneja, Neha Sikka, Varun Kumar, Savita Chahal, Mayank Arora, Mitali Middha, Ruchi Singhal
Dent Res J
2021, 18:92 (22 November 2021)
DOI
:10.4103/1735-3327.330873
Background:
COVID-19 pandemic has impacted the academic and clinical training of postgraduate dental students. This study aimed to assess various factors causing psychological stress in them, and the extent of stress perception during pandemic.
Materials and Methods:
In this cross-sectional study, 250 postgraduate students from nine dental colleges of Haryana and National Capital Region, India, responded to an online questionnaire sent electronically to them in September 2020, which included modified dental environment stress (DES) score, Perceived Stress Scale (PSS), and questions regarding COVID-associated stress (CAS). Students already diagnosed with any psychiatric disorder or having a history of any major adverse event during the last 6 months likely to affect their psychological health were excluded from this study. The data obtained were analyzed using Chi-square test, Independent
t
-test, univariate ANOVA with
post hoc
tests, Pearson moment correlation, and multiple hierarchical regression tests.
P
< 0.05 was considered statistically significant.
Results:
DES score was moderate for 48.8% and high for 34.4% of the participants. PSS was moderate for 69.2% and high for 18.8% of the participants. The most stressful factor in dental environment was the pattern of university examination, while the most stressful factor specific to COVID-19 was the fear of family members contracting the infection. PSS score was significantly higher in female participants. DES and CAS scores were significantly higher in students staying in hostels. Multiple hierarchical regression model depicted gender, mean health, and DES score as significant predictors of PSS.
Conclusion:
Postgraduate dental students reported the adverse impact of COVID-19 pandemic upon their training and prospects as a reason for the increase in stress.
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Original Article:
The efficacy of okra fruit extract on the expression of transforming growth factor beta 1 in the tooth socket of diabetic Wistar rats
Muhammad Luthfi, Yuliati , Elvina Hasna Wijayanti, Fathilah Binti Abdul Razak, Wahyuning Ratih Irmalia
Dent Res J
2021, 18:91 (22 November 2021)
DOI
:10.4103/1735-3327.330872
Background:
Patients with diabetes mellitus suffer from an additional macrophage dysfunction in the secretion of growth factor, which later decreases transforming growth factor beta 1 (TGF-β1). This condition disrupts proliferation and angiogenesis. Extract of okra fruit (
Abelmoschus esculentus
) contains flavonoid, an active substance which acts as antioxidant, anti-inflammation, and antidiabetes. The purpose of this study is to analyze the difference in TGF-β1 expression in wound-healing process after tooth extraction of diabetic Wistar rats.
Materials and Methods:
This is a laboratory experimental study using pretest and posttest on 24 Wistar rats which are divided into two groups: control group (treated with streptozotocin induction but without administration of okra fruit extract) and treatment group (treated with streptozotocin induction and oral administration of 250 mg/kg okra fruit extract once a day). Extractions of the rats' mandibular left incisors were performed using a pair of modified forceps and an elevator. The tooth sockets were then irrigated using saline solution. Four rats in each group were sacrificed on day 3 (KO1, PO1), 5 (KO2, PO2), and 7 (KO3, PO3). The socket tissues from the rats were then immunohistochemically analyzed. Data were analyzed at level significance of 0.05.
Results:
The average level of TGF-β1 expression in the treatment groups was higher compared to the control group: PO1 (11.59 ± 0.58), PO2 (15.15 ± 1.07), and PO3 (18.75 ± 2.73) as compared to KO1 (5.32 ± 1.69), KO2 (8.47 ± 0.60), and KO3 (9.28 ± 1.16) with
P
= 0.001.
Conclusion:
The administration of okra fruit extract can increase the level of TGF-β1 in wounds after tooth extraction of diabetic Wistar rats.
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Original Article:
Effect of
Streptococcus mutans
on the flexural strength of resin-based restorative materials
Haleh Valizadeh Haghi, Hadi Peeri-Dogaheh, Sheida Fazlalizadeh, Malek Abazari, Reza Mohammadhosseini
Dent Res J
2021, 18:90 (21 October 2021)
DOI
:10.4103/1735-3327.328758
Background:
There are a limited number of studies about the effects of microbial aging on the mechanical properties of restorative materials. Therefore, this study aimed to evaluate the effect of simulated aging with
Streptococcus mutans
on the flexural strength of different resin-based materials.
Materials and Methods:
This experimental study was performed on the blocks of different types of restorative materials including composite resin, giomer, and a resin-modified glass ionomer (RMGI). Moreover, three types of aging, such as 30-day storage in distilled water,
S. mutans
, and germ-free culture medium, were used in this study. The three-point bending flexural strength of the specimens before and after aging was measured according to the International Organization for Standardization-4049 standard. Data were analyzed by two-way ANOVA and
post hoc
Tukey's tests. A
P
< 0.05 was considered statistically significant.
Results:
Results showed that the 30-day aging with the
S. mutans
significantly reduced the flexural strength of all three types of materials (
P
= 0.00). In all restorative materials, storage in a bacteria-free culture medium acted the same as distilled water, and there was no significant difference between these two solutions in terms of the flexural strength of the material, compared to the before-aging strength (
P
> 0.05). Furthermore, no significant difference was observed between
S. mutans
-based aging and distilled water aging regarding RMGI (
P
= 0.75).
Conclusion:
It can be concluded that aging by
S. mutans
reduced the flexural strength in all three restorative materials.
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Original Article:
Evaluation of microleakage in Class II composite restorations: Bonded-base and bulk-fill techniques
Atiyeh Feiz, Marzieh Sajedi, Niloufar Jafari, Edward J. Swift
Dent Res J
2021, 18:89 (21 October 2021)
DOI
:10.4103/1735-3327.328757
Background:
This study compared microleakage of Class II cavities restored using bonded-base and bulk-fill techniques with different bases.
Materials and Methods:
In this
in vitro
study, in 60 extracted human molars, standardized (4 mm × 2 mm × 8 mm) Class II cavities were prepared, such that the gingival floor was located 1 mm below the CEJ. The teeth were randomly divided into five groups and filled with: (1) Fuji II LC + x-tra fil, (2) Ionoseal + x-tra fil, (3) x-tra base + x-tra fil, (4) Grandio Flow + x-tra fil, and (5) x-tra fil only [control group]; in open-sandwich technique, the base thickness was 1 mm. The bases were coated all gingival floor. Except for the first group, where dentin conditioner was used, the Clearfil SE bond was applied before application of the bases and restorative materials as a bonding agent. After 500 thermocycles between 5°C and 55°C, the specimens were immersed in 0.5% basic fuchsine solution for 24 h. The restored teeth were sectioned, and the dye penetration in gingival floor was observed by a stereomicroscope at ×32. The data were analyzed using Kruskal–Wallis and Mann–Whitney tests in SPSS software. The significance was determined at 0.05 confidence interval.
Results:
The statistical analysis revealed a significant difference in microleakage among the study groups (
P
< 0.001). The Ionoseal group followed by the control group (x-tra fil composite) had the greatest microleakage. Except for the Ionoseal group, all other groups had significantly less microleakage than the control group.
Conclusion:
The use of bonded-base techniques could reduce microleakage, including those in bulk-fill composite restorations.
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Review Article:
Screening of oral squamous cell carcinoma by serum changes: A systematic review and meta-analysis
Forooz Keshani, Saeedeh Khalesi, Alireza Aghaz, Mohammadreza Farhang, Nabiollah Akbari
Dent Res J
2021, 18:88 (21 October 2021)
DOI
:10.4103/1735-3327.328756
Background:
Oral squamous cell carcinoma (OSCC) is the sixth common cancer in the world and 90% of oral malignant tumors. The aim of this study was the investigation of changes in some metabolic elements of OSCC patients' serum.
Materials and Methods:
In this study, international databases such as PubMed, Science Direct, Scopus, Web of Science, and National (Magiran, IranMedex) were searched from 1980 to 2019. To analyze the data, a random-effects model was used to combine the differences in the mean of studies in STATA Software (version 12).
Results:
A total of 724 articles were found with initial searching that 474 duplicate articles, 228 articles were excluded by reviewing the title and abstracts, and 17 articles were excluded from the study due to lack of inclusion criteria. Finally, five articles entered the meta-analysis phase. The mean difference value for zinc concentration of blood serum was 2.01 (95% confidence interval (CI): 0.36–3.66) and for copper was 1.04 (95% CI: 0.01–2.07). In both populations, the heterogeneity was found between studies (
I
2
= 97.4,
P
< 0.001).
Conclusion:
Probably higher serum levels of copper and zinc could be one way to help to do a primary screening of OSCC in suspected patients.
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Original Article:
The effect of silane-containing universal adhesives on the immediate and delayed bond strength of repaired composite restorations
Razieh Hoseinifar, Niloofar Shadman, Farnaz Mirrashidi, Sajad Gholami
Dent Res J
2021, 18:87 (21 October 2021)
DOI
:10.4103/1735-3327.328755
Background:
The repair of composite restorations is considered as a conservative treatment for avoiding the risk of pulp injury, the enlargement of cavity preparation, and excess removal of sound dental structure. The aim of this study was to evaluate the effect of silane-containing adhesives on immediate and delayed shear bond strength (SBS) of repaired composite restorations.
Materials and Methods:
In this
in vitro
study, 132 discs of Z350 composite were fabricated and divided into fresh (10 min water storage) and aged (6-month water storage + 2000 thermal cycling). All composite surfaces were roughened and etched, and each group was divided equally into six subgroups: 1 (Single Bond 2), 2 (Single Bond Universal), 3 (Clearfil Universal Bond), 4 (silane + Single Bond 2), 5 (silane + Single Bond Universal), and 6 (silane + Clearfil Universal Bond). The specimens were restored with the same composite, thermocycled, and tested for SBS in a universal testing machine. Data were analyzed using one- and two-way ANOVA,
t
-test, and
post hoc
Tukey's tests.
P
< 0.05 was set as the level of significant.
Results:
The highest and lowest SBS (in both fresh and aged groups) were related to Single Bond 2 with silane and Clearfil Universal Bond with silane, respectively. The delayed SBS of Single Bond 2 was significantly higher than universal adhesives (in both with and without silane application) (
P
< 0.05). Silane had no significant effect on the repair bond strength of Single Bond 2 and Single Bond Universal (
P
> 0.05), while silane application significantly decreased the delayed SBS of Clearfil Universal Bond.
Conclusion:
The SBS of Single Bond 2 was significantly better than two other universal adhesives. The SBS of Single Bond Universal was not affected by silane application, while silane had a negative effect on delayed SBS of Clearfil Universal Bond.
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Original Article:
Regeneration of dentin-pulp complex by using dental pulp stem cells in dog
Fatemeh Dehghani Nazhvani, Setareh Kazempour, Seyed-Mojtaba Hosseini, Ali Dehghani Nazhvani, Pardis Haddadi
Dent Res J
2021, 18:86 (21 October 2021)
DOI
:10.4103/1735-3327.328754
Introduction:
Although missing tooth is not life-threatening, it affects the quality of daily life. Stem cells have emerged as an important player in the generation and maintenance of many tissues. The role of scaffolds has changed from a passive carrier to a bioactive matrix, which can be used to induce cellular behavior. The aim of this study was to determine the possibility of regeneration of dentin-pulp complex with dental pulp stem cells (DPSCs) in an animal model.
Materials and Methods:
In this animal study after extraction of DPSCs and cultivation, 10 types of scaffolds were made by using platelet-rich plasma (PRP), cancellous bone, and collagen pad. They were inserted in different parts of the dog's mouth. After the 4
th
month, the area was operated, and the scaffolds were removed.
Results:
Microscopic examination revealed no sign of cell differentiation and formation of new structures in those models which used collagen scaffolds. However, the dentin-pulp complex emerged in models that the combination of bone scaffolds and PRP or stem cells was used.
Conclusion:
Using bone scaffolds in combination with PRP or DPSCs to regenerate dentin-pulp complex in dog helped odontoblastic and pulpal differentiation as well as the formation of predentin and tubular dentin.
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Original Article:
Comparison of full rotation and reciprocating movements in regaining apical patency during endodontic retreatment
Hadi Nouri, Kamal Amini, Maryam Zare Jahromi
Dent Res J
2021, 18:85 (21 October 2021)
DOI
:10.4103/1735-3327.328753
Background:
This study aimed to compare the continuous rotation and reciprocating movements of rotary files in achieving apical patency in root canal retreatment.
Materials and Methods:
This
in vitro
, experimental study evaluated 64 extracted mandibular molars. The teeth were prepared up to F3 with ProTaper Universal and obturated using lateral compaction technique. The teeth were divided into four groups (
n
= 16) based on the mesiobuccal canal curvature (Schneider's method) and type of rotational movement. Groups 1 and 2 included straight canal teeth retreated with WaveOne Gold and ProTaper Universal Retreatment system, respectively. Groups 3 and 4 included teeth with moderately curved root canals retreated with WaveOne Gold and ProTaper Universal, respectively. Apical patency was ensured by observing the tip of a hand K-file at the apical foramen. Data were analyzed using the Fisher's exact test.
P
< 0.05 was considered statistically significant.
Results:
Apical patency was successfully achieved in all 16 teeth in group 1 (100%) and 15 teeth in group 2 (93.75%). No significant difference was noted between continuous rotation and reciprocating movements in straight canals (
P
= 1.00). Apical patency was successfully achieved in 15 teeth in group 3 (93.75%) and 12 teeth in group 4 (75%). No significant difference was noted between continuous rotation and reciprocating movements in moderately curved canals either (
P
= 0.333).
Conclusion:
Both continuous rotation and reciprocating movements are equally effective in achieving apical patency in straight or moderately curved mesiobuccal canal of mandibular molars.
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Original Article:
In vitro
comparison of the accuracy (precision and trueness) of eight dental scanners for dental bridge scanning
Fariborz Vafaee, Mahsa Mohajeri, Naser Mohammad Gholi Mezerji, Marouf Ebrahim Zadeh
Dent Res J
2021, 18:84 (21 October 2021)
DOI
:10.4103/1735-3327.328752
Background:
Dental scanners play a critical role in computer-aided design/computer-aided manufacturing technology. This study aimed to compare the accuracy (precision and trueness) of eight dental scanners for dental bridge scanning.
Materials and Methods:
In this
in-vitro
experimental study, a typodont model with a missing maxillary right first molar was prepared for a 3-unit fixed partial denture. Each scanner (Sirona inEos inLab, Sirona X5, Dentium, Imes icore 350I I3D, Amann Girrbach map 100, 3Shape D100, 3Shape E3) performed seven scans of the typodont, and the data were analyzed using 3D-Tool software. The abutment length, abutment width, arch length, and interdental distance were measured. To assess the accuracy of each scanner, trueness was evaluated by superimposing the scanned data on true values obtained by the 3shape Triosscanner as the reference. Precision was evaluated by superimposing a pair of data sets obtained from the same scanner. Precision and trueness of the scanners were compared using the one-way ANOVA followed by the
post-hoc
Tukey's HSD test and one-sample
t
-test (
P
<0.05 was considerer significant).
Results:
The precision of scanners ranged from 14 μm (3Shape Trios) to 45 μm (Imes icore 350i), whereas the trueness ranged from 38 μm (3Shape d700) to 71 μm (Sirona X5).
Conclusion:
The reported trueness values for 3Shape Trios, Sirona inEos inLab, Sirona x5, Dentium, Imes icore350i, Amann Girrbach, 3Shape d700, and 3Shape e3 were 63, 45, 71, 67, 70, 53, 38, and 42 μm, respectively, whereas the precision values were 14, 29, 44, 34, 45, 44, 30 and 28 μm, respectively.
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Original Article:
Knowledge, attitude, and expertise of nurses in intensive care unit regarding oral and dental care in hospitalized patients.
Abbas Haghighat, Iman Mohammadi, Bahareh Tahani, Farshad Teimoori
Dent Res J
2021, 18:83 (21 October 2021)
DOI
:10.4103/1735-3327.328751
Background:
The purpose of this study was conducted to evaluate the knowledge, attitude, and practice of intensive care unit (ICU) nurses about oral and dental care in hospitalized patients.
Materials and Methods:
In this descriptive-analytic study, the statistical population included 214 nurses working in the ICU of the affiliated hospitals of Isfahan University in 1394. The level of knowledge, attitude, and practice of ICU nurses was assessed using questionnaires whose justifiability and stability were verified at the beginning of the study with a pilot study. Data were entered into SPSS software and tested by
t
-test, Spearman, one-way variance, and least significant difference test. The significance level was < 0.05.
Results:
The data of this study showed that the score of knowledge and performance in male nurses was significantly different from female nurses. There was a significant relationship between nurse's education and their knowledge score (
P
< 0.001). Furthermore, the performance score of nurses working in different parts was different too (
P
< 0.001).
Conclusion:
The findings showed that the knowledge and performance of female nurses about oral care were higher than men, but the attitude of the two sexes is almost the same. Nurses with lower educational degree had less knowledge, but their attitude and performance did not differ. The performance score of nurses working in ICU was different, but they had similar knowledge and attitudes.
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Original Article:
Evaluation and comparison number of gingival fibroblast and osteosarcoma cell (MG-63 cell line) adhesive to mocugraft, alloderm, and collagen membrane with or without advanced platelet-rich fibrin
Bentol Hoda Reisie, Shirin Zahra Farhad, Shirin Amini Sadeh
Dent Res J
2021, 18:82 (21 October 2021)
DOI
:10.4103/1735-3327.328750
Background:
The tissue engineering has recently shown a significant progress in the fields of membranes and biosynthetic materials. Advanced platelet-rich fibrin (A-PRF) contains functional molecules that have newly shown great interest in regenerative therapies. The purpose of this study was to evaluate the effect of A-PRF on the adhesion of gingival fibroblast cells and osteosarcoma cells to different membranes.
Materials and Methods:
In this experimental
in vitro
study, three collagen, alloderm, and mucograft membranes were studied, which were cut into four 5 mm × 5 mm pieces and placed in the bottom of a 24-well culture medium. One milliliter of A-PRF was added to two wells from each group and the other two wells remained without A-PRF. The gingival fibroblasts and osteosarcoma cells were individually added to each well. The cell adhesion was studied using an electron microscope after 24 h. The data were analyzed by independent
t
-test, one-way analysis of variance, and least significant difference test.
Results:
In the presence of A-PRF, there was a significant higher osteoblast adhesion to collagen membrane compared to alloderm and mucograft membranes (
P
< 0.001). In the absence of A-PRF, adhesion of osteoblasts to collagen membrane was significantly higher than alloderm and mucograft (
P
= 0.019). Moreover, in the presence of A-PRF, fibroblast adhesion to collagen membrane was significantly higher than alloderm and mucograft membranes (
P
< 0.001). Furthermore, in the absence of A-PRF, no significant difference was found among the study groups (
P
= 0.830).
Conclusion:
A-PRF was effective on fibroblast adhesion to the collagen membrane, which is similar to its absence. A-PRF was also found to be very effective on the adhesion of fibroblast cells to the collagen membrane, and in its absence, even less adhesion was observed compared to the other membranes. The presence or absence of A-PRF showed no significant differences in both cells' adhesion for alloderm and mucograft membranes.
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Original Article:
Effects of a ceramic active self-ligating bracket on retraction/tipping/rotation of canine, premolar mesialization, and transverse arch dimensions: A preliminary single-blind split-mouth randomized clinical trial
Mehrnaz Moradinejad, Nasim Ghorani, Majid Heidarpour, Meysam Noori, Vahid Rakhshan
Dent Res J
2021, 18:81 (21 October 2021)
DOI
:10.4103/1735-3327.328749
Background:
There is no clinical study on ceramic self-ligating brackets (SLBs). Therefore, this preliminary study was conducted for the first time to address its effects.
Materials and Methods:
This split-mouth randomized trial was performed on 32 quadrants in 16 orthodontic patients needing extraction of maxillary premolars and distalization of canines. In each blinded patient, right/left sides were randomized into control (ceramic bracket) and experimental (ceramic SLB) groups. Dental stone models were taken before canine retraction and 3 months into retraction. Models were digitized as three-dimensional models. Changes were measured on superimposed models. Groups were compared using Wilcoxon signed-rank test (α = 0.05, β = 0.1).
Results:
Both bracket types caused significant changes after 3 months in terms of all assessed clinical outcomes (
P
≤ 0.002). Compared to conventional ceramic brackets (control), ceramic SLBs reduced retraction rate
(P
= 0.001), canine rotation
(P
= 0.001), canine tipping
(P
= 0.002), and arch expansion at the canine site
(P
= 0.003). However, the extents of anchorage loss (
P
= 0.796) and arch constriction in the premolar area (
P
= 0.605) were not statistically different between the bracket types.
Conclusion:
Compared to conventional metal-lined ceramic brackets, active ceramic SLB can increase the duration of canine distalization, while reducing canine rotation and tipping (inducing more bodily movements). The loss of anchorage with ceramic SLB was similar to that of conventional ceramic bracket after 3 months of treatment (considering the lower rate of SLB canine retraction during that time). Both brackets similarly constricted the arch at the premolar site. In the canine area, they expanded the arch, with the SLB causing smaller extents of expansion.
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Case Report:
Basaloid squamous cell carcinoma of gingiva: A rare case report
Sunitha Jagaluru Doddanna, Meghanand T Nayak, Aparna K Sanath, Mohammad Zanul Abedeen
Dent Res J
2021, 18:80 (25 September 2021)
DOI
:10.4103/1735-3327.326653
Basaloid squamous cell carcinoma (BSCC) is an exceptional, aggressive variant of squamous cell carcinoma (SCC) because of its unique histological feature and an ominous clinical behavior. Recently, it has been recognized as a high-grade SCC. The most preferential site of occurrence is the upper aerodigestive tract. Because of its aggressive behavior and tendency to metastasize, BSCCs are considered to have poor prognosis. We present a BSCC case in a 60-year-old male, which was clinically diagnosed as an aggressive inflammatory lesion with a differential diagnosis of granulomatous lesion, involving the mandibular anterior gingiva. Till now, only 17 cases of BSCC with gingival involvement have been reported in the literature. Here, we present one additional case of BSCC involving gingiva.
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Original Article:
Association of adipocyte fatty acid-binding protein and tumor necrosis factor alpha with periodontal health and disease: A cross-sectional investigation
Soubiya , Hemalata Madaiah, Fouzia Tarannum, Mohamed Faizuddin
Dent Res J
2021, 18:79 (25 September 2021)
DOI
:10.4103/1735-3327.326652
Background:
Adipocyte fatty acid binding protein (A-FABP) is a novel biomarker of inflammation for various chronic systemic diseases. Since periodontitis is a chronic inflammatory disease, this study explores the association of A-FABP with periodontal disease parameters and tumor necrosis factor-alpha (TNF-α) levels in gingival crevicular fluid in periodontal health and disease.
Materials and Methods:
This original research article describes a cross-sectional study conducted at the Department of Periodontics, M. R. Ambedkar Dental College and Hospital, Bangalore, India. This cross-sectional investigation was conducted on sixty subjects which were divided into three groups of twenty subjects each – healthy, gingivitis, and chronic periodontitis. Clinical parameters – plaque index, bleeding index, probing depth, and clinical attachment loss were recorded. Gingival crevicular fluid samples were analyzed for A-FABP and TNF-α levels using ELISA. One-way analysis of variance was used to find the significance of study parameters on a continuous scale between three groups. Pearson's correlation has been used to find the relationship between Gingival crevicular fluid concentration of markers and periodontal parameters. Multiple linear regression analysis was applied to the study. The statistical significance was considered at
P
< 0.05.
Results:
Mean concentration of A-FABP (6.43 ± 2.51) and TNF-α (3454.82 ± 1566.44) was highest in the periodontitis group, and the difference among the groups was statistically significant (
P
< 0.05). A positive correlation was observed between clinical attachment loss and the two markers among all groups. The correlation between A-FABP and TNF-α in periodontitis groups was positive and statistically significant (
P
< 0.05). Multiple linear regression model was statistically significant (
P
< 0.05) indicating that there is a significant relationship between the set of predictors and the clinical attachment loss.
Conclusion:
A-FABP and TNF-α levels in GCF were significantly elevated in the presence of inflammation. A-FABP has a probable stimulatory effect on TNF-α; however, its role needs to be explored. A-FABP could serve as a novel inflammatory biomarker of periodontitis and the scope of using A-FABP inhibition as a treatment modality could be investigated with interventional studies.
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Case Report:
Retreatment of an orthognathic camouflaged case by total lower arch mesialization using temporary anchorage device
Siamak Hemmatpour, Golnaz Nahvi, Mehdi Oonchi
Dent Res J
2021, 18:78 (25 September 2021)
DOI
:10.4103/1735-3327.326651
Mandibular tooth extractions for camouflage treatments in borderline Class III patients may end up with unpleasing esthetic results which may lead the patient seeking further complicated retreatments. In this case report, we presented a patient's retreatment with a combined orthodontic and orthognathic surgery treatment plan. The treatment strategy involved an innovative temporary anchorage device (TAD)-based method for total mandibular arch mesialization instead of space reopening for first lower premolar replacement with implants. In the current case report, orthognathic management of a Class III adult female is discussed. The patient had a history of an esthetically unsuccessful previous camouflaged orthodontic treatment with two lower premolar extractions. She was prepared for orthognathic surgery using an innovative custom-made sliding jig based on miniscrews for total mandibular arch mesialization with the aim of dental arch decompensation. After gaining the optimal dental arch discrepancy, the patient had orthognathic surgery (maxillary advancement and mandibular setback and advancement genioplasty). Total mandibular dental arch mesialization with the aim of dental arch discrepancy preparation was successfully conducted using TADs. By means of the innovative miniscrew-anchored sliding jig, the patient's satisfactory esthetic and occlusal results were obtained in 22 months. With innovative application of the miniscrews, more practical and reasonable alternatives are available to replace conventional and complicated treatment plans such as space reopening for implants in patients with unsuccessful previous orthodontic therapies.
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Case Report:
Successful management of a tooth with canal obstruction using “cold ceramic”
Jalil Modaresi, Zahra Almodaresi, Rahele Mousavi, Alireza Mirzaeeian, Said Abbas Sadat Hosseini
Dent Res J
2021, 18:77 (25 September 2021)
DOI
:10.4103/1735-3327.326650
One of the causes of treatment failure is calcification in the canal pathway, which prevents complete access to proper working length, and one of the most difficult and challenging cases of root canal treatment has been reported. In some cases, the dentist will not be able to open the calcified canal. Therefore, the prognosis of root canal treatment in these cases has been reported poor, and the dentist will offer a plan for possible treatments, including tooth extraction or apical surgery. The presented case is one example of those cases with a periapical lesion, which as a temporary treatment, the pulp chamber was filled with cold ceramic. This material, which is an MTA-like (Mineral Trioxide Aggregate) bioceramic with proper sealing ability, has been introduced as a root-end filling material. After 6 years of which the patient returned, he did not express any pain or discomfort during these 6 years. Furthermore, in radiograph that was taken, periapical lesion relative healing and bone regeneration were amazingly seen.
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Original Article:
Assessing quality of obturation and instrumentation time using Kedo-SG blue, Kedo-SH, and reciprocating hand K-files in primary mandibular molars: A double-blinded randomized controlled trial
S Sruthi, Ganesh Jeevanandan, Lavanya Govindaraju, Emg Subramanian
Dent Res J
2021, 18:76 (25 September 2021)
DOI
:10.4103/1735-3327.326649
Background:
Pulpectomy is the treatment of choice for severely infected primary molars. The aim of the study is to evaluate the instrumentation time and obturation quality using Kedo SG blue, Kedo SH, and reciprocating hand K files in primary mandibular molars. To evaluate the instrumentation time and obturation quality using Kedo-SG blue, Kedo-SH, and reciprocating hand K-files in primary mandibular molars.
Materials and Methods:
A double-blinded randomized controlled trial was conducted on 45 mandibular molars and were randomly assigned to three experimental groups (
n
= 15). Group I was instrumented using Kedo-SG blue pediatric rotary files, Group II with Kedo-SH pediatric hand files, and Group III with reciprocating hand K-files. Standardized digital radiographs were taken before and after root canal instrumentation. Root canal preparation time was also recorded. Statistical analysis of the obtained data was done using SPSS software version 17.0. An intergroup comparison of the instrumentation time and the quality of obturation were done using ANOVA and Chi-square test with the level of significance set at 5%.
P
< 0.05 was considered statistically significant.
Results:
No significant differences were noted with regard to the quality of obturation between the three groups (
P
< 0.14). However, a statistically significant difference was noted in the instrumentation time between the three groups (
P
= 0.000). Kedo-SG blue rotary file had significantly lesser instrumentation time when compared to that of Kedo-SH hand files and reciprocating hand K-files.
Conclusion:
On comparative evaluation
,
Kedo-SG blue pediatric rotary file showed a marked reduction in instrumentation time, followed by Kedo-SH pediatric hand files and reciprocating hand K-files.
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Original Article:
An experimental study on the effect of four pediatric drug types on color stability in different tooth-colored restorative materials
Taraneh Faghihi, Zohreh Heidarzadeh, Karim Jafari, Ilnaz Farhoudi, Somayeh Hekmatfar
Dent Res J
2021, 18:75 (25 September 2021)
DOI
:10.4103/1735-3327.326648
Background:
One of the drawbacks of tooth-colored dental restorations is their discoloration over time. The present study aimed to determine the effect of four categories of pediatric medications, including analgesics, antibiotics, anticonvulsants, and multivitamins, on two types of tooth-colored dental materials, namely, composite resins and glass ionomer cements.
Materials and Methods:
In this
in vitro
study, a total of 40 specimens with disc shapes (with a diameter of 5 mm and thickness of 2 mm) were prepared from each material and immersed in eight different drugs for 2 min three times a day for 1 week. The values of the baseline color were calculated based on the CIE (International Commission on Illumination) L*a*b* system. After 7 days, ΔE values were calculated. Two-way analysis of variance was employed for statistical analysis. Statistical significance was defined at 0.05.
Results:
Statistical analysis showed that there were significant differences between ΔE and different restorative materials as well as ΔE and drug types (
P
< 0.001).
Conclusion:
According to the results of this
in vitro
study, all the four types of drugs caused the discoloration in all the restorative materials, and the color change values were affected by the type of used drug and restorative material.
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Original Article:
Effects of probiotic and fluoride mouthrinses on
Streptococcus mutans
in dental plaque around orthodontic brackets: A preliminary explorative randomized placebo-controlled clinical trial
Sepideh Dadgar, Amin Heydarian, Farhad Sobouti, Hamidreza Goli, Vahid Rakhshan, Mohadeseh Heidari
Dent Res J
2021, 18:74 (25 September 2021)
DOI
:10.4103/1735-3327.326647
Background:
Although it is shown that probiotic agents might reduce
Streptococcus mutans
, no study has evaluated this effect in the form of probiotic mouthrinse. The purpose of this study was to compare the effect of probiotic experimental mouthwash
Lactobacillus plantarum
versus sodium fluoride and placebo mouthwashes on the number of
S. mutans
present in dental plaque around orthodontic brackets in fixed orthodontic patients.
Materials and Methods:
This study was a randomized clinical trial. The total of 38 patients participate consisting of 12 patients in the fluoride group, 13 in the probiotic, and 12 in the placebo group. They were given mouthwashes to use twice a day for 2 weeks. Plaque sampling was performed using the 4-pass technique in all three groups in two stages: before the intervention and after 2 weeks of using the mouthwash. The number of bacteria present in the dental plaque was then reported based on the number of colonies grown on agar medium. Data were analyzed using Kruskal–Wallis and Wilcoxon tests (α = 0.05).
Results:
Gender distribution, mean age, and protocol adherence were not significantly different among all three groups. After the intervention, the number of
S. mutans
present in the dental plaque followed an increasing manner in the placebo (
P
= 0.005) and probiotic (
P
= 0.158) groups and decreased in fluoride group (
P
= 0.025).
Conclusion:
The
L. plantarum
probiotic mouthwash was ineffective in reduction of
S. mutans
in dental plaque. However, fluoride mouthwash is considerably effective against
S. mutans
and thus recommended.
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Original Article:
Effect of zinc oxide nanoparticles on physical and antimicrobial properties of resin-modified glass ionomer cement
Zahra Malekhoseini, Mohammad Bagher Rezvani, Mohammad Niakan, Mohammad Atai, Mahshid Mohammadi Bassir, Hamid Safar Alizade, Saba Siabani
Dent Res J
2021, 18:73 (25 September 2021)
DOI
:10.4103/1735-3327.326646
Background:
To improve the limitations, many modifications in the resin-modified glass ionomer (RMGI) composition have been proposed. In this study, we evaluated the effect of different concentrations of zinc oxide (ZnO) nanoparticles incorporated into RMGI cement on its physical and antimicrobial properties.
Materials and Methods:
In this
in vitro
study, ZnO nanoparticles with 0–4 wt.% concentrations were incorporated into RMGI. The following tests were carried out: (a) Antibacterial activity against
Streptococcus mutans
tested by disc diffusion method, (b) mechanical behavior assessment by measuring flexural strength (FS) and flexural modulus (FM), (c) micro-shear bond strength (μ-SBS), and (d) fluoride and zinc release. Data were analyzed using the statistical tests of ANOVA, t-test, and Tukey's HSD
post ho
c in SPSS V22. The level of significancy was 0.05.
Results:
In the disc diffusion method, specimens with 2 wt.% ZnO nanoparticles showed the highest antimicrobial efficacy (
P
< 0.05). After 1 month of water storage, no significant difference was observed in FS and FM of the samples (
P
> 0.05). In 2 wt.% ZnO nanoparticles group, μSBS increased in the first 7 days but decreased by 17% after one month, which showed a significant difference with that of the control group. The fluoride release did no change in the ZnO nanoparticle-containing group compared with the control group at all time intervals.
Conclusion:
Incorporation of 2 wt.% ZnO nanoparticles into the RMGI cement adds antimicrobial activity to the cement without sacrificing FS and fluoride release properties, while decreased μSBS.
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Review Article:
Effect of total-etch and self-etch adhesives on the bond strength of composite to glass-ionomer cement/resin-modified glass-ionomer cement in the sandwich technique – A systematic review
Amarjot Kaur D. S. Manihani, Sanjyot Mulay, Lotika Beri, Rajesh Shetty, Shreya Gulati, Raj Dalsania
Dent Res J
2021, 18:72 (25 September 2021)
DOI
:10.4103/1735-3327.326645
The success of the sandwich technique depends on the bond strength of composite to glass-ionomer cement (GIC)/resin-modified glass-ionomer cement (RMGIC). Currently used adhesives employ the total-etch and the self-etch techniques. The total-etch system is technique sensitive, whereas the self-etch system is popular for its ease of use. The aim of this systematic review is to compare the effect of total-etch and self-etch adhesives (SEAs) on the bond strength of composite to GIC/(RMGIC) in the sandwich technique. A literature search was conducted using electronic databases (PubMed, Web of Science, Ebscohost, and Scopus) limiting the year of publications from January 1, 2000, to September 30, 2018, to identify the relevant studies. All the cross-references of the selected studies were also screened.
In vitro
studies on extracted human teeth were selected. A total of 10 articles were included in this review. A conclusion was drawn that SEAs when used in the sandwich technique resulted in greater bond strength in comparison to total-etch adhesives. Moreover, increased bond strength was achieved when the primer was employed on unset GIC as compared to set GIC. Furthermore, the application of SEAs over uncured RMGIC (co-curing technique) resulted in better bond strengths as compared to their application over cured RMGIC.
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Original Article:
Evaluation effect of color in dental office and dentist's uniform while using two different distraction techniques on injection anxiety of 6–9 years' old children referring to Hamedan Dental School: Randomized clinical trial
Rezvan Rafatjou, Bahar Ahmadi, Maryam Farhadian, Niloofar Entezari Moghadam
Dent Res J
2021, 18:71 (25 September 2021)
DOI
:10.4103/1735-3327.326644
Background:
The management of children's anxiety in dental offices is still a challenge for the treatment team. The most important factors which affect children's dental anxiety are the clinician-related factors and the physical factors of the dental operatory. Color is an environmental factor whose relationship with the perceptive and emotional factors of children has been reported. On the other hand, distraction is essential factors in a treatment environment that can be used to manage children's anxiety.
Materials and Methods:
In this randomized clinical trial study, from 132 6 to 9 years of age children, 52.3% were male, and 47.7% were female, with a mean age of 7.45 ± 1.1 years. The children's anxiety levels were determined using the Venham Picture Test and pulse oximetry before and after the administration of local anesthesia. The children were randomly assigned to two groups (
n
= 66): Group 1, music, and Group 2, animated cartoons. The data were analyzed with SPSS 25, using descriptive statistics and the statistical tests consisting of one-way analysis of variance (ANOVA),
t
-test, and repeated-measures ANOVA, followed by Tukey honestly significant difference tests. Statistical significance was set at
P
< 0.05.
Results:
The anxiety levels were the lowest in the blue and pink environments and the highest in the black and red. Anxiety levels between different colors in terms of physiologic markers and the questionnaire in each distraction technique at different intervals was significantly different.
Conclusion:
The use of blue, pink, green, and yellow for dentists' attire, and the interior design of the dental operatory decreased the child patients' anxiety.
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Original Article:
Evaluation of apoptotic effect of crocin, cisplatin, and their combination in human oral squamous cell carcinoma cell line HN5
Fatima Bijani, Ebrahim Zabihi, Ali Bijani, Hamid Reza Nouri, Shima Nafarzadeh, Maryam Seyedmajidi
Dent Res J
2021, 18:70 (18 August 2021)
DOI
:10.4103/1735-3327.324029
Background:
Squamous cell carcinoma (SCC) is the most common oral malignancy with high rate of mortality. Cisplatin, as the most effective chemotherapy drug, has side effects. Considering the studies on the use of crocin in saffron in the treatment of various malignancies, this study aimed at investigating the effects of crocin and cisplatin and their combination on SCC and fibroblast cell lines.
Materials and Methods:
In this interventional study, HN5 and fibroblast cell lines were treated with different concentrations of crocin (12.5–50 μg/mL) and cisplatin (2, 4, 8, 16, and 32 μg/mL), and the cells were counted after 24, 48, and 72 h by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Data were analyzed with SPSS Version 17, and
P
< 0.05 was considered the level of significance. In the final stage, flow cytometry after 24 h in terms of the pattern of cell death was done.
Results:
Both drugs had a toxic effect on malignant cells. One point was the high toxic effect of 8 μg/mL cisplatin not only on cancer cells (
P
< 0.001) but also on fibroblasts. However, combination with 12.5 μg/mL of crocin had the same effect on HN5 cell line, despite the less toxic effect in fibroblasts in comparison with cisplatin alone (
P
= 0.012). Apoptosis was the pattern of cell death showed by flow cytometry.
Conclusion:
Crocin in high concentrations can have not only significant toxicity in cancer cells but also side effects in healthy tissue. It seems that lower doses of crocin, in combination with cisplatin, besides having anticancer effect, can reduce the toxicity of cisplatin in healthy tissue.
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Original Article:
Lingual retainer materials: Comparative evaluation of wear resistance of flowable nanocomposites and universal composite: An
in vitro
study
Mohsen Nosouhian, Mohamad Monirifard, Fateme Gharibpour, Saeed Sadeghian
Dent Res J
2021, 18:69 (18 August 2021)
DOI
:10.4103/1735-3327.324028
Background:
A bonded fixed retainer is used to stabilize the alignment of the teeth. Different composites have been introduced for this purpose. This study aimed to investigate the wear resistance of flowable nanocomposite in comparison with microhybrid composite in an
in vitro
situation.
Materials and Methods:
In this
in vitro
study, 46 disk-shaped specimens were divided into two groups: Filtek Ultimate flowable composite and Z250 microhybrid composite. The samples were prepared in 8 mm diameter and 3 mm thickness in an aluminum mold and light cured. They were polished with 600 grit sandpaper to achieve a smooth surface. Two-body wear test was accomplished by the pin-on-disk device (under 15 N, 20 rpm for 1 h). Analyzing the weight and thickness of specimens before and after the assay demonstrates the wear resistance. Data were analyzed using the
t
-test.
P
≤ 0.05 was considered statistically significant.
Results:
The Filtek Ultimate flowable composite shows no significant difference compared to Z250 microhybrid composite in thickness (
P
= 0.701) and weight (
P
= 0.939) of specimens.
Conclusion:
Due to wear resistance of both materials, flowable composite can be recommended as an alternative material for bonded fixed retainers.
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Original Article:
Comparative clinical evaluation of modified and conventional Grayson's presurgical nasoalveolar molding technique in infants with complete unilateral cleft lip and palate
Seema Thakur, C Jishad, Parul Singhal, Deepak Chauhan
Dent Res J
2021, 18:68 (18 August 2021)
DOI
:10.4103/1735-3327.324027
Background:
Presurgical nasoalveolar molding (PNAM) was introduced by Grayson
et al
., in 1993 to presurgically mold the alveolus, lip, and nose in infants with cleft lip and palate (CLP). The aim of this comparative clinical trial was to evaluate the efficacy and efficiency of Modified and Conventional Grayson's PNAM in patients concerning morphological and anatomical changes in maxillary alveolus, nasal symmetry, number of visits, and duration of treatment.
Materials and Methods:
In this comparative clinical trial study, 16 infants with unilateral complete CLP were equally divided into two groups: Group I (modified PNAM technique using titanium molybdenum alloy [TMA] wire nasal stent) and Group II (conventional PNAM technique using stainless steel wire nasal stent). Patient photographic evaluation of nasal symmetry and maxillary study model CAD-CAM analysis, pre- and post-operatively in both groups, were compared using a paired
t
-test between the groups using the Chi-square test with
P
< 0.05 as statistically significant.
Results:
In both groups, on evaluating nasal measurements, statistically significant (
P
< 0.05) decrease in nasal width and increase in columella deviation angle, a decrease of nostril length, and an increase of columella length in Group I were observed. On maxillary study model evaluation, a statistically significant (
P
< 0.05) decrease in width of the alveolar cleft was noticed in both groups and lateral deviation of the incisal point in Group I and width of the palatal cleft in Group II was noticed.
Conclusion:
This study showed a morphological improvement in nasal symmetry and maxillary alveolar morphology in complete unilateral CLP patients, treated with both Modified and Conventional PNAM techniques, with the Modified PNAM technique being more efficient for treatment duration and the number of adjustments as there are less number of visits.
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Original Article:
Oral manifestations among COVID-19: An observational study of 713 patients
Tulsi Subramaniam, Monali R Nikalje, Sameer Jadhav
Dent Res J
2021, 18:67 (18 August 2021)
DOI
:10.4103/1735-3327.324026
Background:
COVID-19 outbreak in 2019 took the entire world by a storm with the medical fraternity struggling to understand and comprehend its complex nature. A number of patients who are COVID positive have reported oral lesions. However, there is still a lingering question, whether these lesions are because of coronavirus infection or they are secondary to the patient's systemic condition. This article aims to report the oral findings of an observational study of 713 patients diagnosed with COVID-19.
Materials and Methods:
A singlssswe-institution, short-term observational study was conducted on patients admitted to Symbiosis University Hospital and Research Centre, Lavale, Pune who were positive to coronavirus, who presented varied oral findings such as herpes simplex, candidiasis, geographic tongue, and aphthous ulcer.
Results:
A total of 713 patients, 416 males and 297 females, who were positive to coronavirus, were screened from April 2020 to June 30, 2020, for oral ulcers. In this group, nine patients reported oral discomfort due to varied forms of oral lesions ranging from herpes simplex ulcers to angular cheilitis (1.26%).
Conclusion:
This study supports the hypothesis that oral manifestations in patients diagnosed with COVID-19 could be secondary lesions resulting from local irritants or from the deterioration of systemic health or could be just coexisting conditions. No specific pattern or characteristic oral lesions were noted in a study of 713 COVID-positive patients in our study to qualify these lesions as oral manifestations of SARS-CoV-2 infection.
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Original Article:
IL29 expression in gingival tissues of chronic periodontitis and aggressive periodontitis patients: An immunohistochemical analysis
Zahra Alizadeh Tabari, Somayeh Hematzadeh, Forooz Keshani
Dent Res J
2021, 18:66 (18 August 2021)
DOI
:10.4103/1735-3327.324025
Background:
Interleukin-29 (IL-29) is one of the cytokines which has immunomodulatory properties and might play a role in the pathogenesis of periodontal diseases. The aim of this study was an immunohistochemical analysis of IL-29 in gingival tissues of chronic and aggressive periodontitis.
Materials and Methods:
In this cross-sectional study based on clinical evaluation and inclusion and exclusion criteria, 20 patients with generalized chronic periodontitis, 13 patients with generalized aggressive periodontitis, and 20 periodontally healthy individuals were enrolled. Gingival tissue samples were obtained during periodontal flap and crown lengthening surgery in periodontal patients and healthy individuals, respectively. Tissue samples were examined to determine the level of IL-29 expression by immunohistochemistry. The data were analyzed using SPSS and paired
t
-test, ANOVA test, and Tukey's test (
P
< 0.05).
Results:
A total of 53 participants (34 females and 19 males) were enrolled in this study. IL-29 expression in the connective tissue of the patient groups was more than the healthy one (
P
< 0.001). In the aggressive periodontitis group, there was a significant increase of IL-29 expression compared to the other two groups, but there was no significant difference between the chronic periodontitis and healthy groups.
Conclusion:
According to the results of this study, IL-29 expression was increased in the gingival tissue of aggressive and chronic periodontitis. IL-29 local expression in aggressive periodontitis is higher than the chronic periodontitis and healthy groups, which could suggest the role of IL-29 in the etiopathogenesis of aggressive periodontitis.
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Review Article:
The effect of complete dentures on edentulous patients' oral health-related quality of life in long-term: A systematic review and meta-analysis
Angela M. C. Martins, Ludmila S. Guimarães, Camila H. Campos, Erika C. Küchler, Daniele M. S. Pereira, Lucianne C. Maia, Leonardo S. Antunes, Lívia A. A. Antunes
Dent Res J
2021, 18:65 (18 August 2021)
DOI
:10.4103/1735-3327.324024
Background:
To evaluate whether the long-term use of complete dentures (CD) into promotes significant changes in the oral health-related quality of life (OHRQoL) in edentulous patients.
Methods:
A systematic review and meta-analysis was conducted. A broad search in Pubmed, Web of Science, Scopus, Cochrane Library, Grey Literature, clinical trials registers and manual search was done. The eligibility criteria were based on population, intervention, comparisons and outcome: (P) edentulous patients, (I) CDs rehabilitation, (C) OHRQoL after CD, (O) change in scores of OHRQoL. Two independent reviewers applied the eligibility criteria, collected qualitative data, performed methodological quality and evaluated the certainty of the evidence (grading of recommendations assessment, development and evaluation). The meta-analysis was analyzed in RevMan 5.4 with 95% confidence intervals (CIs) and
P
< 0.05.
Results:
A total of 2452 records were identified. Twenty-four articles were included in qualitative synthesis. Nineteen studies were qualified as good, 3 as fair and 2 as poor quality. Twelve studies were included in quantitative analysis (meta-analysis). The use of CD did not improved OHRQoL in a period of 3 months through the assessment of the Geriatric Oral Health Assessment Index (GOHAI) instrument (
P
= 0.55; CI; 6.86 [−15.60, 29.31]), and Oral Health Impact Profile-14 (OHIP-14) (
P
= 0.05; CI; −14.91 [−29.87, 0.04]), with very low certainty of evidence. In a long term, 6 months, GOHAI instrument (P < 0.00001; CI; 16.22 [10.70, 21.74]), OHIP 20 (
P
= 0.02; CI; −11.09 [−20.54, −1.64]) and OHIP-EDENT (
P
= 0.0004; CI; −8.59 [−13.32, −3.86]) showed improvement on OHRQoL, with very low and low evidence of certainty, respectively.
Conclusion:
CD has the strong potential to contribute to oral health-related quality of life in long-term.
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Original Article:
Mechanical and antimicrobial property of different surface treated glass ionomer cements under desiccated condition
Hemalatha Hiremath, Chhaya Harinkhere, Pooja Misar, Kshitij Sabley, Trupti Bajpai
Dent Res J
2021, 18:64 (18 August 2021)
DOI
:10.4103/1735-3327.324023
Background:
The purpose of this
in vitro
study was to evaluate the effect of five different surface treatments on the mechanical property and antimicrobial effect of three desiccated glass ionomer cements.
Materials and Methods:
In this
in vitro
experimental study, 300 rectangular blocks of three different restorative materials were fabricated using an aluminum mold, Group I (
n
= 100) Micron bioactive, Group II (
n
= 100) GC Fuji IX GP Extra, and Group III (
n
= 100) bioglass R. These blocks were stored in 100% humidity for 24 h and then placed in air to desiccate for another 24 h. These groups were further divided into two major groups (
n
= 50) for both mechanical (Flexural) and antimicrobial testing. The blocks of mechanical and antimicrobial groups were further divided into five subgroups (
n
= 10) based on the medias used for surface treatment (senquelNaF, MI varnish, chlorhex plus, kedodent mouthwash, and 100% humidity [control]). Flexural strength (FS) was measured using the universal testing machine. Fracture strength of groups was compared using the one-way analysis of variance and Tukey's
post hoc
test with
P
≤ 0.05 considered statistically significant. Antimicrobial effect was carried out by covering the specimens in a suspension of
Streptococcus mutans
followed by incubation for 24 h. The blocks were later washed, vortex mixed, serially diluted, and plated. Ccolony-forming unit/ml was calculated after 3 days of incubation. Data were then analyzed using the Kruskal–Wallis and Mann–Whitney
U
nonparametric test, with
P
≤ 0.05 considered statistically significant.
Results:
Micron bioactive with the surface treatment of MI varnish significantly exhibited highest FS. Surface treatment of desiccated restorative materials with chlorhex plus exhibited no growth of
S. mutans
. GC Fuji IX GP Extra with surface treatment of MI varnish exhibited highest reduction in
S. mutans
growth compared to other experimental group.
Conclusion:
Surface treatment of restorative material with MI varnish improved their mechanical and antimicrobial property while among three restorative materials Micron bioactive showed better mechanical property, whereas GC Fuji IX GP Extra exhibited better antimicrobial property.
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Original Article:
Histological assessment of the local effect of different concentrations of aminoguanidine hydrochloride on bone healing in rats
Ali Reza Farhad, Fahimeh Razavi, Sayed Mohammad Razavi, Masoud Saatchi, Maziar Manshaei
Dent Res J
2021, 18:63 (18 August 2021)
DOI
:10.4103/1735-3327.324022
Background:
Nitric oxide (NO) has several functions in bone healing and affects bone metabolism. Selective inducible NO synthase (iNOS) inhibitors can be used to assess the efficacy of NO for healing of bone defects. This study sought to assess the local effect of different concentrations of aminoguanidine hydrochloride (AG), a selective iNOS inhibitor, on bone healing in rats.
Materials and Methods:
In this animal experimental study, 72 rats were divided into six groups of control, placebo, 5% AG, 10% AG, 15% AG, and 20% AG. A bone defect measuring 5 mm × 5 mm was created in the femur. The defect remained empty in the control group. In the placebo group, neutral gel was placed in the bone defect, and in the remaining four AG groups, different concentrations of AG were applied to the defects. Bone healing was assessed histologically. The healing score in the six groups was analyzed by the Kruskal–Wallis test. A
P
< 0.05 was considered statistically significant.
Results:
The healing score in 20%, 15%, 10%, and 5% AG groups was significantly higher than that in the neutral gel and control groups (
P
< 0.01). Among the four groups of AG, 20% concentration showed better results, but the difference was not significant.
Conclusion:
Four concentrations of AG caused greater bone healing compared to the other two groups. Selective iNOS inhibitors such as AG can be used to promote local bone healing.
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Original Article:
The effect of Vitamin E supplementation on treatment of chronic periodontitis
Parichehr Behfarnia, Mina Dadmehr, Seyedeh Negin Hosseini, Seyed Amir Mirghaderi
Dent Res J
2021, 18:62 (18 August 2021)
DOI
:10.4103/1735-3327.324021
Background:
The purpose of this study was to evaluate the effect of Vitamin E supplements on chronic periodontitis based on the clinical parameters of pocket depth and clinical attachment level and total antioxidant capacity (TAC) of saliva.
Materials and Methods:
In this clinical trial, 16 patients with chronic periodontitis were selected and divided into two groups. The indices of pocket depth and attachment loss for 6 teeth per person were measured with a periodontal probe. A total of 41 teeth in the control group and 42 teeth in the case group were examined. Then, 2 ml nonstimulated saliva was collected from each patient. All patients were treated with scaling and root planing (SRP). The case group consumed 200 IU supplementary Vitamin E daily for up to 2 months. After 2 months, clinical indices were re-measured and 2 ml nonstimulated saliva was collected. The TAC of saliva samples was measured by using Zellbio's TAC Kit. Data were analyzed by the SPSS software and were evaluated in each group between the first session and 2 months later with paired
t
-test. The differences between the two groups were evaluated through the independent
t
-test (α ≤ 0.05).
Results:
Independent
t
-test showed that mean change in TAC (
P
= 0.14) and pocket depth changes (
P
= 0.33) was not significant between two groups 2 months after SRP, but mean attachment loss changes in the case group was significantly less than the control group (
P
= 0.03).
Conclusion:
The results of this study indicate that Vitamin E supplementation with SRP can reduce the inflammatory process of periodontitis and improve periodontal clinical indices and decrease the amount of attachment loss.
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Original Article:
Effects of at-home and in-office bleaching and three composite types (hybrid, microhybrid, and nanofilled) on repair shear bond strength of aged composites: A preliminary study
Faramarz Zakavi, Najmeh Johar, Mina Moalemnia, Vahid Rakhshan
Dent Res J
2021, 18:61 (18 August 2021)
DOI
:10.4103/1735-3327.324020
Background:
Only a few controversial studies have assessed the repair bond strength of a fresh composite to aged composite. Moreover, no studies exist on repair bond strength of fresh composites to bleached composites. Therefore, this preliminary study was conducted to assess repair shear bond strength (SBS) of three composites bonded to nonbleached and at-home and in-office bleached composites.
Materials and Methods:
In this experimental
in vitro
study, 108 disks (36 specimens per composite) of hybrid, microhybrid, and nanofilled composites were divided into three subgroups of three bleaching treatments: no bleaching (control), at-home bleaching, and in-office bleaching. Composite disks were incubated for 4 weeks in artificial saliva (also dipped in tea and coffee for 3 h a day). They were then thermocycled (5000 cycles). Afterward, the control group remained unbleached, while the other groups were bleached according to office and home bleaching methods. They were repaired with the same composite type. Their repair SBS and mode of failure were measured and analyzed using two-way ANOVA, Tukey, one-sample
t
-test, and Chi-square tests (α = 0.05, β = 0.2).
Results:
The mean (standard deviation) SBS values of hybrid, microhybrid, and nanofilled composites were 20.71 ± 5.99, 21.06 ± 6.68, and 9.46 ± 4.32 MPa, respectively. The mean SBS values of the bleaching techniques “home bleaching, office bleaching, and no bleaching (control)” were, respectively, 16.35 ± 7.13, 16.39 ± 8.07, and 18.49 ± 8.35 MPa. There was a significant difference among composites (two-way ANOVA
P
= 0.000) but not among nonbleaching/bleaching methods (
P
= 0.176). Their interaction was significant (
P
= 0.017). The difference between hybrid and microhybrid was not significant. Nevertheless, nanofilled had significantly poorer results compared to both hybrid and microhybrid composites (Tukey
P
= 0.000). Both hybrid and microhybrid were capable of producing satisfactory clinical repair bond strengths (above 20 MPa) regardless of bleaching or lack of it. Nanofilled composite failed to provide proper repair SBS values, even in the control (no-bleaching) group. By moving from Z100 or from Z250 to Z350, modes of failure shifted from mostly cohesive to mostly adhesive (
P
< 0.05).
Conclusion:
Bleaching of an aged composite might not affect the repair bond strength. Hybrid and microhybrid composites can provide clinically acceptable repair bond strengths, regardless of bleaching. Nonetheless, nanofilled composite is inferior to them and cannot provide appropriate repair bond strengths (regardless of bleaching).
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Original Article:
Fracture resistance of endodontically treated premolars restored with bulk-fill composite resins: The effect of fiber reinforcement
Fereshteh Shafiei, Paria Dehghanian, Nasibeh Ghaderi, Maryam Doozandeh
Dent Res J
2021, 18:60 (19 July 2021)
DOI
:10.4103/1735-3327.321871
Background:
Coronal restoration of endodontically treated teeth (ETT) with mesio-occluso-distal (MOD) cavities is of a great importance in long-term success of the treatment. This study evaluated the effect of fiber reinforcement on the fracture resistance (FR) of ETT restored with flowable or paste bulk (PB)-fill composite resin compared to conventional composite (CC) resin.
Materials and Methods:
In this
in vitro
experimental study, eighty maxillary premolars were divided into eight groups (
n
= 10). The first group was left intact (G
1
) and the other groups received MOD cavities along with endodontic treatment. G
2
: Remained unrestored while the other experimental groups were restored with three types of composite resin with or without fiber insertion. G
3
: CC resin, G
4
: PB fill, G
5
: Flowable bulk fill (FB). G
6
: Fiber + CC, G
7
: Fiber + PB, and G
8
: Fiber + FB. FR was tested at crosshead speed of 1 mm/min and recorded in Newton. Data were analyzed using one-way analysis of variance and Tukey's tests at significance level of
P
< 0.05.
Results:
G
1
and G2 revealed the highest and the lowest FR, respectively. The mean FR of the testing groups in Newton was as follows: G
1
= 1204.8
A
, G
2
= 352.1
C
, G
3
= 579.6
BD
, G
4
= 596.7
BD
, G
5
= 624.9
BDE
, G
6
= 858.3
E
, G
7
= 529.6
CB
, and G
8
= 802.5
DE
. Different uppercase letters indicate the significant difference between the groups.
Conclusion:
The effect of fiber insertion on FR depended on the type of composite resin; the highest reinforcing effect was obtained in the CC resin + fiber, followed by bulk-fill flowable + fiber, and flowable bulk (FB)-fill composite resin. The strength of the former was significantly higher than the conventional and PB fill with and without fiber.
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Original Article:
Antibacterial effect of a disinfectant spray for sports mouthguards on Streptococcus
sobrinus
Dent Res J
2021, 18:59 (19 July 2021)
DOI
:10.4103/1735-3327.321870
Background:
Mouthguard (MG) disinfectant sprays are available for maintaining MG hygiene. The effect of these sprays against
Streptococcus sobrinus
is still unknown. The purpose of this study was to evaluate the antibacterial effect of an MG disinfectant spray against
S. sobrinus
using the modified ISO 22196 standard.
Materials and Methods:
In this
in vitro
study, we used the following treatment groups for antibacterial testing: MG spray-1 (left in spray for 30 s), MG spray-2 (60 s), and control (
n
= 4). All analyses were performed at a statistically significant level (
P
= 0.05) using JMP
®
14.
Results:
The log colony-forming units of the MG spray-2 group were significantly lower than those of the other groups. The antibacterial activity of MG spray-2 against
S. sobrinus
was >2.1.
Conclusion:
We confirmed the antibacterial effect of the MG spray against
S. sobrinus
, and it was influenced by the treatment duration, with the optimum effect at a longer duration.
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Original Article:
Bond strength and microleakage of different types of cements in stainless steel crown of primary molar teeth
Somayeh Kameli, Fatemeh Khani, Marjan Bahraminasab, Raheb Ghorbani, Fatemeh Mashhadi Abbas
Dent Res J
2021, 18:58 (19 July 2021)
DOI
:10.4103/1735-3327.321869
Background:
The margin of crown is a significant area for plaque accumulations. Therefore, the ability of the cement to seal the margin is very important. The aim of the present study was to evaluate the bond (retentive) strength, microleakage, and failure mode of four different types of cements in stainless steel crown (SSC) of primary molar teeth.
Materials and Methods:
In this experimental study, eighty extracted primary molar teeth were divided into two groups of forty teeth to test the microleakage and bond strength. The crowns were cemented according to the manufacturer guidelines with four cement types including self-cure glass ionomer, resin-modified glass ionomer, polycarboxylate, and resin cements. Stereomicroscope and universal testing machine were used to measure the microleakage and bond strength, respectively. For calculating the surface area of crowns, three-dimensional scanning was used. Furthermore, the failure mode was examined after the bond strength test. The cements surfaces and the tooth–cement interfaces were evaluated using scanning electron microscopy (SEM). The obtained values were analyzed using SPSS-23 software through Shapiro–Wilk and one-way analysis of variance tests. Means, standard deviations, medians, and interquartile ranges were calculated.
P
< 0.05 was considered as statistically significant in all analyses.
Results:
Significant differences between microleakage (
P
= 0.001) and failure mode (
P
= 0.041) of the four types of cements were obtained. However, the mean bond strengths of the four groups did not differ significantly (
P
= 0.124). The obtained SEM images confirmed the results of bond strength and microleakage.
Conclusion:
Resin cement and resin-modified glass ionomer, respectively, showed superior properties and are recommended for use in SSCs of primary molar teeth.
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Original Article:
Neurogenic induction of human dental pulp derived stem cells by hanging drop technique, basic fibroblast growth factor, and SHH factors
Safa Farhang, Mitra Soleimani, Maryam Ostadsharif, Nazem Ghasemi
Dent Res J
2021, 18:57 (19 July 2021)
DOI
:10.4103/1735-3327.321868
Background:
The progressive destruction of nerve cells in nervous system will induce neurodegenerative diseases. Recently, cell-based therapies have attracted the attention of researchers in the treatment of these abnormal conditions. Thus, the aim of this study was to provide a simple and efficient way to differentiate human dental pulp stem cells into neural cell-like to achieve a homogeneous population of these cells for transplantation in neurodegenerative diseases.
Materials and Methods:
In this basic research, human dental pulp stem cells were isolated and characterized by immunocytochemistry and flow cytometry techniques. In the following, the cells were cultured using hanging drop as three-dimensional (3D) and tissue culture plate as 2D techniques. Subsequently, cultured cells were differentiated into neuron cell-like in the presence of FGF and Sonic hedgehog (SHH) factors. Finally, the percentage of cells expressing Neu N and β tubulin III markers was determined using immunocytochemistry technique. Finally, all data were analyzed using the SPSS software.
Results:
Flow cytometry and immunocytochemistry results indicated that human dental pulp-derived stem cells were CD90, CD106-positive, but were negative for CD34, CD45 markers (
P
≤ 0.001). In addition, the mean percentage of β tubulin positive cells in different groups did not differ significantly from each other (
P
≥ 0.05). Nevertheless, the mean percentage of Neu N-positive cells was significantly higher in differentiated cells with embryoid bodies' source, especially in the presence of SHH than other groups (
P
≤ 0.05).
Conclusion:
It is concluded that due to the wide range of SHH functions and the facilitation of intercellular connections in the hanging droop method, it is recommended that the use of hanging drop method and SHH factor can be effective in increasing the efficiency of cell differentiation.
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Original Article:
Effect of different laser treatments on the shear bond strength of zirconia ceramic to resin cement
Mahnaz Hatami, Mohammadhossein Lotfi-Kamran, Abdolrahim Davari, Meisam Molazem
Dent Res J
2021, 18:56 (19 July 2021)
DOI
:10.4103/1735-3327.321867
Background:
Several techniques such as sand blast, silicoating, and laser irradiation have been introduced for reliable bond between zirconia and resin cement. This study aimed to assess and compare the effect of three types of lasers on the shear bond strength (SBS) of zirconia to resin cement.
Materials and Methods:
In this
in vitro
study, 55 zirconia disks (6 mm diameter × 3 mm thickness) were randomly divided into five groups: control (1), sandblast (2), carbon dioxide (CO
2
) (3), erbium-doped yttrium aluminum garnet (Er: YAG) (4), and neodymium-doped yttrium aluminum garnet (Nd: YAG) (5) laser irradiation. The surface morphology of one specimen from each group was evaluated by a scanning electron microscope. Zirconia disks were cemented to composite using Panavia F2. SBS test was performed at a crosshead speed of 1 mm/min after 24 h storage in distilled water and thermocycling. The data were analyzed by one-way analysis of variance and
post hoc
Tukey's HSD tests (α = 0.05).
Results:
The mean SBS values of the groups such as sandblast, Er: YAG, Nd: YAG, and CO
2
lasers and control were 6.64 MPa, 6.63 MPa, 4.98 MPa, 4.39 MPa, and 2.32 MPa, respectively. No significant difference was observed between sandblast and Er: YAG laser and between Nd: YAG and CO
2
lasers.
Conclusion:
All lasers increased SBS values of zirconia to resin cement in comparison to the untreated surface. Er: YAG laser was the most effective laser treatment on the bond strength equal to that of sandblast.
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Original Article:
A survey on osteogenic effect of collagen-membrane derived from
Rutilus kutum
swim bladder in rat calvaria
Farid Bahrizadeh, Hamidreza Azimi Lisar, Noushin Jalayer Naderi
Dent Res J
2021, 18:55 (19 July 2021)
DOI
:10.4103/1735-3327.321866
Background:
The collagen membrane which obtained from bovine pericardium and human skin in Guided Bone Regeneration (GBR) is costly and may even cause transmission of diseases. Replacing conventional collagen membranes with a more easily accessible and cheaper ones will have economic benefits. The aim was to determine the osteogenic effect of collagen-membrane derived from
Rutilus kutum
swim bladder on rat calvaria.
Materials and Methods:
The study was experimental. Thirty-six male albino rats of the Wistar strain were included in the study. The 5 mm surgical defects were created on calvarias and filled with allograft bone material and covered by
R. kutum
swim bladder (Group I), bovine derived pericardial membrane (Group II) and without membrane cover (Group III).The specimen were euthanized after 3, 5 and 8 weeks. The surrounding connective tissue was evaluated in term of osseous formation. Kruskal–Wallis, Univariant analysis of variance, and
post hoc
tests were used for statistical analysis. The
P
< 0.05 was considered statistically significant.
Results:
A significant differences between groups in terms of osseous formation (
P
= 0.001) was noted. The difference of osseous formation was significantly higher in 5 and 8 weeks than 3 weeks after operation in all groups (
P
= 0.03 and
P
= 0.006, respectively). The osseous formation in Group I and II were significantly higher than Group III (
P
= 0.023 and
P
= 0.001).
Conclusion:
The
R. kutum
swim bladder had osteogenic effect on rat calvaria.
R. kutum
swim bladder can be a new source in natural derived collagen membrane in GBR.
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Original Article:
The effect of 10% alpha-tocopherol solution and 5% grape seed extract on the microhardness and shear bond strength to bleached dentin
Manvi Srivastava, Ramakrishna Yeluri
Dent Res J
2021, 18:54 (19 July 2021)
DOI
:10.4103/1735-3327.321865
Background:
The aim of the study was to evaluate the effect of 10% alpha-tocopherol and 5% grape seed extract on the microhardness and shear bond strength (SBS) to bleached human dentin.
Materials and Methods:
This
in vitro
study was done on 200 extracted premolars which were decoronated and grinded to get flat dentin surface occlusaly. They were divided into four groups: (a) bleaching, (b) bleaching and application of alpha-tocopherol, (c) bleaching and application of grape seed extract, and (d) control. Groups were further subdivided into Subgroups I and II (
n
= 30) based on storage period before building with composite and were then tested for microhardness and SBS determination. The data thus obtained was subjected to statistical analysis which was performed using ANOVA test and
post hoc
Tukey's test. The significance for the entire statistical test was predetermined at
P
< 0.05.
Results:
The results showed that the microhardness values were minimum in Group A (immediately after bleaching) and maximum in control group. Comparison of data using one-way ANOVA showed that the
P
value was highly significant (
P
< 0.001) among the groups. The intergroup comparison of SBS using
post hoc
Tukey's tests revealed that the
P
value was significant (
P
< 0.05) when the comparison was done between the Group A and Group C and Group B with Group D immediately after bleaching.
Conclusion:
Adverse effects of bleaching can be reversed with the application of 10% alpha-tocopherol and 5% grape seed extract over the dentinal surface microhardness and SBS.
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Original Article:
Complications after dental rehabilitation under general anesthesia in Isfahan during February to May 2016
Maryam Ghafournia, Alireza Eshghi, Hamid Mosleh, Pedram Iranmanesh
Dent Res J
2021, 18:53 (19 July 2021)
DOI
:10.4103/1735-3327.321864
Background:
Children undergoing dental rehabilitations by general anesthesia (GA) commonly experience postoperative symptoms such as pain, fever, sore throat, and sleepiness. The aim of the present study was to investigate the specific complications of pediatric dental GA procedure.
Materials and Methods:
In this observational study sample included 72 children attending GA for dental treatment at the School of Dentistry, Isfahan University of Medical Sciences. Children with American Society of Anesthesiologists physical status I and without any communication or mental health problems were included. GA protocol was standardized. A number of complications were recorded by parents via filling a questionnaire for 2 days postoperatively. Data were analyzed using SPSS statistical software by Wilcoxon and Chi-squared test.
P
< 0.05 considered as significant level.
Results:
The most postoperative nonpsychological complications were dental pain (59.7 and 47.2% on days 1 and 2, respectively), followed by inability to eat normal (55.6 and 41.7% on days 1 and 2, respectively). All the patients' nonpsychological complaints had significantly decreased from day one to day two (
P
< 0.05). The most postoperative psychological complications were Attachments to parents (70.8 and 65.2% on days 1 and 2, respectively) followed by excessive crying (56.9 and 45.8% on days 1 and 2, respectively). All psychological complaints reduced by day two nonsignificantly except excessive crying which decreased significantly after 48 h (
P
= 0.004).
Conclusion:
The most postoperative complications after dental rehabilitation under GA were attachments to parents, dental pain, and inability to eat normal and excessive crying, respectively.
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Original Article:
Effect of various single file systems on microcrack formation in root canals: Scanning electron microscope study
Neelam Chandwani, Aastha Ranka, Ganesh R Jadhav, Dolly Jagyasi, Pooja Bopche, Arihant Golchha
Dent Res J
2021, 18:52 (19 July 2021)
DOI
:10.4103/1735-3327.321863
Background:
The aim of this study was to compare dentinal crack formation in root canal walls following 3 single file systems with continuous rotation under a scanning electron microscope (SEM).
Materials and Methods:
In this SEM study, seventy mandibular premolars were randomly divided into 5 groups. 3 experimental groups (
n
= 20) and 2 control groups (
n
= 5) as follows: Group I: Neolix NiTi file system, Group II: OneShape systems, Group III: OneCurve file system, positive control: conventional Hand File system, negative control: unprepared. After root canal preparations, the roots were sectioned at 3, 6, and 9 mm from the apex with water irrigation. The sections were inspected in all directions under SEM at × 100 magnification to determine the presence of cracks. The Chi-square test was used to analyze the data. There is a statistically significant difference in the crack formation between the apical third (
P
= 0.012) and coronal third (
P
= 0.002) when comparing all the 5 groups. No significant difference is found in the middle third (
P
= 0.46).
P
< 0.05 is considered statistically significant.
Results:
Maximum cracks in the apical third were seen with One Shape file 11 (55%) and in the coronal third with Neolix NiTi 14 (70%). There is a statistically significant difference in the crack formation only in OneCurve when comparing the apical, middle, and coronal third for the individual group (
P
= 0.042).
Conclusion:
There was a significant difference in crack formation in apical and coronal third. OneCurve caused the least incidence of cracks when compared to other file systems. OneCurve file system can be a choice for canal preparation over Neolix Niti and OneShape.
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Original Article:
Abrasiveness and whitening effect of charcoal-containing whitening toothpastes in permanent teeth.
Masoud Fallahinejad Ghajari, Maryam Shamsaei, Kimia Basandeh, Maedeh Sadeghpour Galouyak
Dent Res J
2021, 18:51 (19 July 2021)
DOI
:10.4103/1735-3327.321862
Background:
Charcoal toothpastes can whiten teeth through abrasion. The purpose of this study was to determine the level of whitening and abrasiveness of charcoal toothpastes in permanent teeth.
Materials and Methods:
In this
in vitro
study, 30 premolars were polished, sectioned, mounted, and stored for 5 days in a coffee solution at 37°C. The color and surface profile of the teeth were measured by spectrophotometry and a profilometric device, respectively. The specimens were divided into 3 groups of 10 and were brushed 2000 times (equivalent to 3 times a day for 1.5 months) in a brushing machine using 20 g of each toothpaste (Bencer, Beverly, and Colgate) mixed with 40 ml of distilled water. The color and surface profile were remeasured. Bonferroni test and repeated measures analysis of variance (ANOVA) were used to examine the abrasion. One-way ANOVA was used to assess the whitening.
Results:
The three toothpastes caused changes in the surface profile (
P
= 0.0001). ΔE was equal to 3.3 (within the acceptable range) in all groups (95% confidence interval). There was no significant difference in abrasion (
P
> 0.05) and color change (
P
= 0.884) among toothpastes.
Conclusion:
The results of this study showed that all the three used toothpastes have the abrasive and whitening effect on the samples significantly. The differences between the toothpastes were not significant.
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Original Article:
Evaluation the relationship between psychological profile and salivary cortisol in patients with recurrent aphthous stomatitis
Marjan Mirzaei, Mahdieh Zarabadipour, Monirsadat Mirzadeh
Dent Res J
2021, 18:50 (22 June 2021)
DOI
:10.4103/1735-3327.318945
Background:
Recurrent aphthous stomatitis (RAS) is one of the most common oral mucosal diseases which are diagnosed with recurrent and painful ulcers. The possible association between psychological factors and salivary stress related factors in patients with aphthous ulcers has been discussed in various studies. The aim of this study was to evaluate salivary cortisol level, anxiety, and depression in patients with RAS.
Materials and Methods:
In this case control study, 30 patients with RAS and 30 healthy individuals were enrolled by matching their age and sex. Anxiety and depression were assessed by beck anxiety inventory and beck depression inventory. Unstimulated saliva of both groups were collected and then tested by DiaMetra kit and the ELISA method. Data were analyzed by SPSS using an Independent
t
-test and Mann–Whitney test. A statistical significance level of <0.05 was considered.
Results:
The mean salivary cortisol in the case group was 5.35 ng/ml and in the control group was 4.73 ng/ml which was not statistically significant (
P
> 0.05). There was no significant difference in anxiety and depression level between the two groups (
P
> 0.05). According to Spearman correlation coefficient, there was an average, negative, and significant correlation between salivary cortisol and anxiety and depression scores in the case group (
P
< 0.05).
Conclusion:
This study shows that, although the mean salivary cortisol was slightly higher in patients with aphthous lesions, anxiety, and depression were not possible factors for RAS. There was a moderate, negative correlation between salivary cortisol level and stress and anxiety in patients with RAS.
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Original Article:
A comparative evaluation of the effect of three different concentrations of in-office bleaching agents on microhardness and surface roughness of enamel – An
in vitro
study
Khushboo Goyal, Suparna Ganguly Saha, Anuj Bhardwaj, Mainak Kanti Saha, Kaustubh Bhapkar, Shrija Paradkar
Dent Res J
2021, 18:49 (22 June 2021)
DOI
:10.4103/1735-3327.318944
Background:
To evaluate the changes in the micro-hardness and surface roughness of enamel treated with three different concentrations of in-office bleaching agents.
Materials and Methods:
In this
in vitro
study, 60 human incisors were divided into two groups (Group A and Group B). To obtain the baseline values, a Vickers tester was used to determine the surface microhardness in Group A, and a Surtronic tester was used for evaluation of surface roughness in Group B. Each group was then further subdivided into three subgroups and subjected to bleaching with Dash (Groups A1 and B1), Pola Office (Groups A2 and B2), and Opalescence Boost (Groups A3 and B3) containing 30%, 35%, and 40% hydrogen peroxide (HP), respectively. Samples were again subjected to testing to obtain the postbleaching values. Pre- and postbleaching data were analyzed by paired
t
-test. Intergroup comparison was carried out using one-way ANOVA (
P
≤ 0.05).
Results:
A significant decrease in microhardness values was observed following bleaching in all the three groups, with Group A1 showing maximum percentage decrease (2.58%), followed by Group A2 (1.23%) and Group A3 (0.73%). Furthermore, an increase in surface roughness was observed following bleaching, with Group B1 showing maximum percentage increase (14.80%), followed by Group B2 (8.25%) and Group B3 (5.79%). However, there was no significant difference in either microhardness or surface roughness when comparing the postbleaching values among the three bleaching agents.
Conclusion:
In-office bleaching agents may adversely affect the microhardness and roughness of enamel surface which are not related to the concentration of HP used.
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Original Article:
Microstructural, microchemical, and mechanical changes associated with the clinical reuse of two nickel–titanium endodontic instruments
Felipe Augusto Restrepo-Restrepo, Viviana Andrea Holguín-Vásquez, Syldana Julieth Cañas-Jiménez, Paula Andrea Villa-Machado, Sara Ochoa-Soto, Claudia Patricia Ossa-Orozco, Sergio Iván Tobón-Arroyave
Dent Res J
2021, 18:48 (22 June 2021)
DOI
:10.4103/1735-3327.318943
Background:
Nickel–titanium (NiTi) instruments have represented a great technological development that enabled endodontists conforming irregular-shaped root canals. Notwithstanding, the repeated use of these instruments may lead to the fracture without any prior visible warning signs. This study aimed to evaluate how multiple clinical instrumentation/sterilization cycles of two NiTi mechanized instruments can affect their microstructural, microchemical, and mechanical characteristics.
Materials and Methods:
In this observational descriptive study, a total of 140 NiTi instruments, 70 ProTaper Gold
®
(PTG) and 70 WaveOne Gold
®
(WOG) were analyzed. For each brand system, instruments were evaluated in the as-received condition (
n
= 10) and after one (
n
= 20), two (
n
= 20), and three (
n
= 20) instrumentation/sterilization cycles. Intraoperative instrumentation parameters were recorded for all used instruments. Afterward, the files were examined using scanning electron microscopy and energy-dispersive X-ray microanalysis. All of the instruments were tensile-fatigue tested until rupture in order to calculate the mechanical tensile strength and the maximum elongation percentage for the samples. Statistical analysis was completed using Chi-square, Kruskal–Wallis
H-
, or Mann–Whitney
U
-tests with a statistical significance set at
P
< 0.05.
Results:
Significant increasing changes in surface topography (
P
< 0.05, Chi-square test) and chemical composition (
P
< 0.05, Kruskal–Wallis
H
-test) in both brand systems through instrumentation/sterilization cycles were detected. In addition, values of mechanical tensile strength and maximum elongation percentage increased significantly through instrumentation/sterilization cycles in the PTG group, whereas only the median values of mechanical tensile strength increased significantly in the WOG group (all
P
< 0.01, Kruskal–Wallis
H
-test).
Conclusion:
Although multiple instrumentation/sterilization cycles may render NiTi instruments more flexible and fatigue resistant, the significant changes detected in their surface topography and chemical composition should preclude their repeated clinical use in the routine endodontic practice as prevention for breakage.
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Review Article:
An overview of surgery-first orthognathic approach: History, indications and limitations, protocols, and dentoskeletal stability
Atefe Ahmadvand, Shiva Alavi, Saeed Hasani Mehraban
Dent Res J
2021, 18:47 (22 June 2021)
DOI
:10.4103/1735-3327.318942
The most common orthognathic surgery approach consists of three steps: presurgical orthodontic treatment, surgery, and postsurgical orthodontic treatment. Despite its advantages, this technique has some disadvantages, too, including a long treatment period, problems in mastication and articulation, temporary worsening of the patient's appearance, and psychological problems for the patient. The introduction of the surgery-first orthognathic approach has been an attempt to overcome these problems. In this article, we wish to provide a comprehensive overview on this approach. In this approach, which consists of surgery without orthodontic preparation and a short period of orthodontic treatment after it, the overall duration of treatment decreases and the patient's appearance improves. The skeletal anchorage, placed at the time of surgery, can be used to facilitate tooth movements after surgery. Despite the advantages of this technique, it is associated with some limitations; in particular, occlusion cannot have a guiding role during surgery. Therefore, correct diagnosis, prediction of the outcomes, and simulating correction with the model setup are of crucial importance. The surgeon's knowledge and expertise have a significant role in this respect.
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Original Article:
Evaluation of periostin levels in gingival crevicular fluid in association between coronary heart disease and chronic periodontitis
Babak Rezaei, Mojtaba Bayani, Mehdi Anvari, Pedram Vahed
Dent Res J
2021, 18:46 (22 June 2021)
DOI
:10.4103/1735-3327.318941
Background:
Periostin is a protein, which is essential for periodontal tissue integrity, development and maturity. The aim of this study was to evaluate the role of gingival crevicular fluid (GCF) periostin levels in the association between coronary heart disease (CHD) and chronic periodontitis (CP).
Materials and Methods:
This matched case–control study was conducted on 116 participants. The participants were matched for age, gender, and body mass index and divided into four groups as follows: (1) 29 patients with CHD and sever CP (CHD-CP), (2) 29 patients with CHD and without CP (CHD-H), (3) 29 patients without CHD and with sever CP (H-CP), and (4) 29 healthy participants (H-H). The GCF periostin was collected and evaluated using the enzyme-linked immunosorbent assay (ELASA). Finally, the data were analyzed by analysis of variance using the stata software. Significance was assigned at
P
< 0.05.
Results:
The results showed that there was a significant difference in the GCF periostin levels in the four groups (
P
< 0.05). Moreover, according to the results of the Bonferroni's test, differences in the mean periostin levels were significant (
P
< 0.001) between CHD-CP and CHD-H, CHD-CP and H-CP, CHD-CP and H-H, CHD-H and H-H, and also between H-CP and H-H.
Conclusion:
The periostin levels reduced in the CHD patients, especially in the CHD-CP group. The findings reveal a probable role of periostin in the association between CHD and CP.
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Original Article:
Effect of indirect ultrasonic activation of modified bioceramic materials on the bond strength and tubular penetration in root canals
Bandaru Yamini, Praveen Kumar Gali, Bolla Nagesh, Sujana Varri, Roopadevi Garlapati, K Madhu Kiran Naik
Dent Res J
2021, 18:45 (22 June 2021)
DOI
:10.4103/1735-3327.318940
Background:
To maintain the integrity of the interface between root canal filling and radicular dentin an ideal endodontic biomaterial should have good adhesion. This study was aimed to evaluate the bond strength and tubular penetration of modified bioceramic materials by indirect ultrasonic activation.
Materials and Methods:
In the present
in vitro
experimental study, 120 coronal root slices of 2 mm were prepared from mandibular premolars and randomly divided into six groups (
n
= 20) in accordance to placement techniques: Group I: Nano Biodentine-manual, Group II: CaCl
2
modified ProRootMTA-manual (MM), Group III: Biodentine-manual, Group IV: Nano Biodentine: Ultrasonic, Group V: CaCl
2
modified ProRootMTA-ultrasonic, and Group VI: Biodentine-ultrasonic (BDU). The samples were kept in humidifier for 4 days at 37°C and push out bond strength, sealer penetration were evaluated using an universal testing machine and confocal laser scanning microscope respectively. Data were subjected to statistical analysis using SPSS software by using One-way ANOVA for overall significance and Tukey's multiple
post hoc
test for intergroup comparison (
P
< 0.05).
Results:
Highest push out bond strength and greater tubular penetration were observed with Group VI (BDU), while the lowest bond strength and tubular penetration were associated with Group II (MM).
Conclusion:
Within the limitations of current study it was observed that Biodentine with indirect ultrasonic activation has resulted in highest pushout bond strength among all the study groups.
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Original Article:
A multilevel analysis of a randomized clinical trial comparing adjunctive moxifloxacin versus amoxicillin/metronidazole for the treatment of aggressive periodontitis
Carlos-Martín Ardila, Jader-Alexander Bedoya-García
Dent Res J
2021, 18:44 (22 June 2021)
DOI
:10.4103/1735-3327.318939
Background:
It was documented that the clinical outcomes of mechanical periodontal treatment can fluctuate not merely concerning patients but equally among various tooth sites in the subject. This trial evaluates the clinical parameters related with the patient, tooth, and site that generate more changes in clinical attachment level (CAL) gain and probing depth (PD) reduction, using moxifloxacin (MOX) versus amoxicillin plus metronidazole (AMOX + ME) as adjuncts to scaling and root planing (SRP), in comparison to SRP only, post-therapy in generalized aggressive periodontitis (GAgP).
Materials and Methods:
The analysis of this clinical trial included 6012 tooth sites at 1002 teeth in 36 patients; they were randomly assigned to three protocols: Systemically intake of MOX or AMOX + ME plus SRP, or SRP + placebo for 7 days. The clinical effect of the patient, tooth, and site characteristics, in terms of CAL gain and PD reduction, was explored using a multilevel linear model.
P <
0.05 was statistically significant.
Results:
Following 6 months of treatment, the differences between the groups were statistically significant, favoring the MOX and AMOX + ME protocols (
P
< 0.0001). Moreover, the multilevel model showed that adjunctive MOX, AMOX + ME, non-molar, and interproximal sites were the features that contribute significantly to CAL improvement, and PD decreases in GAgP (
P
≤ 0.001 for all).
Conclusion:
The most relevant characteristics for the changes in CAL increase and PD diminution, after adjunctive antimicrobials, were ascribable to the features related to the tooth. MOX and AMOX + ME, non-multi-radicular-tooth, and interdental sites indicated superior clinical gains at the tooth and site levels in GAgP.
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Original Article:
Effect of systemic administration of omeprazole on osseointegration around titanium dental implants: A histomorphometric study in dogs
Negar Kanounisabet, Ahmad Mogharehabed, Nakisa Torabinia, Seyedeh Marzieh Hashemi Nejad, Jaber Yaghini
Dent Res J
2021, 18:43 (22 June 2021)
DOI
:10.4103/1735-3327.318938
Background:
Dental implants are widely accepted substitutes for replacing missing teeth. Many factors, including the use of specific drugs such as proton-pump inhibitors (PPIs) (omeprazole), can affect the success of dental implantations. The aim of this study was to investigate the relationship between the use of omeprazole and osseointegration of dental implants.
Materials and Methods:
This experimental animal study was performed on eight native male dogs weighted 11–13 kg and aged 16–20 months. The dogs were divided into two groups (receivers and nonreceivers of omeprazole). After extraction of mandibular teeth, treatment was started randomly with the administration of omeprazole and saline. After a 2-month recovery period, six titanium implants were placed in the jaws of all dogs and the administration of omeprazole was continued for 2 weeks. After 4 and 12 weeks, the dogs were anesthetized and dental implants with their bone marrow were removed. The samples were examined histomorphometrically to determine osseointegration. Data were analyzed with two-way ANOVA test for 95% confidence interval. The
P
value was set at 0.05.
Results:
In the microscopic examination of the samples in week 4, the levels of bone–implant contact (BIC) in the study group were significantly lower than the control group (46.37 vs. 64.37%). In 12 weeks, BIC was significantly lower than that of the control group (67.33 vs. 82.00%). The type of bone formed in week 4 in both the groups was more woven, and in the 12th week, it was mostly lamellar.
Conclusion:
Systemic administration of PPIs may interfere with osseointegration of dental implants.
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Original Article:
Prevalence of middle mesial canal in mandibular first molars using cone-beam computed tomography technique
Mohsen Hasheminia, Hamid Razavian, Ladan Khorrami, Hamid Mosleh, Sanaz Mohamadi
Dent Res J
2021, 18:42 (22 June 2021)
DOI
:10.4103/1735-3327.318937
Materials and Methods:
In this descriptive study, a total of 768 radiographs of mandibular first molars, 384 for males and 384 for females, taken at a private radiology center in Isfahan, were assessed for the presence of middle mesial canal based on the gender of the patients. All samples had been prepared by one CBCT machine. The images were evaluated by endodontists and radiologists. Then, the morphology of first molar mesial root canals was assessed by Vertucci classification, and the obtained results were analyzed by SPSS software using a Chi-square test. The comparison was considered statistically significant at
P
< 0.05.
Results:
Twenty-four samples (3.13%) had a middle mesial canal. The prevalence rates of the middle mesial canal in the females and males were found to be 9 and 15 (2.35% vs. 3.92%), respectively, indicating no statistically significant difference (
P
= 0.21). Of 24 teeth with middle mesial canal, 17 samples (70.8%) were of Type XII and 7 samples (29.2%) were of Type VIII. There was no statistically significant difference between genders with regard to the prevalence of different types of middle mesial canals (
P
= 0.19).
Conclusion:
The prevalence of the middle mesial canal was rather low in this study, and it is necessary to detect additional canals in the patients under root canal treatment.
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Review Article:
A systematic review and meta-analysis of failure to take history as a barrier of reporting child abuse by dentists in private and state clinics
Firoozeh Nilchian, Mohammad Javad Tarrahi, Narges Zare
Dent Res J
2021, 18:41 (22 June 2021)
DOI
:10.4103/1735-3327.318936
Background:
Since the symptoms of child abuse and neglect often manifest in the orofacial region, the dental team has a key role in identifying children subjected to abuse. This study was aimed to explore the prevalence of failure to take history as a barrier to reporting child abuse by dentist around the world.
Materials and Methods:
In this systematic review and meta-analysis, PubMed, Embase, Scopus, Google Scholar, ProQuest, Cochrane, and ISI databases were searched for the cross-sectional articles in English languages on barriers to reporting child abuse and lack of knowledge about referral procedures by dentists since 1985 using Medical Subject Headings (MeSH). The full-texts of all included articles were obtained and assessed for quality according to Newcastle–Ottawa Scale adapted for cross-sectional studies. The qualified articles were then studied thoroughly and results were extracted. Data were analyzed by Comprehensive Meta-Analysis software using meta-analysis and random effects model. Heterogeneity was determined by Q-test and I-square index.
Results:
A total of 17 articles were included in the meta-analysis. The prevalence of lack of knowledge about referral procedures as a barrier was determined according to the meta-analysis of the number of relevant articles and was (55%, confidence interval: 0.48, 0.62).
Conclusion:
The analysis of various studies revealed lack of information about referral procedures as an important barrier to report child abuse by dentists.
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Original Article:
Anatomy assessment of permanent mandibular premolar teeth in a selected Iranian population using cone-beam computed tomography
Seyed Mohsen Hasheminia, Mojdeh Mehdizadeh, Shervin Bagherieh
Dent Res J
2021, 18:40 (24 May 2021)
DOI
:10.4103/1735-3327.316657
Background:
Appropriate cleaning and shaping and three-dimensional obturation of the root canal system lead to a successful endodontic treatment. To achieve this, complete knowledge of the internal anatomy of root canals is necessary. Therefore, this study evaluated the number and shape of mandibular premolar roots and canals and classified their Vertucci classification using cone-beam computed tomography (CBCT) images of an Iranian sample population.
Materials and Methods:
This
in vivo
study was performed on CBCT images of patients aged 20–70 years who had attended a dental radiology center in Isfahan. CBCT images were examined in coronal, sagittal, and especially axial dimensions. Descriptive statistics were calculated. Groups were compared using the Chi-square of the Statistical Package for the Social Sciences. The level of significance was predetermined as 0.05.
Results:
In both first and second premolars, the most common classes were Type I followed by Type V. The Chi-square did not show significant differences between males and females in terms of Vertucci classes in the first (
P
= 0.305) or second premolar (
P
= 0.315).
Conclusion:
Since a thorough knowledge of root canal anatomy is necessary for successful root canal treatment and almost one out of ten mandibular premolars has additional canal in their root canal system, accurate evaluating of preoperative radiographs, taking CBCT images if necessary, and probing the root canal system with fine and precurved files should be done to negotiate the entire canal system.
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Original Article:
The effect of adjunctive use of melatonin as a supplement on serum ferritin level in periodontal patients: A randomized, controlled trial
Masoumeh Faramarzi, Mehrnoosh Sadighi, Mohammadtaghi Chitsazi, Mahdieh Esmailzadeh, Ghazale Foladvand
Dent Res J
2021, 18:39 (24 May 2021)
DOI
:10.4103/1735-3327.316656
Background:
Ferritin is an acute-phase protein that is increased in inflammatory diseases. Melatonin has been studied due to its antioxidant effects and the elimination of free radicals. The aim of this study was to evaluate the effect of melatonin supplement as an adjunct to routine periodontal treatment on serum ferritin levels in patients with periodontitis.
Materials and Methods:
Forty patients with chronic periodontitis were included in this randomized controlled clinical trial study. Twenty patients received nonsurgical periodontal treatment and twenty patients received nonsurgical periodontal treatment with adjunctive use of melatonin. Serum ferritin concentrations and periodontal parameters were recorded at baseline and 3 months after periodontal therapy. Serum ferritin level and periodontal parameters comparison at baseline and 3 months after periodontal treatment was done by Wilcoxon signed-rank test and paired sample
t
-test, respectively. Differences between test and control groups were evaluated by Mann–Whitney U-test for ferritin level and independent
t
-test for periodontal parameters, and a
P
≤ 0.05 was considered statistically significant.
Results:
Serum ferritin level decreased in both the control and test group in 3 months follow-up compared to baseline (
P
< 0.001). The reduction in ferritin levels in the test group was more than the control group, but this difference was not statistically significant (
P
= 0.414). Improvement in periodontal parameters were not significant between two groups (
P
= 0.489), but improvement rates in the test group were more than control.
Conclusion:
Melatonin, as an adjunct to periodontal therapy, showed additional benefits in the reduction of serum ferritin levels and improvement of periodontal parameters.
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Original Article:
Effects of Sabbagh Universal Spring 2 appliance on cephalometric indices in patients with Class II division 1 versus division 2 malocclusions: A preliminary before–after clinical trial
Siamak Hemmatpour, Parastesh Molladavoodi, Fatemeh Kadkhodaei Oliadarani, Hossein Bahrani, Vahid Rakhshan
Dent Res J
2021, 18:38 (24 May 2021)
DOI
:10.4103/1735-3327.316655
Background:
Since there is no comparison between the effects of Sabbagh Universal Spring 2 (SUS2) appliance on Class II division 1 (div 1) versus Class II division 2 (div 2) patients, this preliminary study was conducted to comparatively assess, for the first time, the effects of SUS2 on 34 cephalometric indices in Class II/1 versus Class II/2 patients.
Materials and Methods:
This before–after clinical trial was conducted on 75 observations of 25 patients with Class II malocclusion, of whom 12 (9 females and 3 males) had Class II div 1 and 13 (11 females and 2 males) had Class II div 2 malocclusion diagnosed by clinical examination and cephalometric assessment. The growth level of all patients had to be CS3 according to the cervical vertebral maturation index. Lateral cephalographs were obtained before treatment (T0) and the patients underwent fixed orthodontic treatment. Lateral cephalographs were taken again (T1) and the patients received SUS2 functional appliance for 6 months. A final lateral cephalograph (T2) was then obtained. Thirty-four dentoskeletal indices were measured on lateral cephalographs, and changes in indices over time were determined and compared using repeated-measures analysis of variance,
post hoc
test, and
t
-test (α =0.05).
Results:
Within-group comparisons showed significant changes over time in SNB, sella nasion (SN)/occlusal plane, ANB, articular (Ar)-pogonion (Pog), L1-NB, condylion (Co)-gnathion , S-Ar/Ar-G, B-RL1, L1/nasion-point B (NB), U6-RL2, incisor mandibular plane angle, overjet, overbite, U1-RL1, and L1-RL2 (
P
< 0.05). The two groups were significantly different in terms of changes occurred to overjet, interincisal angle, U1/RL1, L1-NB, U1-NA, U1/NA, the Jarabak ratio, A-RL1, U1/SN, Pog-NB, and Co-A (
P
< 0.05).
Conclusion:
The SUS2 showed therapeutic efficacy for both Class II div 1 and 2 patients although it more commonly caused dentoalveolar rather than skeletal changes. Our study showed no considerable difference between the two groups after using this appliance, and the difference in the Jarabak ratio indicated better long-term growth pattern of div 2 patients and its conformity with camouflage treatments (mild or moderate Class II).
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Original Article:
Antibacterial effectiveness of diluted preparations of intracanal medicaments used in regenerative endodontic treatment on dentin infected by bacterial biofilm: An
ex vivo
investigation
Mehrfam Khoshkhounejad, Mohammadreza Sharifian, Hadi Assadian, Mahsa Sobhi Afshar
Dent Res J
2021, 18:37 (24 May 2021)
DOI
:10.4103/1735-3327.316654
Background:
Conventional drug mixtures used in regenerative endodontic procedures have a toxic effect and no consensus has been reached about their best composition and concentration. Therefore, the aim of this study was to determine minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and minimum biofilm inhibitory concentration (MBIC) of the antimicrobial preparations and to compare their antimicrobial efficacy on bovine dentin infected by
Enterococcus faecalis
.
Materials and Methods:
For this original
ex vivo
investigation, prepared concentrations (MIC, MBC, and MBIC) of triple antibiotic paste (TAP), double antibiotic paste (DAP), modified triple antibiotic paste (MTAP)-1, MTAP2, co-amoxiclav, and calcium hydroxide (CH) were added to the prepared bovine dentin blocks (which incubated in
E. faecalis
suspension previously) and incubated for 3 days. The samples were subsequently prepared for culture and CFU counts. Statistical analysis of data was carried out using one-way analysis of variance and
post hoc
tests. The statistical power was set at
P
< 0.05.
Results:
All medicament groups significantly showed an antimicrobial efficacy compared with negative control (without antibiotic) (
P
< 0.001). TAP, DAP, co-amoxiclav, and CH (at its MBC value) were significantly capable of eliminating
E. faecalis
biofilm and showed no significant difference in comparison with positive control (complete biofilm removal) (
P
< 0.05).
Conclusion:
TAP, DAP, co-amoxiclav, and CH (at its MBC value) could effectively eliminate biofilm bacteria on the dentin surface. Antimicrobial efficacy of other medicaments containing cefaclor or clindamycin was limited.
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Original Article:
Immunohistochemical evaluation of myofibroblasts in odontogenic keratocyst, dentigerous cyst and different clinical variants of ameloblastoma: A comparative study
VV Anusai, S Shylaja, M Suvarna, OV Ramanand, E Sharath Reddy, V Raghu Vamshi
Dent Res J
2021, 18:36 (24 May 2021)
DOI
:10.4103/1735-3327.316653
Background:
Odontogenic cysts and tumors exhibit varying degrees of aggressiveness in their biological behavior. Odontogenic keratocyst (OKC), dentigerous cyst (DC), ameloblastoma are most common odontogenic cysts and tumors to occur in the oral cavity. Myofibroblasts (MFs) in the connective tissue stroma participate in the matrix degradation process by secreting matrix metalloproteinase 2, transforming growth factor beta1 and may contribute to variation in their biological behavior. Its activity is identified by alpha-smooth muscle actin (α-SMA) marker. With this background, the present study aims to evaluate the frequency of MFs using α-SMA to determine the biological behavior of OKC, DC, and different clinical variants of ameloblastoma.
Materials and Methods:
A retrospective study was carried out with total of 60 samples which include 10 cases each of OKC, DC, 30 cases of different clinical variants of ameloblastomas and 10 normal mucosa taken as controls. All are stained immunohistochemically using α-SMA and were analyzed for the same. Comparison between more than 2 groups done by one way analysis of variance test with the level of significance of
P
≤ 0.0001, i.e., <0.05.
Results:
Statistically significant difference in the mean number of MFs observed between certain groups, with higher mean number in solid ameloblastoma (SA) (32.45) followed by OKC (28.79), unicystic ameloblastoma (24.53), desmoplastic ameloblastoma (7.44), and DC (1.72).
Conclusion:
Higher frequency of MFs noticed in SA, OKC which are key cells for connective tissue remodeling by interacting with epithelial cells and other connective tissue cells to facilitate progression of cysts and tumors thereby contributing to their biological behavior.
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Original Article:
Efficacy of exenatide, ozone, and methyl prednisolone for the recovery of infraorbital nerve paresthesia after injury in rats
Mehdi Abrishami, Arash Golestaneh, Laleh Maleki, Hasan Momeni, Mazyar Manshaei
Dent Res J
2021, 18:35 (24 May 2021)
DOI
:10.4103/1735-3327.316652
Background:
Infraorbital nerve injury often occurs due to zygomatic complex fracture. There is no standard protocol to restore the sensory and motor function of the nerve after injury. Some medications are used to improve nerve function. This histopathological animal study aimed to assess the neurotrophic efficacy of exenatide, ozone, and methyl prednisolone for injured infraorbital nerve.
Materials and Methods:
In this animal study, 60 rats were randomly divided into five groups (
n
= 12) of control, saline, methyl prednisolone, exenatide, and ozone. Under general anesthesia, a unilateral infraorbital incision was made, and the nerve was compressed with a hemostat for 5 s. Depending on the study group, the rats received the designated medications. All rats were sacrificed after 14 days, and the nerve recovery was evaluated by observing the slides prepared from the area under a light microscope using histological and immunohistochemical parameters. Data were analyzed using Chi-square test and Tukey's least significant difference test (
P
< 0.05).
Results:
No inflammation in the peripheral tissue had a significantly higher frequency in the methyl prednisolone group (
P
< 0.05). Degeneration, intracellular inflammatory infiltrate, muscle injury, fibrosis around the nerve, granulation tissue, vascular proliferation, and infiltration of inflammatory cells around the nerve were more commonly absent in the exenatide group (
P
< 0.05).
Conclusion:
Exenatide showed superior efficacy for the recovery of infraorbital nerve function after injury. Thus, it may be beneficial for postoperative care following infraorbital nerve injury.
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Case Report:
Persistence of postoperative pain due to extrusion of endodontic obturator plastic carrier: A report of two cases treated with a periradicular microsurgical approach
Silvio Taschieri, Gianluca Gambarini, Irina Makeeva, Svetlana Tarasenko, Stefano Corbella
Dent Res J
2021, 18:34 (24 May 2021)
DOI
:10.4103/1735-3327.316651
The aim of the present study was to describe two clinical cases showing postoperative pain associated with the use of plastic carrier obturation system and apical bone fenestration. The patients were treated by surgical access and apicoectomy through a modern technique (using magnification and microsurgical approach), thus removing the direct contact between obturation material and submucosal connective tissue. The surgical interventions were carried on without the occurrence of any complication. Postsurgical adverse sequelae were negligible. After few weeks from the surgery, all symptoms disappeared. Radiographic healing was observed after 48 months. The presence of apical bone fenestration could be the cause of persistent pain after root canal treatment. The contact between plastic carrier and submucosal connective tissue could be the direct cause of spontaneous pain even in absence of periapical infection. Since the clinical diagnosis could be difficult, the use of tridimensional radiology could be justified. Surgical approach, by the removal of the contact between the carrier and connective tissues, can be considered a viable option to treat these particular affections.
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Original Article:
Efficacy of silver nanoparticle gel on healing of traumatic oral ulcers compared with triamcinolone oral paste: An experimental study on rats
Fahimeh Rezazadeh, Ali Dehghani Nazhvani, Samira Sadat Abolmaali, Fatemeh Dehghani Nazhvani, Alireza Bonyani
Dent Res J
2021, 18:33 (24 May 2021)
DOI
:10.4103/1735-3327.316650
Background:
There are many treatment protocols for relieving pain and accelerating oral ulcer healing. The purpose was to evaluate the anti-inflammatory and antimicrobial effect of silver nanoparticle (AgNP) gel on oral ulcers compared with the traditional treatment in animal model.
Materials and Methods:
In this experimental study, 72 adult male rats were randomly allocated into four groups, after ulcer induction: control (receive no treatment), placebo (placebo gel was applied on ulcers once daily), triamcinolone (ulcers were treated with triamcinolone paste once daily), and AgNPs (ulcers were treated with AgNP gel once daily). The animals were sacrificed at three successive periods of 3, 7, and 14 days after ulcer induction. Histopathological scores of re-epithelialization and degree of inflammation were assessed. The wound size was measured clinically. For parametric data, the analysis of variance, and for ordinal histopathological data, the Kruskal–Wallis test using SPSS was done. A
P
< 0.05 was considered statically significant.
Results:
Control and placebo groups had no case of complete healing. Improvement of wound size by triamcinolone paste was not significant in different days but showed significant differences in degree of inflammation (
P
< 0.001) and re-epithelialization (
P
= 0.012). Reduction of wound size by AgNPs gel had significant difference at 14
th
day (
P
< 0.001); further, by this gel, significant differences in degree of inflammation and re-epithelialization were seen in long-term medication.
Conclusion:
AgNP gel could be effective in oral ulcer healing clinically and histopathologically. Although this gel showed delayed effect, it had better healing effect according to the cases with complete healing. Hence, the application of AgNP oral gel can be considered as an alternative approach for oral ulcers.
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Original Article:
Ions release evaluation and corrosion of titanium mini-implant surface in response to orthokin, oral B and chlorhexidine mouthwashes
Shiva Alavi, Atefe Ahmadvand
Dent Res J
2021, 18:32 (24 May 2021)
DOI
:10.4103/1735-3327.316649
Background:
The present study was performed to evaluate the effect of three types of mouthwash (orthokin, oral B and chlorhexidine [CHX]) on releasing of aluminum (Al), Titanium (Ti) and Vanadium (V) ions from titanium mini-implants (TMIs).
Materials and Methods:
In this
in vitro
, experimental study, a total of 40 TMIs were divided equally into four groups (10 TMI in each group) and then were immersed into Orthokin, Oral B, CHX, and artificial saliva, as a control. The experiments were performed for 21 days as following groups 1–7 days, 8–14 days, and 15–21 days. The inductively coupled plasma-optical emission spectrometry method was used to assess releasing metal ions after immersion in the storage media. In addition, before and after each experiment, the corrosion of TMIs was assessed using a scanning electron microscope (SEM). All results were analyzed using Kruskal–Wallis, followed by Bonferroni-adjusted Mann–Whitney
U
-test at 0.05 level of significance.
Results:
Our data showed that the maximum concentration of released Al was in the 1
st
week of exposure to Orthokin and Oral B (202.3 ± 68.5 and 72.3 ± 15.2 μg/L, respectively). Oral B exposure of TMI also caused to releasing of Ti to 128.1 ± 42.5, 54 ± 19.4 and 22 ± 6 μg/L for 1–7 days and 8–14 days and 15–21 days, respectively. Orthokin and CHX also induced the release of Ti more than artificial saliva (
P
< 0.05). In addition, there was no significant statistical difference between any types of mouthwashes and artificial saliva in releasing V. The results of SEM images also confirmed the corrosion effects of mouthwashes.
Conclusion:
The factors of exposure time and mouthwash type influenced the pattern of releasing Al and Ti as well as corrosion level.
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Original Article:
Effect of citric acid on force decay of orthodontic elastomeric chains
Amin Khaleghi, Atefe Ahmadvand, Saeid Sadeghian
Dent Res J
2021, 18:31 (24 May 2021)
DOI
:10.4103/1735-3327.316648
Background:
This study aimed to assess the effect of citric acid, as a weak acid commonly used in food industry, on elastomeric chain force decay.
Materials and Methods:
In this
in vitro
, experimental study, sixty elastomeric chains from two commercial brands of American Orthodontics and Ortho Technology (
n
= 30) were cut into five-piece segments. Elastomeric chains of each brand were randomly divided into two groups of control (artificial saliva) and citric acid. All elastomeric chains were incubated in artificial saliva at 37°C. Experimental samples were immersed in 10 mL of citric acid for 90 s daily and were then transferred back to the artificial saliva. The elastomeric chain force was measured at baseline (before the experiment), 1 day, 1 week, 2 weeks, and 3 weeks using an electromechanical universal testing machine. Data were analyzed using
t
-test, Kruskal–Wallis test, and Mann–Whitney U-test at 0.05 level of significance.
Results:
The elastomeric chain force gradually degraded over time. The difference in this respect was not significant at 2 and 3 weeks in any group (
P
> 0.05). On initiation of the experiment, the force in the citric acid group experienced a greater decay than that in the control group; the difference between the citric acid and control groups in both the brands was significant at all time points until the end of the 3
rd
week (
P
< 0.05). The difference between the American Orthodontics and Ortho Technology brands in the control and citric acid groups was significant at all time points (
P
< 0.001).
Conclusion:
Elastomeric chains in both the citric acid and artificial saliva groups experienced force decay over time. Force decay was greater in the citric acid group. Thus, citric acid can effectively decrease the elastomeric chain force. The Ortho Technology chain force was higher than that of American Orthodontics in both the groups at all time points.
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Original Article:
Flexural strength of polymethyl methacrylate reinforced with high-performance polymer and metal mesh
Zahra Hashemi Ardakani, Rashin Giti, Shima Dabiri, Amir Hashemi Hosseini, Marzieh Moayedi
Dent Res J
2021, 18:30 (24 May 2021)
DOI
:10.4103/1735-3327.316647
Background:
The flexural strength (FS) of a denture base material is of great concern, and many approaches have been used to strengthen the denture acrylic resins. The present study aimed to evaluate the effect of high-performance polymer (BioHPP) and metal mesh reinforcement on the FS of a heat-cured poly methyl methacrylate (PMMA) acrylic resin.
Materials and Methods:
This experimental study was done on 30 rectangular specimens (64 mm × 13 mm × 3 mm) of a heat-cured PMMA resin. The specimens were divided into three groups (
n
= 10) to be reinforced with either metal mesh or BioHPP mesh; one group was left nonreinforced, serving as the control group. The FS of specimens was assessed through a 3-point bending test by using a universal testing machine at a crosshead speed of 2 mm/min. Kruskal–Wallis H and Dunn's
post hoc
tests were used to compare the FS among the groups (alpha = 0.05).
Results:
The FS in the metal-reinforced group was statistically significantly higher than the two other groups (
P
< 0.001). However, the FS of the BioHPP-reinforced samples was not statistically significantly higher than the nonreinforced ones (
P
= 0.614).
Conclusion:
Reinforcing the PMMA with metal mesh significantly enhances its FS while BioHPP has no significant effect on the PMMA FS.
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Case Report:
A new classification of peri implant gaps based on gap location (A case series of 210 immediate implants)
Douglas Deporter, Ali Akbar Khoshkhounejad, Nikfam Khoshkhounejad, Mohammad Ketabi
Dent Res J
2021, 18:29 (6 April 2021)
DOI
:10.4103/1735-3327.313124
Based on our collective experiences with gap management around immediate dental implants, we have proposed a classification of gap type based on the location in relation to implant periphery. Seven types are proposed, and all but one type should heal without gap grafting provided that flap-less surgery and atraumatic extraction have been achieved. The exception is our Type II gap where the implant has been placed too far buccally leaving a gap only on the lingual/palatal. In this case, the lingual/palatal gap need not be grafted, but the buccal aspect of the implant should best to augmented to avoid the complications.
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Case Report:
A novel regenerative endodontic procedure in a traumatized immature tooth using amniotic membrane
Ellana Jermiah Joseph, Mahabala Y Karuna, Arathi Rao, Ashwin Rao, Anupama P Nayak
Dent Res J
2021, 18:28 (6 April 2021)
DOI
:10.4103/1735-3327.313123
Tooth nonvitality is one of the frequently seen consequences of dental trauma that causes the arrest of root development. Amniotic membrane has received a lot of attention for its use in transplantation and regeneration procedures. This article reports a unique and novel case of successful regenerative endodontic procedure done using amniotic membrane in a traumatized immature right maxillary central incisor of an 8-year-old girl. The clinical and radiographic evaluation done during the recall visits at 1, 3, 6, 9 and 12 months showed a progressive root growth with apical closure.
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Original Article:
Correlation between maxillary cuspid impaction with available space and anomalies of maxillary lateral incisors
Kimia Rohani, Ladan Eslamian, Golnaz Nahvi
Dent Res J
2021, 18:27 (6 April 2021)
DOI
:10.4103/1735-3327.313122
Background:
Association of cuspid impaction with adjacent lateral incisor anomalies is under controversy. The aim of this study was to investigate the correlation between maxillary cuspid impaction with dental anomalies.
Material and Methods:
In this
in vitro
experimental study, the material consisted of pretreatment dental records of 102 patients with at least one palatally or buccally displaced impacted permanent cuspid (palatal and buccal impaction groups). They were matched with a comparison control group of 102 patients having normally erupted maxillary cuspids. Available space, mesiodistal dimensions of teeth, and morphologic parameters of lateral incisors were measured using the digital caliper. Comparison of mean values of lateral incisors anomalies and severity of crowding between different groups were performed using the one-way ANOVA test, and the analysis of associations between position of the impaction and anomaly of the lateral incisors and severity of crowding was performed using the Chi-square test.
P
< 0.05 was considered statistically significant.
Results:
There was no statistically significant difference in the arch length–tooth size discrepancy between the cuspid impaction groups and control group. The impaction group (buccal or palatal) presented statistically significant differences in terms of anomalies of maxillary lateral incisor compared to the control group. Peg-shaped lateral incisor was related to buccal cuspid impaction and microdontia had relationship with palatal cuspid impaction.
Conclusion:
There is the relationship between cuspid impaction and adjacent lateral incisor abnormality, but no difference was observed between buccal and palatal cuspid impactions. Crowding revealed no relationship with cuspid impaction (buccal or palatal) in this study.
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Original Article:
Utilization of dental care in Iranian pregnant women: Findings from a population-based study
Donya Rahebi, Tannaz Valadbeigi, Jalil Hasani, Mahmoud Hajipour, Saeed Erfanpoor, Koorosh Etemad, Halime Yaghoobi, Fatemeh Zolfizadeh, Firooz Esmaeilzadeh, Ali Gholami, Abdolhalim Rajabi
Dent Res J
2021, 18:26 (6 April 2021)
DOI
:10.4103/1735-3327.313121
Background:
Although dental care attendance during pregnancy has been recommended by guidelines and institutions, the demand for dental services is still low among pregnant women. The aim of this study was to examine the prevalence of not receipt dental care and also determinants of that during pregnancy.
Materials and Methods:
This population-based study was conducted on 4071 mothers in 10 provinces of Iran, during 2014–2015. We calculated the prevalence of not receipt of dental care, and reasons for nonreceipt of care. We used logistic regression to estimate odds of nonreceipt of care by demographics variables. In the analyses, the level of statistical significance was set at
P
< 0.05.
Results:
Overall, 54.70% of women had no dental visit during pregnancy. In mothers who had a history of stillbirth, neonatal death and live birth, the prevalence of not receipt dental care during pregnancy were 54.56%, 48.92%, and 58.76%, respectively. The logistic regression analyses showed that parity second-to-fourth birth than first birth (odds ratio [OR] 1.37 confidence interval [CI] 95% 1.17–1.59, residence in rural (OR 1.68 CI 95% 1.45–1.95), and not intended pregnancy (OR 1.32 CI 95% 1.03–1.68) associated with not received dental care during pregnancy.
Conclusion:
Most pregnant women in this study received insufficient dental care. The need for dental care during pregnancy must be promoted widely among women of reproductive age, and family barriers to dental care should be addressed.
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Original Article:
Effect of accelerated aging and double application on the dentin bond strength of universal adhesive system
Mahmoud Bahari, Siavash Savadi Oskoee, Mohammad Esmaeel Ebrahimi Chaharom, Mehdi Abed Kahnamoui, Sarah Gholizadeh, Farnaz Davoodi
Dent Res J
2021, 18:25 (6 April 2021)
DOI
:10.4103/1735-3327.313120
Background:
Despite the many advantages of simplified adhesive systems, there are concerns about the durability of the adhesive layer over time. The aim was to investigate the effects of various aging methods and double application of an adhesive layer on the bond strength of the universal adhesive system using etch-and-rinse (ER) and self-etch (SE) strategies.
Materials and Methods:
In this
in vitro
study, the occlusal enamel of 120 extracted, intact human third molars was removed to expose the dentin. Then, the samples were randomly divided into four groups of thirty according to All-Bond Universal (ABU) adhesive application strategy (ER and SE) and the number of adhesive layers (1 or 2). Then, each group was subdivided into three subgroups of ten according to aging method (control, thermal cycling, and 10% sodium hypochlorite [NaOCl]). The shear bond strength was measured at the strain rate of 0.5 mm/min. Data were analyzed using three-way ANOVA and Tukey's
post hoc
tests (
P
< 0.05).
Results:
The effect of adhesive application strategy (
P
< 0.001) and aging method (
P
< 0.001) on the bond strength was statistically significant, but the effect of the double application was not statistically significant (
P
> 0.05). In addition, the interactive effect of adhesive application strategy–aging method was significant (
P
= 0.005).
Conclusion:
Using ABU with ER strategy leads to higher dentin bond strength compared to the SE method in the control and thermal cycling groups. However, no significant differences were observed between ER and SE strategies after aging with the NaOCl. Furthermore, the double application might not have any effect on the bond strength and durability.
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Original Article:
Ranking the soft skills of the dental profession based on the importance in job performance: A mixed method study in Isfahan and Mazandaran Universities of Medical Sciences
Roghayeh Valipour Khajeghyasi, Mohammad Javad Liaghatdar, Mohammad Reza Nili, Mandana Shirazi
Dent Res J
2021, 18:24 (6 April 2021)
DOI
:10.4103/1735-3327.313119
Background:
Soft skills are the key indicators of success and quality of performance in various professions, including the dental profession. The skills are the main tools for enhancing the quality of technical work in Dentistry. Hence, the aim of this study was to identify and prioritize the soft skills in dental profession.
Materials and Methods:
The study employed a mixed methods research. In the qualitative phase, the dental skills were identified using a semi-structured interview with faculty members and dental students. In the quantitative phase, the soft skills were prioritized with Analytical Hierarchical Process (AHP). The data analysis was using Expert Choice software.
Results:
The results showed that the dental profession to improve the quality of performance needs to 29 soft skills in six categories. In professional ethics, work ethics (with weight of 0.212) and responsibility (0.188); in artistic skills, handicrafts (0.616); in cognitive skills, decision-making ability (0.290) and spatial thinking (0.249); in communication skills, verbal/nonverbal communication and listening (0.266), and patience (0.240); in individual characteristics, stress management (0.381) and self-esteem (0.260); and finally, in management skills, time management (0.481) are among the most important soft skills in dental profession.
Conclusion:
Identify and prioritize the soft skills can help to improve the quality of performance the dental profession. Therefore, it is recommended to integrate these skills into the dentistry curriculum. Moreover, by increasing the number of studies the essential backgrounds for developing soft skills in dentistry education will be provided.
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Case Report:
Fabrication of a customized impression metal jig for an implant-supported overdenture with a milled bar
Yeganeh Memari, Azadeh Tadayonfard, Amir Fayaz, Elaheh Beyabanaki
Dent Res J
2021, 18:23 (6 April 2021)
DOI
:10.4103/1735-3327.313118
The use of computer-aided design/computer-aided manufacturing technology simplifies the laboratory and clinical steps for the fabrication of implant prostheses. It also reduces additional costs for the prosthetic components and technical procedures. This article describes a modified impression technique using an impression metal jig to enhance the accuracy of final impression and also to eliminate the need for the try-in of resin pattern of the milled bar.
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Original Article:
Microleakage of different temporary luting agents used for cementing provisional restorations on custom cast posts and cores
Maryam Mohajerfar, Naeemeh Nikfarjam Nouri, Tabasom Hooshmand, Elaheh Beyabanaki
Dent Res J
2021, 18:22 (6 April 2021)
DOI
:10.4103/1735-3327.313117
Background:
This study aimed to compare the effect of four temporary luting agents on preventing the coronal microleakage of teeth restored with custom cast post and core.
Materials and Methods:
In this
in vitro
original study, after removing 9 mm of gutta percha from root canals of 32 single-canaled premolars, the acrylic resin patterns of the post and core were fabricated. Patterns were cast with Ni-Cr metal alloy and then cemented with glass ionomer. Using self-curing acrylic resin, provisional restorations were made. Then, teeth were randomly allocated to four groups for each temporary cement including zinc oxide eugenol (Temp Bond), zinc oxide noneugenol (Temp Bond NE), noneugenol resin-based temporary luting agent (Temp Bond Clear), and noneugenol urethane methacrylate polymer-based temporary luting agent (Dento temp). Teeth were subjected to thermocycling, immersed in silver nitrate for 6 h and then sectioned. The amount of dye penetration in the margin of provisional crown and into the coronal part of the root canals was measured using a stereomicroscope. Data were analyzed using the Kruskal–Wallis and Dunn tests with significance level of 0.05.
Results:
All the luting agents showed some degree of microleakage (Group 1 showed the most microleakage). According to the pair comparison between the groups, Group 1 revealed a significant difference with others (
P
< 0.05). Furthermore, Group 2 showed more microleakage as compared to Group 4 (
P
= 0.037).
Conclusion:
Temp Bond showed the highest microleakage as compared to other luting agents. Furthermore, Temp Bond NE revealed a weaker sealability as compared to Dento temp.
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Original Article:
Surface characterization and frictional force between stainless steel brackets and archwires in orthodontic patients using chlorhexidine- and Persica-containing mouthrinses: A randomized controlled trial
Elham-Sadat Emadian Razavi, Tahereh Hosseinzadeh Nik, Tabassom Hooshmand, Habibeh Farazdaghi, Arian Hesam Arefi
Dent Res J
2021, 18:21 (6 April 2021)
DOI
:10.4103/1735-3327.313116
Background:
The aim of this study was to compare the effect of chlorhexidine- and Persica-containing mouthrinses on the surface characterization of orthodontic appliance and friction between the orthodontic stainless steel wires and brackets.
Materials and Methods:
In this randomized controlled trial, 75 orthodontic patients (aged 13–30) were allocated (
n
= 25) into two experimental groups (prescribed by Persica- or chlorhexidine-containing mouthrinse) and one control group (no prescription). The ovoid stainless steel archwires were placed, and the maxillary first premolar stainless steel edgewise brackets were ligated to wires by elastomeric rings. The patients were recalled after 2 weeks, and then, the archwires were removed and replaced. The surface analysis of archwires and brackets was evaluated using scanning electron microscopy and atomic force microscopy. The frictional forces between the archwires and brackets were measured using a universal testing machine. The data for surface roughness were analyzed by the nonparametric Kruskal–Wallis and Mann–Whitney tests. The frictional forces data were analyzed using a two-way analysis of variance and Tukey's
post hoc
test using SPSS software. The level of significance was
P
< 0.05.
Results:
The retrieved brackets showed minor changes in their surface topography. The surface roughness of archwires after the intervention was significantly greater for the chlorhexidine than that of Persica (
P
< 0.05). The friction force between the archwires and brackets was also significantly higher for the chlorhexidine than that of Persica (
P
< 0.05).
Conclusion:
The Persica-containing mouthrinse from the biomechanical and biochemical standpoints may be a better option for oral hygiene in orthodontic patients compared with the chlorhexidine.
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Original Article:
Effect of casein-phosphopeptide amorphous calcium phosphate and fluoride with/without erbium, chromium-doped yttrium, scandium, gallium, and garnet laser irradiation on enamel microhardness of permanent teeth
Arezoo Ghelejkhani, Sareh Nadalizadeh, Mansoureh Rajabi
Dent Res J
2021, 18:20 (17 March 2021)
DOI
:10.4103/1735-3327.311427
Background:
Laser therapy, along with the use of fluoridated compounds is a novel technique suggested for caries prevention. Casein phosphopeptide amorphous calcium phosphate (CPP-ACP) is another product suggested for this purpose. This study compared the effect of CPP-ACP and fluoride with/without Erbium, chromium-doped yttrium, scandium, gallium, and garnet laser irradiation on enamel microhardness of permanent teeth.
Materials and Methods:
This
in vitro
experimental study evaluated 35 extracted third molars. The teeth were decoronated, and the crowns were split into buccal and lingual halves. The samples were randomly divided into seven groups (
n
= 10) of GC Tooth Mousse, MI Paste Plus, laser, fluoride varnish, laser + GC Tooth Mousse, laser + MI Paste Plus, and laser + fluoride varnish. The baseline microhardness was measured before the intervention. After the intervention, the samples were kept in artificial saliva for 1 h and were then immersed in the demineralizing solution for 3 h followed by 21 h of immersion in the remineralizing solution for a total period of 12 days. Finally, the teeth were kept in the remineralizing solution for 2 more days. The secondary microhardness of the teeth was then measured. Data were analyzed using the Shapiro–Wilk test, two-way ANOVA, and Tukey's Honestly Significant Difference test.
Results:
The fluoride varnish (14.31%) and laser + fluoride varnish (18.79%) groups experienced minimum reduction in microhardness, while the GC Tooth Mousse group experienced maximum reduction in microhardness (91.64%) (
P
< 0.001). Laser irradiation before the application of remineralizing agents increased the microhardness only in laser + GC Tooth Mousse group (
P
< 0.001).
Conclusion:
Fluoride varnish increased the enamel microhardness, while GC Tooth Mousse had no such effect. Laser therapy before the application of remineralizing agents did not significantly enhance enamel resistance to demineralization.
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Original Article:
A new teaching model with artificial teeth containing simulated pulpal tissue
Hamid Razavian, Kimia Hanjani
Dent Res J
2021, 18:19 (17 March 2021)
DOI
:10.4103/1735-3327.311426
Background:
This study aimed to evaluate the use of artificial teeth for endodontic training for dental students.
Materials and Methods:
The following aspects of the artificial teeth were approved by the endodontists in Isfahan University of Medical Science: internal and external anatomy; coronal pulp chambers regarding their size, shape, and canal path; the root canals regarding their size, shape, and position; filling the pulp chamber and root canals by considering the texture, quantity, color, and ease of handling; and resin hardness and visualization of the radiographic image.
Results:
The results showed favorable opinions regarding the internal and external anatomy, coronal pulp chamber, root canal (s), handling, and radiographic imaging. The contents of the pulp space and hardness of the teeth were satisfactory.
Conclusion:
The artificial teeth tested have the potential to replace the natural teeth in preclinical training.
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Original Article:
Effect of coating on the color and surface hardness of the surface of dental ceramics
Esra Kul, Funda Bayindir, Pinar Gül, Ruhi Yesildal, Khamirul Amin Matori
Dent Res J
2021, 18:18 (17 March 2021)
DOI
:10.4103/1735-3327.311425
Background:
The aim of this study was to investigate whether boron nitride (BN) and BN–chromium (BN–Cr) coatings applied with the sputtering method would change the characteristics of hardness and discoloration of the ceramic veneer surface.
Materials and Methods:
In this experimental study, thirty disc-shaped cores (10 mm in diameter and 0.8 mm in thickness) were prepared. Three different ceramic systems, IPS Empress (E) (Ivoclar Vivadent, Schaan, Liechntenstein), IPS e.max Press (EP) (Ivoclar Vivadent, Schaan, Liechntenstein), and Turkom Cera (T) (Turcom-Ceramic SDN-BHD, Kuala Lumpur, Malaysia), were tested, each with
n
= 10. The specimens were divided into two subgroups (
n
= 5) according to the coating on the veneering ceramic: BN or BN–Cr. The Vickers hardness and color differences (ΔE) values were measured before and after coating. Surface analysis was performed with X-ray diffraction, X-ray photoelectron spectroscopy, and scanning electron microscopic. The Wilcoxon signed-rank test was performed to compare hardness values. The Kruskal–Wallis test was performed to compare ΔE values among all groups. The
post hoc
tests was used after Kruskal–Wallis, with level of statistical significance set at
P
≤ 0.05.
Results:
BN–Cr coating statistically significantly increased the hardness of all types of ceramics (
P
< 0.05). BN coating did not change the hardness statistically significantly (
P
> 0.05). The mean ΔE values indicated clinically noticeable (over the limit of 3.7) color change in all groups except for the EP-BN and T-BN groups.
Conclusion:
Although BN–Cr coating in this thickness had a negative effect on color, it increased the hardness of the ceramics and could be useful in the palatal region.
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Original Article:
Effect of Er:YAG laser radiation on pull-out fracture load of esthetic posts luted to root canal dentin with various resin cements
Horieh Moosavi, Farzaneh Ahrari, Maryam Zanjani
Dent Res J
2021, 18:17 (17 March 2021)
DOI
:10.4103/1735-3327.311424
Background:
This study investigated the influence of erbium-doped: yttrium aluminum garnet (Er:YAG) laser on the pull-out fracture load of fiber-reinforced composite (FRC) posts luted to dentin with different resin cements.
Materials and Methods:
In this
in vitro
experimental study, 90 premolars were endodontically treated. The post spaces were prepared, and the teeth were divided into three groups dependent on the cement applied for luting FRC posts: Group 1: An etch-and-rinse system, Group 2: A self-etch cement, and Group 3: A self-adhesive cement. After 6 months' storage and thermocycling, each group was divided into three subgroups (
n
= 10) according to the treatment applied for removing the posts; subgroup 1: Control, subgroup 2: Treatment with Er:YAG laser at 250 mJ, 20 Hz, and subgroup 3: Treatment by Er:YAG laser at 300 mJ, 10 Hz. The pull-out load was recorded in Newton. The data were analyzed by two-way ANOVA at
P
< 0.05.
Results:
The fracture load was significantly affected by the cementation group (
P
= 0.005) and treatment subgroup (
P
= 0.008). The pull-out load of self-etch cement was significantly greater than that of the self-adhesive and etch-and-rinse systems (
P
< 0.05). Treatment with Er:YAG laser caused a significant reduction in pull-out load of FRC posts (
P
< 0.05).
Conclusion:
The fracture load of fiber posts is influenced by the type of cement and treatment applied. Post removal would be less challenging when using a self-adhesive or conventional etch-and-rinse cement or using Er:YAG laser at the FRC-resin interface.
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Original Article:
The effect of Remin Pro on the microhardness of initial enamel lesions in primary teeth: An
in vitro
study
Homa Nourolahian, Iman Parisay, Fatemeh Mir
Dent Res J
2021, 18:16 (17 March 2021)
DOI
:10.4103/1735-3327.311423
Background:
Remineralization is currently considered a treatment option for many clinicians. The present study was conducted to compare the effect of three remineralizing agents on microhardness of initial caries lesions in primary teeth.
Materials and Methods:
In this
in vitro
study, 96 enamel samples were prepared. Microhardness was first measured for all the samples using the Vickers microhardness test. After developing the initial caries lesions, the microhardness of all the demineralized samples was measured, and the samples were then divided into four groups (
n
= 24). Casein phosphopeptide–amorphous calcium phosphate in Group 1, Remin Pro in Group 2, and acidulated phosphate fluoride gel in Group 3 were placed on the samples for 4 min. The control group received no treatments. The microhardness of the samples was measured again following a pH cycle of 5 days. The data were analyzed by ANOVA and the
post hoc
test at the significance level of
P
< 0.05.
Results:
The mean microhardness reduced significantly in all the groups following the development of initial caries lesions and after the pH cycle of 3 days (
P
< 0.001). After the remineralization and pH cycle of 5 days, the mean microhardness was significantly lower in the control group compared to the other three groups (
P
< 0.001) and had increased in the three treated groups. The microhardness recovery rate also increased in the treated groups compared to the control group, but no significant differences were observed between the three groups themselves (
P
> 0.05).
Conclusion:
Remin Pro can be used as an effective substance for preventing tooth caries in children.
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Case Report:
Canalicular adenoma of the hard palate: A rare case report
Milad Khodaei, Masoomeh Amani, Sina Mirinezhad, Sona Rafieyan
Dent Res J
2021, 18:15 (17 March 2021)
DOI
:10.4103/1735-3327.311422
Canalicular adenoma (CA) is a rare, benign salivary gland tumor that has special tendency to occur in the upper lip. Buccal mucosa is the second most common site. It occurs more often in older patients with peak prevalence in the seventh decade of life. A definitive female predominance has been reported. According to the latest English published literature, 531 cases of CA have been reported. We present a case of CA of the hard palate that presented in a 29-year-old female patient. The histopathological and immunohistochemical findings showed typical features of CA and intense expression of pan-cytokeratin and S-100 protein. We also review its differential diagnosis from other salivary gland tumors.
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Original Article:
Effect of cavity design and material type on fracture resistance and failure pattern of molars restored by computer-aided design/computer-aided manufacturing inlays/onlays
Roqaia Mohammad Alassar, Amira Mohammad Samy, Fatma Mahmoud Abdel-Rahman
Dent Res J
2021, 18:14 (17 March 2021)
DOI
:10.4103/1735-3327.311421
Background:
The maximum conservation of tooth structure and the use of restorative materials with elastic modulus close to the dental structure may promote greater longevity of the tooth/restoration complex. This study was conducted to evaluate the effect of cavity design and material type on fracture resistance and failure pattern of molars restored by computer-aided design/computer-aided manufacturing (CAD/CAM) inlays/onlays.
Materials and Methods:
In this
in vitro
study, 55 human maxillary molars were embedded in resin blocks and divided into control group (CG) and five main groups: Group 1: Inlay, Group 2: Conventional onlay/mesiobuccal (MB), Group 3: Conservative onlay/MB, Group 4: Conventional onlay/MB and distobuccal (DB), and Group 5: Conservative onlay/MB and DB. Then, each group was divided into two subgroups: (A) CeraSmart (CS) and (B) Katana Zirconia (KZ). Restorations were cemented by RelyX Ultimate and then thermocycled. The universal testing machine was used to measure fracture loads. Failure was determined using a magnifying lens. Data were statistically analyzed using ANOVA followed by Tukey's
post hoc
test (
P
< 0.05).
Results:
Group 5 showed the highest significant fracture load, whereas the least significant value was recorded in Group 2. KZ recorded higher significant fracture loads than CS in all tested groups. Groups 1, 2, and 3 restored by CS showed lower fracture load than CG, but the difference was insignificant with Group 1. CS restorations showed restorable failure, while unrestorable pattern was predominant in KZ restorations (
P
< 0.05).
Conclusion:
KZ inlays and onlays can be used safely in terms of fracture resistance as both have values exceed the physiologic requirements. CS inlays and onlays/MB and DB are of fracture resistance comparable to intact teeth. The use of conservative onlay design with more cusp coverage guarantees better resistance of CS restorations. Being force absorbing material, the predominant failure of teeth restored by CS was restorable.
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Original Article:
The effect of sodium hypochlorite enamel pretreatment on the shear bond strength of fissure sealant using a resin-modified glass ionomer cement and a fluoride-releasing self-etch resin adhesive
Najmeh Mohammadi, Ali Karimkhani, Rafat Bagheri, David J Manton
Dent Res J
2021, 18:13 (17 March 2021)
DOI
:10.4103/1735-3327.311420
Background:
The relative high caries risk of pits and fissures highlights the importance of protecting these areas. The aim is to determine the effect of sodium hypochlorite (NaOCl) on the shear bond strength (SBS) of resin-based pit and fissure sealant (RBPFS) material to enamel using resin-modified glass ionomer cement (RMGIC) and fluoride-releasing self-etch resin (FRSE) adhesives.
Materials and Methods:
In this
in vitro
study extracted third molar teeth without carious lesions or defects were divided into five experimental groups (
n
= 20). Group A: (Control group) etch (35% phosphoric acid) for 15 s and RBPFS applied. Group B: Etch for 15 s, FRSE and RBPFS applied. Group C: Pretreated with 5% NaOCl and similar steps to Group B. Group D: Etch for 15 s then RMGIC bonding agent and RBPFS applied. Group E: Pretreated with 5% NaOCl and then similar steps to Group D. SBS was determined using a universal testing machine. The tested specimens were examined under a field-emission scanning electron microscope. Data were analyzed using one-way ANOVA and post hoc Tukey's tests (
P
=0.05).
Results:
A statistically significant difference between the test groups was observed; Group C showed the highest SBS mean value (7.52 ± 2.74 MPa) and Group D showed the lowest (4.48 ± 1.81 MPa) (
P
< 0.001). Pretreatment with NaOCl increased the SBS of fissure sealant when Riva bond LC was used (
P
= 0.049).
Conclusion:
The use of NaOCl as pretreatment can increase the SBS of RBPFS to enamel using RMGIC adhesive. FRSE adhesive did not show improvement in SBS values using pretreatment.
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Original Article:
The relationship between temperament and behavior in 3–7-year-old children during dental treatment
Atousa Janeshin, Mohammmad Habibi
Dent Res J
2021, 18:12 (17 March 2021)
DOI
:10.4103/1735-3327.311419
Background:
Various factors affect child behavior in different situations such as in a dental clinic. The purpose of this study was to investigate the relationship between temperament and child behavior during routine dental treatment.
Materials and Methods:
The present study is a descriptive-analytic study that included 199 children aged 3–7-years from patients referred to a dental clinic of Guilan University of Medical Sciences in 2017–2018. The Children's Behavior Questionnaire, which is specific to children aged 3–7 years, was used to assess children's dental temperament. Children's behaviors during dental treatment were recorded using Frankl scale. Finally, the data were analyzed by the SPSS-16 statistical software.
Results:
About 47.9% of the samples were female, with a mean temperament score of 4.79 which was significantly higher than males (
P
< 0.05). Almost 2.4% of children had completely negative behaviors and 2.8% had a completely positive behavior. The mean scores of fear (
P
= 0.008) and anger (
P
= 0.004) in children with completely negative behavior were higher than those with completely positive behavior. Moreover, the average scores of inhibitory control (
P
= 0.003) and perceptual sensitivity (
P
= 0.001) in completely positive children were higher than completely negative children.
Conclusion:
The results of the present study showed that girls had a better mean score of temperament than boys. The inhibitory control and perceptual sensitivity in completely positive children were significantly higher than negative children. In addition, a higher mean score of fear and anger was observed in completely negative children.
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Review Article:
COVID-19: Implications on dental profession and precautionary guidelines
Rajashree Dhananjay Jadhav, Arti Rajaram Gachake, Vasanthi V Swami, Abhishek Dhananjay Jadhav
Dent Res J
2021, 18:11 (17 March 2021)
DOI
:10.4103/1735-3327.311418
The current global spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its associated coronavirus disease (COVID-19), has grasped the attention of entire international community and has caused widespread public health concerns. All the efforts taken globally to curtail the novel COVID-19; the outbreak is still on a rise owing to the community spread pattern of this infection. COVID-19 is a zoonotic infection that, like other coronavirus infections, is believed to have been originated in bats and pangolins, and later transmitted to humans. Once in the human body, this coronavirus (SARS-CoV-2) is abundantly present in nasopharyngeal and salivary secretions of affected patients and its spread is predominantly thought to be respiratory droplet in nature. Dental professionals may soon encounter patients with suspected or confirmed SARS-CoV-2 infection and will have to act carefully not only to provide care but at the same time prevent spread of infection. Thus, the aim of this article is to provide a brief overview of the epidemiology, symptoms and routes of spread of the novel COVID-19 infection. In addition to this, specific recommendations for dental practice are prescribed for patient screening, infection control strategies, and patient management protocol.
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Original Article:
Stress distribution of esthetic posts in the restored maxillary central incisor: Three-dimensional finite-element analysis
Sina Jafari, Mohammad Alihemmati, Ali Jamali Ghomi, Sayed Shojaedin Shayegh, Kamran Kargar
Dent Res J
2021, 18:10 (23 February 2021)
DOI
:10.4103/1735-3327.310039
Background:
Glass fiber posts, along with their esthetic properties, may have a better stress distribution than cast posts, Therefore, the aim of the present study was to investigate the effect of glass fiber, casting, titanium, and zirconia posts on stress distribution in maxillary central tooth treated with different amounts of ferrule using finite-element analysis.
Materials and Methods:
In this experimental study, three-dimensional models of maxillary central incisors that have undergone root canal treatment were designed. Then, the models were divided into four groups according to the type of post (Ni-Cr casting, glass fiber, titanium, and zirconia) used. Zirconia monolithic crowns were used in all the four groups. Ferrule heights were repeated at 0 and 2 mm in all models. Models were entered into COMSOL Metaphysics software. Then, the force of 100 N with the angle of 135
°
on the palatal surface was applied to the longitudinal axis of the tooth, and the stress distribution in the models was investigated.
Results:
Maximum stress was observed in the middle third of posts. Stress distribution in glass fiber post was better than zirconia and casting posts. Stress accumulation in models with zirconia, titanium, and casting posts was also found in the site between the middle third and coronal third, whereas in models with glass fiber post, stress accumulation was found between the crown and the cemento-enamel junction. In models without ferrule, stress accumulation was observed in one-third of the coronal, especially in glass fiber posts.
Conclusion:
The post material and ferrule height affected stress concentration. The stress in the cervical area of the dentin was more for glass fiber post when compared to other posts. The use of glass fiber post in teeth with no ferrule results in lower stress along the post, but greater stress in the simulated tooth region.
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Original Article:
Effect of different surface treatment with panaviaV5 on shear bond strength of metal brackets to silver amalgam
Shiva Alavi, Farzaneh Shirani, Zahra Zarei, Seyed Amir Hossein Raji
Dent Res J
2021, 18:9 (23 February 2021)
DOI
:10.4103/1735-3327.310038
Background:
This study was conducted to compare the shear bond strength (SBS) of orthodontic brackets to amalgam surfaces by two surface treatment methods, two different adhesives, and one intermediate resin and also to evaluate surface roughness after two preparation methods as well as bond failure mode.
Materials and Methods:
In this
in-vitro
study forty-eight amalgam samples were randomly allocated to four groups. In Groups 1–3, specimens were sandblasted with 50 μm aluminum oxide, followed by application of Alloy primer in Groups 1 and 2. In Group 3 Alloy primer had not used. In Group 4, samples were prepared by silica coating using a silane coupling agent. Surface roughness analysis was performed in 10 additional samples after two surface treatments. The brackets in Group 1 were bonded with Transbond XT and those in other groups were bonded with Panavia V5. All specimens were examined for SBS following 5000 times thermocycling at 5°C–50°C. Modified adhesive remnant index was utilized for the bond failure mode. Data analysis was done by one-way analysis of variance,
post hoc
Tukey, Kruskal–Walli and Mann–Whitney U tests. Statistical significance was set at
P
< 0.05.
Results:
The findings indicated the mean SBS were low (ranged from 0.19 to 4.66 MPa) and significantly lower in Group 3 than in Group 4 (
P
= 0.009). Bond failure occurred in adhesive/amalgam interface in nearly all samples. Silica coating produced significantly lower roughness than sandblast (
P
= 0.009).
Conclusion:
Silica coating had a significant higher bond strength than sandblast without application of Alloy primer. However compared to sandblast with Alloy primer, silica coating did not significantly improve the bond strength. Chemical bond between PanaviaV5 and sandblasted amalgam was not considerable.
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Original Article:
Biocompatibility of several colloidal solutions containing nanoparticles on human gingival fibroblasts
Neda Eslami, Fatemeh Fasihi, Abdollah Jamalinasab, Farzaneh Ahrari
Dent Res J
2021, 18:8 (23 February 2021)
DOI
:10.4103/1735-3327.310037
Background:
There is little information concerning the biocompatibility of mouthwashes containing metal nanoparticles. This study was conducted to assess the biocompatibility of colloidal solutions containing zinc oxide (ZnO), copper oxide (CuO), titanium dioxide (TiO2), and silver (Ag) nanoparticles compared with chlorhexidine (CHX) in a culture of human gingival fibroblasts (HGFs).
Materials and Methods:
This was an
in vitro
, experimental study. Nanoparticles, including ZnO, CuO, TiO2, and Ag, were purchased and added to a water-based solution to produce mouthwashes. The colloidal solutions and CHX were prepared at the minimum inhibitory concentration (MIC) against
Streptococcus mutans
and
Streptococcus sanguis
. Cytotoxicity was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay on HGFs at the concentrations of MIC, 0.1 MIC, and 0.01 MIC. To determine apoptosis, DNA fragmentation was assessed as “sub-G1” peak on DNA content histogram. The data were analyzed using repeated measures analysis at
P
< 0.05.
Results:
At all concentrations, the highest and lowest mean of cell viability was related to TiO2 and ZnO groups, respectively. At MIC, the mean cell viability was significantly greater in the TiO2 group than the other groups (except the Ag group) (
P
< 0.05). At the concentration of 0.01 MIC, the mean cell viability in the colloidal solution containing ZnO nanoparticles was significantly lower than the other solutions (
P
< 0.05). The CHX and CuO-containing solution displayed the highest rate of apoptosis among the groups.
Conclusion:
The TiO2-containing solution can be suggested as a suitable alternative to CHX to provide antiseptic effects with minimal toxicity.
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Original Article:
Relationship of pH and the viscosity of five different iron supplements with the absorption of iron ions and enamel discoloration in the anterior primary teeth (an
in vitro
study)
Navid Babaei, Tina Molaei, Saeid Belyad, Somayeh Hekmatfar
Dent Res J
2021, 18:7 (23 February 2021)
DOI
:10.4103/1735-3327.310036
Background:
Various iron drops are prescribed to children to prevent iron-deficiency anemia. The current study aimed to evaluate and compare the physicochemical profile of iron drops and the effect of these medicines on the color of primary teeth.
Materials and Methods:
On the commencement of this experimental study, the pH and viscosity of five types of iron drops were measured. For the purpose of the current study, sixty healthy anterior deciduous teeth were provided; thereafter, they were assigned to five groups and then exposed to iron drops. The color and color difference of each specimen were measured by VİTA Easyshade Compact after 2 weeks. The amount of absorbed iron was determined by atomic absorption. The data were analyzed using two-way ANOVA and Tukey's test (
P
< 0.05).
Results:
As evidenced by the obtained results, all medications demonstrated acidic pH and discoloration. The viscosity values of iron drops were reported to be within the range of 2.07–33.58 cP. Based on the results of Pearson's correlation coefficient test, discoloration showed a correlation with pH and atomic absorption (
P
< 0.05).
Conclusion:
Analyzed iron drops displayed low pH and discoloration higher than 3.3 which can be easily distinguished with naked eyes. Medicine labels should include warning statements on the feasibility of dental discoloration and erosion.
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Original Article:
Comparison of information provided by pediatricians regarding tooth eruption and the information available on the internet
Maryam HajiAhmadi, Najmeh Akhlaghi, Faezeh Aghajani, Hadi Moshgelgosha, Mahshad Soltanian
Dent Res J
2021, 18:6 (23 February 2021)
DOI
:10.4103/1735-3327.310035
Background:
Most parents believe that teething is associated with some symptoms and commonly use Internet to gain more information about the health of their children. Pediatricians can also serve as a source of information for health-related requirements of parents. This study aimed to compare the information provided by pediatricians regarding tooth eruption and the information available in Persian-language websites.
Materials and Methods:
This descriptive, analytical, cross-sectional study was performed in two phases. In the first phase, the questionnaire was designed by 85 pediatric pediatricians in Isfahan about children's dental eruption and internet resources. The second phase was the study of 22 Persian websites about children's tooth eruption using Google search engine and the keywords “tooth eruption,” “tooth eruption symptoms.” Then a checklist was prepared to evaluate the quality and completeness of the content. Data were analyzed by the Chi-square test and Fisher's exact test using SPSS (
P
< 0.05).
Results:
None of the retrieved websites had the health on the net code of conduct. The most common symptoms of teething reported by both pediatricians and websites included irritability, drooling, gingival tenderness, chewing tendency, decreased appetite and sleep disturbances. In non-pharmacological methods to control the symptoms related to tooth eruption in both groups, embracing the baby (
P
< 0.001), using soft tooth ring (
P
= 0.049) and chewing hard foods (0.016
P
= significant. Use of oral pain medication (
P
= 0.01) and sedatives and medications (
P
= 0.04) were significant in the medication method.
Conclusion:
None of the websites reviewed had a valid source code and author. Although websites cannot replace the instructions of pediatricians regarding growth and development of children and their teething, supplementary information can be acquired from high-quality websites because there seems to be no significant difference between these two information sources regarding tooth eruption symptoms and their management.
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Original Article:
Effect of immersion in hydrochloric acid and sodium hypochlorite and autoclave sterilization on the force characteristics of orthodontic nickel-titanium open coils
Saeed Noorollahian, Mehrnaz Zakizade
Dent Res J
2021, 18:5 (23 February 2021)
DOI
:10.4103/1735-3327.310034
Background:
Nickel-titanium (NiTi) open coils are common tools in fixed orthodontic treatments to apply light and continuous forces. Their favorable mechanical properties and their relatively high cost have prompted many clinicians to decide to reuse them.
Aim:
The present study aimed to evaluate the effect of 10% hydrochloric acid (HCl) and 5.25% sodium hypochlorite (NaClO) solution and sterilization, on the unloading force of NiTi open-coils.
Materials and Methods:
This experimental
in vitro
study consisted of 160 NiTi open coils from two brands of Highland Metals and Masel were provided. Each group was randomly divided into four subgroups: control, three rounds of immersion, three rounds of autoclave sterilization, and three rounds of immersion and autoclaving. Each round of immersion process included 1 min in 10% HCl, followed by 5 min in 5.25% NaClO. Spring was compressed for 4 mm. Then, the unloading forces of each spring were recorded at 4, 3, 2, and 1 mm of compression, respectively. The data were analyzed with SPSS (
P
< 0.05), using one-way ANOVA and independent
t
-test.
Results:
One-way ANOVA showed a significant difference in the 2-mm compression of Group 1 and 3-mm compressions of Groups 1 and 2 compared to the control group in the Highland metals brand (
P
< 0.04,
P
< 0.014, and
P
< 0.007, respectively). There was no significant difference between the different compressed lengths and groups in the Masel brand.
Conclusion:
One-minute immersion in 10% HCL and 5-min immersion in 5.25% NaOCl, followed by autoclave sterilization, even after repeating the process three times, did not significantly change the force properties of NiTi open springs. Therefore, the re-use of these springs with the above-mentioned method as preparation can be recommended.
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Original Article:
Oral manifestations in inflammatory bowel disease: A cross-sectional study in Isfahan
Faezeh Khozeimeh, Hadi Shakerin, Hamed Daghaghzadeh, Fereshteh Najarzadegan, Zahra Golestannejad, Peyman Adibi
Dent Res J
2021, 18:4 (23 February 2021)
DOI
:10.4103/1735-3327.310033
Background:
Various systemic diseases can cause oral manifestations, such as inflammatory bowel disease (IBD). This study is designed to investigate the frequency of oral manifestations in patients with IBD referred to health centers and offices in Isfahan in 2018.
Materials and Methods:
This cross-sectional study was done among 161 patients suffering from IBD in Isfahan, Iran. They were chosen by the systematic randomized sampling. Finally, the data were analyzed using the logistic regression test in the SPSS software. Significance was assigned at
P
< 0.05.
Results:
The number of patients with ulcerative colitis and Crohn's syndrome was, respectively, 119 persons (73.9%) and 42 persons (26.1%). Oral manifestations were seen among 52 (32.3%) of the samples. The frequency of oral manifestations was 35.6% (31 cases) in males and 28.4% (21 cases) in females. Oral manifestations were seen in 29.4% of patients with ulcerative colitis and 40.5% of patients with Crohn's disease. Based on the logistic regression, there was a statistically significant relationship between the use of azathioprine and mesalazine with oral manifestations (
P
< 0.05), whereas the severity of disease and smoking were not statistically significantly related to oral manifestations (
P
> 0.05).
Conclusion:
The severity of the disease was not significantly correlated with oral manifestations, which are in agreement with the results of previous studies. It was also found that among patients with IBD, the oral aphthous ulcers can appear 1–3 years before the diagnosis of the disease. Besides that, some of the drugs used to treat the disease have a significant relationship with oral manifestations.
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Original Article:
In vitro
antimicrobial activity of mineral trioxide aggregate, Biodentine, and calcium-enriched mixture cement against
Enterococcus faecalis, Streptococcus mutans
, and
Candida albicans
using the agar diffusion technique
Parinaz Esteki, Maryam Zare Jahromi, Arezoo Tahmourespour
Dent Res J
2021, 18:3 (23 February 2021)
DOI
:10.4103/1735-3327.310032
Background:
This study assessed the antimicrobial activity of Biodentine, mineral trioxide aggregate (MTA), and calcium-enriched mixture (CEM) cement against
Enterococcus faecalis, Streptococcus mutans
, and
Candida albicans
.
Materials and Methods:
In this
in vitro
study, microbial suspensions were inoculated onto agar plates. The antimicrobial effects of MTA, Biodentine and CEM cement were assessed against
E. faecalis, S. mutans
, and
C. albicans
by the agar diffusion test. In each experimental group, 7 plates containing 3 wells were prepared and immediately filled with freshly mixed cements. Positive and negative control plates were prepared with/without the bacterial suspension, respectively. After 2 h of preincubation at room temperature, the plates were incubated at 37°C for 24 h. The diameter of growth inhibition zones was measured after 24 h. Data were analyzed using ANOVA and Tukey's test (α = 0.05).
Results:
Biodentine showed strong antimicrobial activity against all three microorganisms with an average inhibition zone of 9.10 mm. The inhibitory effect of Biodentine on
E. faecalis
and
C. albicans
was significantly superior to that of the other two cements (
P
< 0.05). MTA and CEM cement showed significantly higher antimicrobial activity against
S. mutans
(
P
< 0.05). The antimicrobial effects of Biodentine on
S. mutans
and
E. faecalis
were significantly greater than on
C. albicans
(
P
< 0.05).
Conclusion:
All cements revealed antimicrobial properties against the tested microbial strains. Biodentine had stronger antimicrobial effects against
E. faecalis
and
C. albicans
compared with MTA and CEM cement. Furthermore, the largest inhibition zones around all three cements belonged to
S. mutans
.
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Original Article:
Long-term effect of acidic pH on the surface microhardness of ProRoot mineral trioxide aggregate, Biodentine, and total fill root repair material putty
Koral Bayraktar, Fatima Betul Basturk, Dilek Turkaydin, Mahir Gunday
Dent Res J
2021, 18:2 (23 February 2021)
DOI
:10.4103/1735-3327.310030
Background:
The purpose of this study was to compare the microhardness values of ProRoot mineral trioxide aggregate (MTA), Biodentine, and total fill root repair material (TF-RRM) Putty at varying pH and times.
Materials and Methods:
In this laboratory experiment, materials were mixed and placed in cylinder blocks with internal dimensions of 6 mm × 4 mm. Ten samples of each material were soaked in buffered solutions of butyric acid with 4.4, 5.4, 6.4, and 7.4 pH values and stored at 37°C in 100% humidity. The samples were submitted to the microhardness test at the end of 1 week and then 1 month. Multivariate analysis of variance and Tukey honestly significant difference tests were carried out to compare the mean values at a significance level of
P
< 0.05.
Results:
Low pH caused a significant decrease in the microhardness values of all samples. Surface microhardness increased with time (
P
< 0.0001). The microhardness values of Biodentine were significantly greater than those of ProRoot MTA and TF-RRM putty (
P
< 0.0001). The lowest microhardness values were recorded for TF-RRM putty groups regardless of the pH of the environment and the evaluation time.
Conclusion:
An acidic environment impaired the surface microhardness of all root repair materials tested. Overall, the mean surface microhardness of TF-RRM Putty was lower than those of ProRoot MTA and Biodentine. Biodentine showed the greatest microhardness values at all pH values, regardless of the evaluation time.
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Original Article:
Finite element analysis of the effect of framework materials at the bone–implant interface in the all-on-four implant system
Kasturi Chandrashekhar Kelkar, Vinaya Bhat, Chethan Hegde
Dent Res J
2021, 18:1 (23 February 2021)
DOI
:10.4103/1735-3327.310031
Background:
The “All-on-four” concept for treatment of edentulous arches incorporates four implants that are placed in between mental foramina in the mandible. The prosthetic framework is an important parameter in stress/strain concentration at the implants, prosthesis, and the underlying bone. Materials such as titanium, zirconia, and carbon fibers have been used for fabrication of framework in the past. The aim of this study was to analyze the effect of framework materials in the “All-on-four” implant system.
Materials and Methods:
Finite element three-dimensional (3D) model of edentulous mandible was simulated using a computerized tomographic scan data of an edentulous patient. Threaded implants were replicated along with the abutments using 3D modeling software and the framework was designed and simulated using material properties of titanium, zirconia, and polyetheretherketone (PEEK). Axial and nonaxial load of 200 N was applied at the abutment region of right distal implants. The computer-generated numerical values were tabulated and analysed by ANSYS software.
Results:
Principal strain, von Mises stress and micromotion were assessed in the peri-implant bone region to evaluate its stress condition. Zirconia framework showed the least stress/strain values at axial and oblique loading. Maximum strain values were seen at the PEEK framework material. Zirconia framework in all models showed the least micromotion/displacement.
Conclusion:
The stress distribution pattern at implant–bone interface was influenced by the framework material used. The framework material, loading site, and direction of forces influenced the stresses and displacement at the bone–implant interface.
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